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Objective To establish a method for quantitative detection of cytotoxin associated gene A positive Helicobacter pylori by competitive polymerase chain reaction.

目的 建立竞争性聚合酶链反应定量检测幽门螺杆菌细胞毒素相关蛋白基因A的方法。

HP were determined by rapid urease test,Warthin-Starry silver stain and polyme rase chain reaction,and expression of P21,P53,C-myc and C-erbB-2 were detecte d by immunohistochemicology (Streptaovidin/Peroxidase,SP) method,and were evalua ted with positive expressive integrality,the mean optical density an d the integral optical density in computer assisted image analysis system.

方法经内镜和病理诊断的胃癌91例,用快速尿素酶试验,改良W-S银染色和PCR方法检测上述标本中Hp;以S-P免疫组织化学法检测P21、P5 3、C-mcy及C-erbB-2,并用阳性表达积分半定量和免疫组化图像分析进行比较。

The present study wastherefore designed to examine the molecular toxic effect of MC-LR on bighead carpAristichthys nobilis and zebrafish Danio rerio by using real-time polymerase chainreaction, molecular cloning and immunoblotting.

本研究以斑马鱼和鳙鱼为对象,采用实时荧光定量PCR、分子克隆和免疫印迹技术在分子水平上揭示了MC-LR对鱼类的毒理效应。

The quantitative and qualitative analyses of data point to three major findings.

研究采用定量和定性相结合的方法分析数据。

Decidua samples from the two groups were obtained at the time of abortion.

我们通过对tnfr1在蜕膜组织中表达的定位和半定量研究。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型,连续灌胃6周,处死动物取血,检测血清ALT、AST、ALB;ELISA法测定血清透明质酸;取肝、睥,并计算肝、脾指数,组织切片HE染色后进行组织病理学疗效评估;用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达;半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

One is that "avoided deforestation" is hard to define and quantify.

其中之一就是很难为"被避免的去森林化"定性和定量。

Handan City-kun jump processing machinery Technology Co., Ltd. is committed to the development of magnetic equipment, manufacturing, product high Qiangci stem the election machine, demagnetizer, dehydration magnetic separator, magnetic separation column, DGS-Screen-high-frequency electromagnetic, magnetic pulley Qiangci , Adjustable magnetic reunion gravity concentrator, permanent magnet filter vacuum, magnetic separator, automatic weighing quantitative feeding machines.

邯郸市坤跃选矿机械科技有限公司致力于磁力设备的研发、制造,产品有高强磁干选机、脱磁器、脱水磁选机、磁选柱、DGS型电磁高频振网筛、强磁磁滑轮、变径型磁团聚重力选矿机、真空永磁过滤机、磁选机、自动称重定量给料机等。

The application of QLF in clinic diagnosis and quantitative demineralization analysis[D].

尹伟。定量光导荧光技术在临床诊断和定量分析脱矿中的应用研究[D]。

6Exploration of the nature of denervation muscle atrophy and its counter measures.

揭示肌萎缩的本质,找到定量指标。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力