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Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer, In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed.

加入诱导剂RU486后,可以诱导表达荧光素酶,并在一定范围内两者呈正比,最高可以实现荧光素酶的40余倍的表达,而没有RU486时,几乎没有报告基因的表达,表明RU486诱导调控载体构建成功,可实现对目的基因的表达时间和表达水平的精确调控,为进一步的基因调控研究和基因治疗提供了良好的工具。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后,可以诱导表达荧光素酶,并在一定范围内两者呈正比,最高可以实现荧光素酶的40余倍的表达,而没有RU486时,几乎没有报告基因的表达,表明RU486诱导调控载体构建成功,可实现对目的基因的表达时间和表达水平的精确调控,为进一步的基因调控研究和和基因治疗提供了良好的工具。

SDRAM is used as image storage, which can store many frames image data to provide the foundation for the correlation operation between frames. FIFO is a data buffer for real-time image. All of these make the image processing system simple design, decreasing the write-read time and solving the question of transmission and delay, and allowing the data processing and the data grabbing at the same time. The processed results can be output in 20ms, so the bandwidth of the C6000-based image processing system is improved.

系统采用SDRAM作为图像存储器,可以存储多帧图像数据,为实现帧相关运算奠定了基础,实时图像数据采用FIFO作为缓存,使系统设计简单,读取速度快,解决了数据传输的瓶径问题,同时也减少了传输延时,实现了对图像数据边采集边处理,减少了系统的滞后时间,当场给出电视脱靶量,提高了图像处理系统的带宽。

The results showed that the algorithm realizes the watermark's imbedding and extracting; it also is robust. The digital watermarking technology can be used to protect the security of digital images and the copyright.

结果证明该算法能够实现数字水印的提取和检测,并具有较强抵抗常见攻击的能力,鲁棒性较好,能够实现初步的数字图像的防伪和版权保护。

The results showed that the algorithm realizes the wa'termark's imbedding and ex-tracting; it also is robust. The digital watermarking technology can be used to protect the security of digital im-ages and the copyright.

结果证明该算法能够实现数字水印的提取和检测,并具有较强抵抗常见攻击的能力,鲁棒性较好,能够实现初步的数字图像的防伪和版权保护。

The Guidant-independent Teaching Model of High School Biology sticks to the principle of student-oriented so as to build the classroom atmosphere of democratic, fair, free and open, active the effective teaching interaction and enable students to participate actively in classroom teaching and develop the activeness and independence of students. The Guidant-independent Teaching Model adheres to the rule of students-centered. It proposes teaching-after-self-learning, teaching-follow-self-learning, teaching-promote-self-learning and enables students to learn by themselves at first through reading, self-thinking, questioning, group-discussion and forum.Thus, the independence and imitativeness of students will be achieved.We also lay emphasis on the development of the abilities of self-study and co-operative study so as to get the teaching goal of "learn how to learn" and promote the development of the independence of students.

引导自主式高中生物课堂教学模式坚持以学生为主体,通过营造民主平等、自由开放的课堂教学氛围,实现有效的教学交往和互动,让学生主动参与到课堂教学中来,以发挥学生主体的积极性、主动性;引导自主式高中生物课堂教学模式坚持以学生为中心,主张先学后教、以学定教、以教促学,通过阅读、独立思考、质疑、小组讨论和集中交流等环节让学生自主先学,以充分发挥学生主体的能动性、创造性,培养学生自主学习和合作学习等能力,从而实现学会学习的教学目标和促进学生主体性的发展。

Factors that could affect results include the ability to market and sell products; changes in relationships with strategic partners and reliance on strategic partners for the performance of critical activities under collaborative arrangements; failure of distributors or other customers to meet purchase forecasts or minimum purchase requirements for our products; impact of competitors, competing products and technology changes; ability to develop, commercialize and market new products; market acceptance of oral fluid testing or other new products or technology; changes in market acceptance based on product performance; continued bulk purchases by customers, including governmental agencies, and the ability to fully deploy those purchases in a timely manner; ability to fund research and development and other products and operations; ability to obtain and maintain new or existing product distribution channels; reliance on sole supply sources for critical product components; availability of related products produced by third parties; ability to obtain, and timing and cost of obtaining, necessary regulatory approval for new products or new indications or applications for existing products; ability to comply with applicable regulatory requirements; history of losses and ability to achieve sustained profitability; volatility of our stock price; uncertainty relating to patent protection and potential patent infringement claims; uncertainty and costs of litigation relating to patents and other intellectual property; availability of licenses to patents or other technology; ability to enter into international manufacturing agreements; obstacles to international marketing and manufacturing of products; ability to sell products internationally; loss or impairment of sources of capital; ability to meet financial covenants in agreements with financial institutions; ability to retain qualified personnel; exposure to product liability, patent infringement, and other types of litigation; changes in international, federal or state laws and regulations; customer consolidations and inventory practices; equipment failures and ability to obtain needed raw materials and components; the impact of terrorist attacks and civil unrest; ability to complete consolidation or restructuring activities; ability to identify, complete and realize the full benefits of potential acquisitions; and general political, business and economic conditions.

可能影响结果的因素包括营销以及出售产品的能力;与战略合作伙伴关系的变化以及根据合作协议对战略合作伙伴执行关键活动的依赖;经销商或者其他顾客未达到我方产品的采购预测或者最低采购要求;竞争对手、竞争产品以及技术变化的影响;新产品开发、商业化以及营销的能力;市场对唾液检测、其他新产品或者技术的接收程度、基于产品效果所造成的市场接收的变化;顾客、包括政府机构的持续大宗采购以及按时完成采购的能力;资助研究、开发以及其他产品和活动的能力;获得并维持新的或者现有的产品经销渠道的能力;关键产品部件对单一供应源的依赖;第三方生产的相关产品的供应情况;新产品或者现有产品的新疗效获得必要的管制批准的能力、时间以及费用;遵守适用的管制要求的能力;亏损的历史以及实现可持续盈利的能力;股票价格的波动;与专利保护和潜在的专利侵权诉讼有关的不确定性;与专利以及其他知识产权有关的不确定性以及诉讼费用;专利以及其他技术授权的提供情况;签订国际性制造协议的能力;产品进行国际营销以及制造的障碍;国际性销售产品的能力;资本来源的损失或者减少;履行与财务机构之间协议的能力;获得合格人员的能力;产品责任、专利侵权以及其他诉讼的风险;国际、联邦或者州的法律、法规的变化;顾客合并以及存货实践;设备故障以及获得所需原材料和部件的能力;恐怖主义袭击以及内乱的影响;完成合并或者重组活动的能力;识别、完成以及实现潜在收购的全部利益的能力;一般性政治、商业、经济形势等。

One is the extent permitted by the imperialist breakthrough to achieve national independence, in order to achieve modernization.

一种是突破帝国主义许可的范围,争取实现民族独立自主,从而实现现代化。

And, the issues involving the implemental principle of SDAI in the STEP standard are resolved.

论文描述了SDAI的原理及其实现方式,并阐述了本论文采用的SDAI实现方式。

Consequently a hardware implementation scheme of the whole system and all modules are proposed. Furthermore, the key implemental methods are presented in detail.

在此基础上,进而提出整个系统和各模块的硬件实现方案,给出关键问题的具体实现过程。

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It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为!

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