定量测定

After 1,4, 7, 10, 14 days of exposure, eight rats of each group were sacrified to assess lung histopathologic changes.

两组分别于暴露满1、4、7、10和14d时，每组取动物8只，行肺组织病理学检查，半定量逆转录聚合酶链反应测定COXI、COXII和COXIIImRNA的表达。

Quantitative RT-PCR and hybridization methods were used to deter mine the effect of inhibition of HCV by ribozymes.

CD-SRα中，转染HCV感染的MT-2细胞，用定量逆转录-聚合酶链反应、核酸杂交等方法测定核酶对HCV的抑制作用。

Quantitative RT-PCR and hybridization methods were used to deter mine the effect of inhibition of HCV by ribozymes.

CD-SRα中，转染HCV 检查感染的MT-2细胞营养，用定量逆转录-聚合酶链反应、核酸杂交等方法测定核酶对HCV的抑制作用。

Quantitative polymerase chain reaction test technique and its clinical application.

定量聚合酶链反应测定技术及其临床应用。

Methods: The protein and other impurity in the sample were precipitated by zinc acetate and potassium ferrocyanide after dipping of the sample. N,N-dichlorocyclohexylamine derived from sodium cyclamate by sodium hypochlorite in the filtrate was extracted by hexane. The HPLC separation was carried out with a phenomenex Gemini-C18 column using acetonitrile-water(7+3) or methanol-water (8+2) as mobile phase and detected by UV monitor at 314 nm. External standard method was used for quantification.

样品中甜蜜素浸提后，用乙酸锌和亚铁氰化钾试剂沉淀蛋白质等干扰杂质，过滤，用次氯酸钠将样品溶液中的甜蜜素转化为N，N-二氯环己胺经正己烷萃取后，在phenomenex Gemini-C18柱上，以乙腈－水(7+3)或甲醇－水(8+2)为流动相进行分离，HPLC-UV检测器在314 nm测定，外标法定量。

In this paper, we introduce the function of the confocal laser scanning microscopy in the studies of protein crystal growth kinetics, such as measuring the numbers of crystal, reconstruction 3-D image to acquire the information of crystal growth process.

本文介绍了其在定量测量晶体的个数，重组三维图像以获得晶体生长的过程信息及测定晶体生长台阶动态变化等方面的应用。还对激光扫描共聚焦显微镜在晶体生长研究的其它方面应用前景作了展望。

Objective To investigate bone nutritional status of neonates and their mothers as well as the correlation between them by estimating the concentrations of 25-D3, calcium and phosphorus in maternal blood and cord blood at birth, and by measuring the bone speed of sound of neonates and their mothers with quantitative ultrasound within 3 days after birth. Methods The concentrations of 25-D3, calcium and phosphorus in the serum were estimated both from 32 pregnant women who had a term delivery and from the umbilical cord at birth.

摘 要：目的通过对产妇及其新生儿在分娩当天进行血25-D3、血钙和血磷浓度的测定以及在产后3 d内采用骨定量超声仪对母儿进行骨超声波速度(speed of sound，SOS)的测量来评价母儿的骨营养状况和两者的相关性。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠，血清进行肝功能检测；苏木素－伊红染色部分肝组织；Trizol-酚－氯仿一步法提取小鼠肝组织总RNA，紫外分光光度法测定总RNA浓度及纯度，实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达，以18S基因作为质控。

The standard sample of high pure silicon tetrachloride with certified value of 99.99 % was analyzed. The obtained average determination result was 99.857% after normalization treatment. The relative standard deviation was 0.03%.

对认定值为99.99%高纯四氯化硅标准样品分析，归一化法定量，得到该样品中四氯化硅的测定平均值为99.857%，相对标准偏差为0.03%。

Litopenaeus vannamei were continuously fed by basic diets containing three levels (1%, 0.5%, 0.1%) Ig-Guard respectively for 20 days, and the basic diets as blank control. Phenoloxidase, acid phosphatase, alkaline phosphatase, lysozyme activity of the serum and superoxide dismutase, catalase activity of the muscle, and protein concentration of the serum and the muscle were detected in the 5, 10, 15, 20 day.

采用连续投喂的方法，饲喂凡纳滨对虾 20 d，分别测定了第5，10，15，20 d血淋巴的酚氧化酶、溶菌酶、酸性磷酸酶、碱性磷酸酶及肌肉匀浆液的超氧化物歧化酶、过氧化氢酶等非特异性免疫因子活性，并对血清及肌肉匀浆液中蛋白进行定量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。