实验

This equation was used to predict DNA 〓 under other electrophoretic conditions, the relative error is less than +5%.

利用少量的实验数据通过该公式所确定的有关参数进行实验预测，预测值与实验值的相对误差均在±5％以内。

Inflammatory cells in the hematoma expressed bFGF and proliferating periosteal cells, endosteal cells, medullary mesenchymal cells expressed TGF-β at 3 days postoperatively.

3种生长因子在实验侧和对照侧的含量有明显差别。实验侧总体含量明显高于对照侧(P.01)，各个时间组实验侧含量也高于对照侧。

Experiment Road Choose the heavy highland road of the mountain range as experiment, the alignment flectional degree varies from simple (includes the expressway not run) to complicated, sums to 14 zones which the distance is 800ms or so.

实验道路以山岭重丘区的道路为实验道路。线形弯曲程度由简单(包括一段未开通的高速道路)到复杂，区间长分别为800m左右的14个区间为实验道路。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Degradation test in vitro was carried out in phosphate buffer solution (0.1M, pH7. 4) at 37 ℃. The buffer solution was changed daily. Degradation test in vivo was implanted the sample to subdermal in adult ICH rat in the scapular area lateral to the dorsal midline. At suitable time the samples were recovered. Molecular weight changes in surface layer and bulk of polymer sample were measured by GPC and weight loss was determined gravimetrically. It was found that the degradation behavior can be regulated by changing the composition of copolymers. The critical compositions from surface to bulk degradation behavior for PGCA, PLCA, PLMCA, PLDCA systems were 15-20, 20-30, 30- 40, 40 of mol percent GA or LA unit in copolymers, respectively. The degradation behavior of PGCA, PLCA, PLMCA, PLDCA systems were compared and analysed. Some factors influencing the degradation character, such as copolymer composition, hydrophobicity, crystallinity and enzyme affect etc. played important role.

体外实验中材料降解环境为37℃，0.1M，pH7.4磷酸缓冲溶液，每天换液，定期取样；体内实验中将聚合物试样埋置于ICH小白鼠背部肩胛骨皮下部位，定期处死小鼠，取样，将体内体外样品进行重量损失及试样内外层分子量变化测定，分析各聚合物试样降解行为特性，实验结果证明，改变共聚物组成，可以调节各聚合体系降解行为特性，对PGCA，PLCA，PLMCA，PLDCA共聚体系，交酯摩尔百分含量15-20％，20-30％，30-40％，40％分别为各体系内降解行为特性由表面降解型向本体降解型过渡的临界转折点，交酯含量较低的聚合物不同程度地表现表面降解行为特性，论文对各共聚体系体内外降解行为作了分析对照，例如共聚物组成对材料降解速度与降解行为的影响；生物体内酶对降解行为的影响；材料亲疏水性，聚合物链段结晶行为及碳酸酯结构对材料降解行为的影响等，得出交酯/环碳酸酯共聚体系降解行为一些共性和规律。

By analyzing these data,it was concluded that:1The combustion of wood,including rate of heat release,total heat release,effective heat of combustion,mass loss rate,smoke ratio,specific extinction area,concentration of CO_2 and the yield of CO_2Y_(CO2,were decreased considerably by the treatment of FRW;2There is a strong synergistic effect of fire retardance between GUP and boric acid;3The trend of MLR curve of FZ was similar to its RHR curve,weight loss and heat release were happened mainly in flaming phase;4Char formation of wood was markedly accelerated by FRW,which indicated that catalytic charring effect of FRW on wood is the primary side in its fire-retardation mechanism.

对于组成和结构变异性很大的木材而言，由于CONE实验所使用的样品量相对于其他分析方法要大得多，因而实验结果比较有代表性。此外，CONE实验可在模拟火灾条件下进行，这是该方法的又一突出的优点[5～6]。木材阻燃剂FRW主要由磷酸脒基脲和硼酸组成，此外还含有

First and final drafts of the 30 students were scored holistically, and the number of moves of job application in the two drafts was counted and compared.

参加实验的30名学生每人在实验前和实验后根据所给的不同情景分别写了一篇求职信。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

On the base of the brief description of the drive and control system of micro-fluid and the experimental setting of the technology of micro-injection, a novel process of the hydrophobe treating used to mend the characteristic of the inner and outer surface on micro-pipette is designed, which can improve greatly the injected effect and eliminate the disadvantage such as seepage or regurgitation during injection. The author puts forward the way of measuring the inside diameter of the micropipette. The program of measuring micro injection amount is designed. The original data of the micro injection experiment are explained and interpreted at last in the thesis.

在简要描述微流体驱动控制系统和微注射技术实验条件的基础上，设计了改善微针内外表面特性的疏水化处理工艺，消除了微喷射过程中的渗流、回流现象，改善了微注射过程中的流动特性，提高了微注射效果，得到了数字化微喷射的实验结果；提出了精确测量微针内径的折射率法；设计了纳升级以下的微液滴量的检测方案；最后，论文对微注射技术实验中的原始数据进行了分析。

This research mainly adopts experiment, survey questionnaire, interview method, the experimental chemistry of several typical topic such as "separation of iodic element of kelp and inspection" and the properties of aluminum compounds "toothpaste and match in some ingredients" sodium nitrite and identification of salt experimental subjects were studied.

本研究主要采用实验法、调查问卷法、访谈法，对《实验化学》中的几个典型课题如&海带中碘元素的分离及检验&&铝及其化合物的性质&&牙膏和火柴头中某些成分的检验&&亚硝酸钠和食盐的鉴别&等实验课题进行研究。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。