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Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上,根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点,设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针,优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件,建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法,组装了牛、羊、猪种布鲁氏菌检测试剂盒,该方法能特异检测上述3个种的布鲁氏菌。

According to these computated results, the correct experiment values of electron affinity can be chosen from the values of electron affinity which are measured by various experimental methods.

通过计算说明了电子亲合力与诱导效应的定量相关比电负性与诱导效应的定量相关好得多;并应用其定量关系式预测尚未实测到的取代基的电子亲合力值;根据计算结果,可从不同实验方法测得的电子亲合力值中选择正确的实验值。

PTA and FTD at all degrees and FTD at the 18 and 24 degrees of flection can be used to investigate the position of the patellofemoral joint effectively in kinematic MR and we suggest using the PTA, FTD and FTA at the 20 degree of flection in axial plane to study the patellofemoral joint in the future clinic.

动态MRI下的PTA及PTD可以很好的定量评价髌股关节的对合关系,FTD在18及24下也可以用来定量评价髌股关节的对合关系,建议在膝关节屈曲20左右进行MR检查测量PTA、FTD、FTA来定量评价髌股关节。

The present study explored the effects of morphine on plasticity of spinal cord in three parts as follows: 1. the effects of morphine on dorsal root terminal in spinal lamina Ⅱ of spared root rat---- a quantitative study by FRAP histochemistry; 2. the effects of morpnine on synaptic reinnervation in spinal lamine Ⅱ of spared root rat----an EM quantitative study and 3. the effects of morphine on collateral sprouting of nerve fiber in spared root of rat----an EM quantitative study

本课题从三个方面探讨了吗啡对脊髓可塑性的影响:1、吗啡对备用根大鼠脊髓Ⅱ板层背根终末的影响——抗氟化物酸性磷酸酶组化定量研究;2、吗啡对备用根大鼠脊髓Ⅱ板层突触重建的影响——电镜定量研究;3、吗啡对大鼠备用根神经纤维侧支出芽的影响——电镜定量研究。

The purpose of this study was to investigate the relationship between immune variables and psychopathology in patients with chronic schizophrenia before and after neuroleptic treatment, to evaluate the effect of neuroleptic on the immune parameters and the correlation between changes of immune variables and therapeutic effectiveness.

为探讨精神分裂症精神病理与免疫指标的相关性、评估抗精神病药对免疫指标的影响及其与疗效的关系,用固定剂量氟哌啶醇治疗50例慢性精神分裂症患者12周,在治疗前后测查T细胞亚群和白细胞介素2(IL-2)分泌细胞,并采用简明精神病评定量表、阳性症状评定量表和阴性症状评定量表进行评定。

Based on several studies concerned in the personality analysis and evaluation of nuclear power plant operators, a procedure and method of this research are identified and discussed to draw a fairly comprehensive picture of an operator's personality and to use personality data as predictors of a nuclear power plant operator performance.

基于国内外有关心理特质定量研究,提出了一套完整的程序和方法,来获取核电厂操纵员全面的心理特质定量数据及利用这些定量数据来评价操纵员的行为。

Pd could be quantificationally separated from Sb, Zrand Ce by flotation when the concentrations of potassium bromide and tetrabutyl ammonium bromide in solution were 1.5×10^(-2) molL^(-1) and 2.0×10^(-3) molL^(-1), respectively. The method was used for the separation and determination of Pdin the sample of synthetic water with satisfactory results.

当溶液中嗅化钾和四丁基溴化铵的浓度分别为1.5×10^(-2)molL^(-1)和2.0×10^(-3)molL^(-1)时,钯被定量浮选,实现了钯与锑、锆、和铈离子的定量分离,对合成水样中的钯进行定量浮选分离测定,结果满意。

Based on the molecular topological theory, a modified group dyeing indexL is introduced. It is used in the study of refractive index of the ketones and ethers. Relationship between the refractive index and molecular structure of 57 ketones and ethers is studied by using quantitative structure-property relationship technique. The reasonable molecular modeling equations are obtained by multiple regression for the prediction of the refractive index of ketones and ethers.

在分子拓扑理论的基础上,提出了一个修正的基团染色指数L,运用定量结构-性质相关技术研究了57种酮类和醚类化合物分子的折射率与分子结构间的定量关系;通过多元回归的方法建立了L与折射率的定量结构-性质相关模型。

Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法,定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数,同时将CK18作为淋巴结微转移的标志基因,定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

With the fast development of UCA, some contrast imaging method based microvessel perfusion assessment techniques have evolved from qualitative analysis to quantitative analysis.

然而基于&时间强度曲线&测量法的定量估计技术受仪器设置、成像方法等诸多因素的影响,因此目前还无统一的定量估计手段和定量标准。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。