定量溶液

After derivatization with 9-fluolenylmeghyl chloroformate at 40℃ in alkalescent environment (pH=8.0), taurine in samples was separated and quantified by HPLC.

在硼酸盐缓冲溶液存在的弱碱性(pH=8.0)环境中样品中的牛磺酸与9-氯甲酸芴甲酯在40℃水浴中反应生成衍生化产物，用反相高效液相色谱法检测定量。

It was quantitatively argued that the difference in the congruent melt compositions experimentally observed for the growth of compositionally homogeneous [00.1] and [10.0] -oriented LiNbO〓 single crystals could be a result from anisotropies in the surface free energy contributed to the total free energy of a crystal nucleus.

从热力学上定量阐明了沿不同晶体学方向生长LiNbO〓单晶时同成分熔化点成分之差别可能是由不同的晶体学固液界面能所致。研究结果表明双亚点阵离子溶液模型和化合物能量模型是处理硼酸盐溶体相和氧化物离子固溶体相的有效的热力学模型。

College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取，经碳酸钠溶液液-液分配净化，再用硅胶柱固相萃取净化，洗脱液均分成两份后经氮气吹干，分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生，采用选择离子监测模式进行测定，外标法定量。

Analysis on main points for determination of chlorine dioxide by iodometric method ;2. The experiment results show: To measure sulfide in wastewater by iodometric method may have errors because the interferent can not be removed.

用碘量法测定废水中的硫化物，可能因无法消除干扰而使测定结果存在较大误差；对于同一废水样品，当碘量法和亚甲基蓝分光光度法的测定结果存在显著性差异时，可用Pb2半定量法进行判定；对于某些化纤企业的废水，用氢氧化钾-乙醇溶液洗涤沉淀，可消除碘量法测定的干扰。

Image analysis system was used for semi-quantitive assay of TGF-βRⅠ and R Ⅱexpression, and microphotography was used to record the morphologic changes of culturedchondrocytes. The results are:1. TGF-〓 showed sequentially inhibitory and promotive effects on the proliferation of human articular chondrocytes of two week monolayer culture from one passage to eight passage.

本研究采用CellTiter 96〓单溶液细胞增殖分析试剂盒测定体外培养中的软骨细胞的增殖活细胞数量；免疫组化法测定软骨细胞TGF-βR Ⅰ、R Ⅱ，Ⅱ型胶原和S-100蛋白的定性表达；酶免分析法测定TGF-〓作用后的Ⅱ型胶原定量表达；通过图象分析仪，对TGF-βRⅠ、R Ⅱ的免疫组化染色结果进行半定量分析；本研究的实验结果如下：1。

Melamine in eggs was extracted and the protein in samples was precipitated with acetronitrile, melamine was separated on a Luna SCX column (Luna 5u SCX 250×4.6mm) with monopotassium phosphate(MKP 0.05mol/L, pH=3.00), acetronitrile buffer solution (70:30, by volume) as the mobile phase at flow rate 1.50mL/min and the detection wavelength was 240nm, quantitative with external standard method.

采用乙腈作为样品中蛋白质的沉淀剂和三聚氰胺提取剂，流动相为0.05mol/L(pH=3.00)的磷酸二氢钾乙腈缓冲溶液，检测波长为240nm，流速为1.50mL/min，SCX(Luna 5u SCX250×4.6mm)色谱柱进行分离，HPLC-UV检测，外标法定量。

Oil film thickness along the piston skirt in motored engine was measured, and the comparison with theory result was given.

最后在实验研究中，本文国内首次在活塞裙部油膜厚度中采用了激光荧光诱导的测试方法，主要工作是：分析了激光荧光诱导法的基本原理，建立了测膜厚的基本定量关系模型，通过实验分析了膜厚测试的各种影响因素，通过实验对不同香豆素荧光光谱和激发光谱特性进行了分析，通过实验确定了选取香豆素的种类和溶液浓度的基本原则，自主开发了以光纤为光学光路的测试系统并对系统进行了标定，对内燃机冷拖动状态的活塞裙部进行了实测，并与理论测试结果进行了对比。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

A method for cold transportation of goods that are placed in a hollow-walled refrigerating container, comprising the following steps: storage of a pumpable solution of tiny ice crystals, known as binary ice, having a given composition and temperature, in a binary ice store until said binary ice is used for refrigerating or filling purposes; the optionally filled wall of the refrigerating container that is to be used is emptied via an outlet valve for the binary ice; binary ice is allowed to circulate inside the refrigerating container via filling instruments that are supplied with binary ice from the binary ice store until the temperature of the wall drops to a given temperature, whereby the heated fluid that is discharged from the outlet valve is fed to a binary ice fluid store; the wall of the refrigerating container is filled with a given amount of binary ice; the refrigerating container is decoupled from the filling instruments; the binary fluid that result from the melted binary ice is returned to a corresponding binary fluid store in order to produce new binary ice from said binary fluid.

一种置于一中空壁制冷容器里的制品的冷运输用的方法包括下列诸步骤：将一种具有一预定的组成和温度的可被泵送的小的冰晶体的溶液保存在一个二元冰储槽中，直到所述的二元冰用于制冷或充填目的；通过一二元冰出口阀将待被使用的一制冷容器的随意被充填的壁排空；用二元冰充入该制冷容器的被排空的壁内；通过充填器具使该二元冰在该制冷容器里循环，这循环由来自二元冰储槽的二元冰供给，直到壁温下降到一预定值，从而从该出口阀放出的被加热的流体被提供给一个二元冰流体储槽；将一给定量的二元冰充入该制冷容器的壁里；从诸充填器具上拆离该制冷容器；将从溶化的二元冰形成的二元流体返回到一相应的二元流体储槽内，以便从所述的二元流体制取新的二元冰。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。