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Bis (P—bromobenzylamino) ethane and 1,2—bis (m—Iodobenzylamino) ethane may be prepared by condensation of the corresponding halo benzaldehyde with ethylenediamine and followed by catalytic hydrogenation of the Schiff's bases over Pto_2 in glacial acetic acid solutions: Both Ⅱa and Ⅱb may react in neutral mediam with aldehydes to form well—defined crystalline tetrahydro—imidazole, which may be served as derivatives of identification for aldehydes: Under the same condition, however,Ⅱb may ...

用1,2-二乙烷与二十余种醛类作用,得到有固定熔点的晶体衍生物,它们的熔点分布在85—210℃之间,产率为40—96%,这试剂与甲基酮类在相同条件下无反应,因此,它可作为鉴定醛类的专属试剂。 2。上述衍生物用稀盐酸在室溫处理,能发生水解,可以定量地收回原来的醛,因此这试剂又可作为分离醛类的试剂。 3。上述衍生物可以用非水滴定法半微量地测定其分子量可作为鉴定醛类的另一项参考数据。 4.1,2-二乙烷与醛类能生成晶体衍生物,但这试剂也与甲基酮在同样条件下发生反应,所以不适合作为鉴定醛类的专属试剂。

The susceptible strain, the abamectin resistant strain and the high temperature tolerant strain from Tetranychus cinnabarinus were used to study cross tolerance to abamectin and high temperature using the methods of bioassay and fluorescent real time quantitative PCR.

以朱砂叶螨敏感种群、抗阿维菌素种群和耐高温种群为材料,应用生物测定方法以及荧光实时定量PCR技术,研究朱砂叶螨抗药性和耐高温间存在的交互耐性及机制。

The power-time curves of E.coli reacting with Co-SG at different temperature has been determined by microcalorimtric method The multiplication rate constant k, generation time G,bacterial growth inhibition ratio I,total thermogenetic quantity Q, the heat quantity of a single bacterium Q_(0) and the heat quantity of a single bacterium per minute have also been calculated according to the power-time curves.

用微量热法测定了大肠杆菌及其在Co -SG配合物作用下的热谱曲线,计算了速率常数k ,传代时间G、抑制I、总产热量Q、单个细菌的产热量Q0 和每分钟单个细菌的产热量 Q0 ,建立了部分代谢参数之间的关系,探讨了大肠杆菌在不同温度和Co -SG作用下的代谢抑制,发现可用t、tr、tg和 Q0 定量表征在不同温度下大肠杆菌的生长代谢和药物的抗菌活性。

Principle— The titrimetric determination of water is based upon the quantitative reaction of water with an anhydrous solution of sulfur dioxide and iodine in the presence of a buffer that reacts with hydrogen ions.

原理---水分滴定测定法是基于水与二氧化硫和碘的无水溶液,在能与氢离子反应的缓冲溶液下发生的定量反应。

Methods:To make model of kidney yang deficiency by injecting acetic acid cortisone in rats.It had Detected calmodulin mRNA expression level of hypothalamus-pituitary-adrenal axis in kidney yang deficiency rat and the effect of kidney tonify herbs Yougui Yin for it.

用醋酸可的松造成肾阳虚模型,以逆转录多聚酶链反应半定量法,测定肾阳虚大鼠下丘脑-垂体-肾上腺轴CaMmRNA的表达,以及补肾中药右归饮对其的影响。

Ammonifying rate, nitrosifying rate,nitrifying rate and denitrifying rate were determined by measurement of conversion efficiency and accumulating rate of nitrogenous matter by ammonifying bacteria, nitrobacteria, nitrifying bacteria and denitrifying bacteria in the surface 0-1.5 cm of pond sediment from the intensive fish culture ponds.

通过对精养鱼池表层淤泥中氨化细菌、亚硝化细菌、硝化细菌和反硝化细菌对含氮物质转化率的测定,定量了解了上述细菌的氨化率、亚硝化率、硝化率和反硝化率。

MMP3, TIMP-1 expression of the rats myocardia are measured with RT-PCR.

采用半定量逆转录聚合酶链反应的方法测定大鼠心肌组织MMP-3、TIMP-1的表达。

According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度,按载体定量杀菌试验,将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下,各取洗脱液50μl,接种于TSA平板并置厌氧箱(90%N2,10%CO2,37℃)培养24-48h后,进行活菌菌落计数。

The combined extract was concentrated to dryness and redissolved in acetonitrile, then cleaned up by dispersive solid-phase extraction with sorbents of N-propyl ethylene diamine, graphited carbon black, and C18, and determined and confirmed by GC-NCI/MS.

样品经乙腈提取并浓缩后加入N-丙基乙二胺、石墨化碳黑和C183种填料进行分散固相萃取净化,气相色谱-负化学离子源质谱分时段选择离子监测技术测定与确证,外标法定量。

Based on the conclusion of determining the concentration of rChIFN-γprokaryotic expression and titrating antiviral of sf9 cells, to establish the quantitive ELISA for ChIFN-γ.

在测定原核表达rChIFN-γ的浓度和滴定昆虫细胞表达rChIFN-γ的抗病毒的活性的基础上,建立了鸡γ-干扰素的定量ELISA检测方法。

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