定量的

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛，包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等，已广泛应用于医学临床，它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

Based on the study of DNA isolation from different samples, this paper applied polymerase chain reaction and PCR-derived technology, and utilized many kinds of known samples and reference materials (such as soybean, maize, rice, potato, and pimiento, etc.) to establish the qualitative, half-quantitative, and quantitative GMO detection method: 1. We established a qualitative PCR screening method for CaMV 35S promoter and Tnos terminator. It must be taken into account that false positive results from screening method.

在DNA提取方法研究的基础上，本文应用聚合酶链式反应(polymerasechain reaction，PCR)及其衍生技术，利用大豆、玉米、水稻、马铃薯、甜椒等多种已知标准样品，建立了多种农作物和植物源性食品的GMO定性、半定量和定量检测方法： 1、在单基因定性检测技术方面，针对现有的商品化GMO中大多数含有花椰菜花叶病毒35S（CaMV 35S）启动子和胭脂碱合成酶基因终止子的特点，本文建立了CaMV 35S启动子和Tnos终止子的定性PCR筛选检测方法。

In hypergensis process uranium tend to be activate and transfer while thorium tend to enrich in the relict phase.

在我们的研究中，通过计算，得到了放射性核素浓度与核素的比放射性活度之间的定量关系，进一步推算出α、β比放射性活度与核素浓度之间的定量换算公式，这对放射性环境监测具有一定的实际意义。

The aim of this paper is to summarize the experiences of the construction of coal harbors, forecast the harbor throughout and shipping quantity based the main effect of the sea coal market by the investigation of the equipment of coal harbors, give the coal harbor evaluating index and methods, and analyze the foreground and countermeasure of the harbors based the system innovation by the developing forecast of coal market and the capacity analyze of coal harbors.The paper uses the methods of determining the nature and quantity.

本项研究采用定性与定量向结合的方法；煤炭海运量预测以未来煤炭市场需求预测为依据，按照主要煤炭调入地区运输方式的分配比例，并根据煤炭运输的经济性和合理性原则，采用指数平滑预测方法进行预测；对港口通过能力和设施、设备的利用情况进行定量评价，并建立相关的评价模型和评价指标。

For firms' adoption behavior, this thesis firstly explains why firms' adoption is in order from the static state by analyzing the regular pattern of firms' adoption behavior; builds control model of firms' adoption decision behavior, and describes the relationship between the stable values of response probability of firms' adoption decision; secondly raises the conception of firms' states from the dynamic state and defines two state variables to explain why and how a firm changes from nonadoption to adoption through the dynamic analysis of the factors influencing the two state variables. Thirdly, constructs the functions of adopting and choosing behavior with mathematical method, which is verified in following.

对于企业采用行为，首先从静态角度剖析了企业采用行为的一般机理用以说明为什么各企业在采用技术创新上有先有后，并建立了企业采用决策行为模型，定量刻画企业采用决策响应概率与动力结构的关系；其次从动态角度提出了企业状态演变概念并定义了两个状态变量，通过对两个状态变量的影响因素分析和动态分析，用以说明某一企业为什么和如何实现由未采用者到采用者的转变；第三对企业采用行为进行定量形式化构造了采用效果函数及选择概率函数等，并得到了调查结果的支持。

Uses fixed time the quota, little gives adds industriously feeds the method,"the quota" is fixed time advantageous in secretes the cow establishment conditioned reflexes, before picking the food to secrete the ration the digesting fluid, enhances the nutrients the true nutritive value:"little gives adds industriously" may maintain in the rumen the environment stability, causes the food broom corn millet evenly through the digestive tract, enhances the feed the true nutritive value and the absorption rate.

采用定时定量，少给勤添的饲喂方法，&定时定量&有利于泌乳牛建立条件反射作用，在采食前分泌一定量的消化液，提高营养物质的消化率：&少给勤添&可以保持瘤胃内环境的稳定，使食糜均匀通过消化道，提高饲料的消化率和吸收率。

It is an important route to increase efficiency and reduce pollutants on coal using for resident that clean coal technologies suitable for resident is to be chosen and used. In this paper, a quantitative assessing method on clean coal technologies us is used to assess and screen clean coal technologies suitable for resident . Integrated cost for unit energy production on various clean coal technologies suitable for resident is coal cleaning 48.37 yuan/GJ,coal molding for resident 45.37 yuan /GJ,coking 28.53 yuan/GJ,coal pressurized gasification combined with methanol 54.73 yuan /GJ,coal pressurized gasification combined with oil and 50.71 yuan/GJ,coal two-segment stove gasification 88.66 yuan/GJ,erection stove gasification 38.84 yuan /GJ. Integrated cost for unit energy production on coking is the least.

选用合适的民用清洁煤技术是提高居民生活用煤能源利用效率、减少环境污染的重要途径之一，本文利用笔者提出的清洁煤技术定量评价方法对民用清洁煤技术进行了定量评价与筛选，评价结果表明，在标准状态下各种民用适用清洁煤技术的单位综合成本分别为选煤48元/GJ，民用型煤45.73元/GJ，炼焦制气28.53元/GJ，加压气化联产甲醇54.73元/GJ，加压气化联产油蜡50.71元/GJ，两段炉气化88.66元/GJ，直立炉气化38.84元/GJ，其中炼焦制气是单位综合成本最小的民用清洁煤技术。

This article proposed a new method of coal virtual enterprise copartners selection,The method first obtains from the coal blending angle.

提出了煤炭虚拟企业合作伙伴选择的新方法，方法首先从配煤的角度入手，根据虚拟企业目标，把虚拟企业目标所要求的一些定量指标通过线性规划的方法进行定量分析，准确地确定能够满足虚拟企业目标定量指标的一些企业。

The relative satisfactory results of evaluating the peak height could be achieved by using the graphical measures established on this appropriate baseline in the case of different dilations or resolutions, degrees of separation and parameters of the peak.

基于小波变换是线性变换，提出了一种小波变换用于双组分重叠方波伏安信号定量分析的新的基线选取方法，(1)通过该基线建立的定量测量方法在不同尺度或不同分解次数，不同分离度以及不同峰参数的影响下，均可得到比较满意的峰高定量结果。

Expression analysis with RT-PCR revealed that PcLGBP gene was exclusively expressed in hepatopancreas and the expression could be up-regulated by heat-killed Listonella anguillarum. The binding specificity of PcLGBP to ligand were subsequently examined by recombinant expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3) and ELISA approach.

为分析PcLGBP基因的功能，通过半定量RT-PCR方法研究了其在组织中的分布，并用荧光定量PCR技术分析了其对细菌刺激的响应，结果表明PcLGBP基因是肝胰腺特异表达的基因，能响应热致死鳗弧菌刺激而增强表达，说明PcLGBP基因可能参与螯虾的细菌防御反应。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。