定量溶液

Carbon nanotubes after oxidation and purification were used to pack a micro-column which was incorporated into a sequential injection system for facilitating online selective sorption of lysozyme from egg-white. The retained protein was afterwards quantitatively eluted by a carbonate buffer. With a sample loading volume of 2.0 mL, an enrichment factor of 12 was obtained, along with a retention efficiency of 100% and a recovery rate of 100%.

将多壁碳纳米管经氧化和提纯后装入固相萃取柱，在顺序注射系统中实现对蛋清中溶菌酶的在线选择性萃取，以碳酸盐缓冲溶液定量洗脱，进样量为2 mL时，富集倍率为12，吸附率和洗脱率均为100%，采样速率为10 h^(-1)，精密度为3.0%。

Coagulation by Aluminium Sulphate; this includes the tanks andpredetermined amount of Sulphates.

硫酸铝絮状物；包括储水罐和制备溶液及预先加定量硫酸盐的设备。

Methods: The protein and other impurity in the sample were precipitated by zinc acetate and potassium ferrocyanide after dipping of the sample. N,N-dichlorocyclohexylamine derived from sodium cyclamate by sodium hypochlorite in the filtrate was extracted by hexane. The HPLC separation was carried out with a phenomenex Gemini-C18 column using acetonitrile-water(7+3) or methanol-water (8+2) as mobile phase and detected by UV monitor at 314 nm. External standard method was used for quantification.

样品中甜蜜素浸提后，用乙酸锌和亚铁氰化钾试剂沉淀蛋白质等干扰杂质，过滤，用次氯酸钠将样品溶液中的甜蜜素转化为N，N-二氯环己胺经正己烷萃取后，在phenomenex Gemini-C18柱上，以乙腈－水(7+3)或甲醇－水(8+2)为流动相进行分离，HPLC-UV检测器在314 nm测定，外标法定量。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠，血清进行肝功能检测；苏木素－伊红染色部分肝组织；Trizol-酚－氯仿一步法提取小鼠肝组织总RNA，紫外分光光度法测定总RNA浓度及纯度，实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达，以18S基因作为质控。

Methods Streptomycin and dihydrostreptomycin were extracted from honey samples using a liquid extraction with a phosphate buffer followed by a solid-phase extraction based on weak cation-exchange columns. After sample preparation, the hydrophilic interaction chromatography was used to retain streptomycin and dihydrostreptomycin on the column for the separation of the analytes from endogenous compounds. The aminoglycosides were detected and quantified with electrospray ionization tandem mass spectrometry.

方法用磷酸盐缓冲溶液提取蜂蜜中的链霉素和双氢链霉素后，经弱阳离子交换柱进行固相萃取，样品制备后采用亲水作用色谱，使链霉素和双氢链霉素实现保留，从而与内源性物质达到分离，采用电喷雾离子化串联质谱法检测和定量。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。