定量测定

Seaweed extract-EClean was applied to treatment for eutrophication water. Quantative detection of nitrogen cycle bacteria showed that seaweed extract could improve the growth of ammonifying bacteria, nitrosobacteria and nitrobacteria in the treated water. There was also an increasing trend in the number of denitrifying bacteria in aerated treatment water, which showed that the seaweed extract could accelerate nitrogen cycle and the release of nitrogen from water.

利用国外引进的海藻提取物EClean制剂对富营养化水体进行处理试验，经过定量测定处理和对照水体中氮循环细菌数量的变化，得出EClean制剂能够促进水体的氨化细菌、亚硝化细菌、硝化细菌的生长，并维持了曝气条件下一定数量的反硝化细菌，通过此处理使得在同一反应器中、同一条件下完成硝化作用和反硝化作用成为可能，加速了水体的氮素循环，使水体总氮下降明显。

Methods:The minimum inhibitory concentration of the olive polyphenol against the common oral pathogenic bacterium was quantitatively determined with the agar-agar dilution method combined with the micro liquid dilution method.

选用质量控制标准菌株和临床分离的对多数抗生素耐药的菌株，采用琼脂稀释法和微量液体稀释法相结合的药敏实验方法，定量测定橄榄多酚对口腔颌面部感染常见病原菌的最低抑制浓度。

An accurate new method has been established as an alternative of the test of nucleotide in transfer factor tablets.

本方法准确可靠，为转移因子制剂中核苷酸的定量测定提供了一种新的思路。

After collection and drying, the product is weighed on an analytical balance and, from the mass and known stoichiometry the original analyte is quantitatively determined.

收集产物并干燥后，用分析天平称重，从重量和已知的化学计量配比可以定量测定原始分析物。

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算，连续10d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算，连续10 d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The detection and quantification limits of scutellarin obtained were 0.28 mg/L and 0.93 mg/L, respectively.

野黄芩苷的质量浓度采用高效液相色谱进行离线测定，得到的检出限和定量测定下限分别为028 mg/L和093 mg/L，日内和日间精密度分别为1.7%和3.6%，平均加标回收率为98.3%。

For the determination of five carbamate pesticide residues by high performance liquid chromatography with gel permeation chromatography purification, sample was extracted with acetonitrile and purified with gel permeation chromatography. Methanol-water was used as the mobile phase by gradient elution, flow rate of 1.0mL/min, using a fluorescence detector and quantitating with external standard method.

凝胶渗透色谱-液相色谱法测定5种氨基甲酸酯类农药残留中，实验采用乙腈提取，凝胶渗透色谱柱对样品进行净化，以甲醇-水为流动相，进行梯度洗脱，流速1.0mL／min，荧光检测器检测，外标定量测定。

Objective: To establish a quantitative method for detecting simultaneously the cyclamate in preserved fruits.

目的：建立同时测定功能性饮料中牛磺酸和赖氨酸的定量测定方法。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了