定量测定

Of conclusion leukaemia patient outside configuration of week blood white blood cell and structural change can pass VCS parameter to determine mensurably, if partial parameter is neuter distributinging breadth of bead cell volume, lymphocyte is average bulk is leukaemia the sieve is checked, diagnosed new index.

结论白血病患者的外周血白细胞形态和结构变化可以通过VCS参数定量地测定，部分参数如中性粒细胞体积分布宽度、淋巴细胞平均体积是白血病筛查、诊断的新指标。

The feasibility of the equipment to measure Tg of amorphous powder is verified with a lot of experimentl results.The Tgs of some typical starches such as corn, pachyrhizus, buckwheat and pea in different water content are obtained by TDA. It is found that the water has plastic effect on starch and the Tg of starch decreases with the increase of water content. Through this study a quantitative expression is established to relate Tg and water content, and it is testified with experimental results for the sticky rice starch with different water content.

采用热膨胀法测定不同含水量下玉米、地瓜、荞麦、豌豆淀粉的玻璃化转变温度，得到了随着含水量的增加Tg降低的关系，探讨了水的增塑作用及影响机理，得到含水量与Tg的定量表达式，并用糯米淀粉的实验值对该关联式的有效性进行了验证，为预测不同含水量的淀粉的玻璃化转变温度及对淀粉的储藏加工提供了理论依据。

The other model of focal cortical ischemia in Wistar rats was induced photochemically, regional cortical blood flow, the content of polyamine in regional cortical homogenate, and the quantitative parameters of neuronal mitochondrial lesion were also measured.

本研究还对光化学诱导鼠脑局灶缺血模型，进行了脑皮质灌流量、脑组织多胺含量及线粒体病理的定量指标的同期观察与测定。

Applying the theory of deformation partitioning to this area, the author discuss the relationship between deformation and porphyroblast growth, and get the conclusion of the sequence of deformation and metamorphism. At the first time we measure the distribution of chemical composition within the porphyroblast by XRF and confirm the theory of deformation partitioning quantitative.

利用宝音图群泥质变质岩中的存在大量变斑晶矿物，应用变形分解理论，讨论了变斑晶生长和变形关系，确定了变质变形演化序列，提出了变斑晶的生长相，并首次利用同步辐射XRF技术测定了变斑晶矿物的化学成分分布，主要是微区的微量元素成分分布，定量地证实了变斑晶矿物生长相的存在，进而讨论了与变形关系，并进行了变斑晶矿物不同生长相的温压计算。

Methods Seventy-four strains of Salmonella enterica serovar enteritidis (S. enteritidis), S. pullorum and S, gallinarum isolated from chickens were determined for biofilm using crystal violet staining, and the strain C050041 with well biofilm formation was chosen to construct a mutant library using transposon mutagenesis.

利用结晶紫染色定量法对74株鸡源的肠炎、鸡白痢和鸡伤寒沙门氏菌进行生物膜测定，选择生物膜生长较好的肠炎沙门氏菌C050041，采用转座子随机插入法构建突变株库。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half????

采用萘胺重氮分光光度法测定血清NO含量，采用半定量RT？？？？

Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half quantitative reverse transcription polymerase chain reaction was used to detect eNOS gene expression in artery vessels.

采用萘胺重氮分光光度法测定血清NO含量，采用半定量RT PCR方法检测各组动物动脉壁匀浆中eNOS基因的表达，分析造模及各药物组血清NO含量及eNOS基因表达的变化。

Analysis the changes of each groups mRNA expression of ICAM-1,VCAM-1 and eNOS .Naphthylamide Heavy nitrogen spectrophotography was adopted to detect thecontent of nitric oxide in the blood serum. To try to find out the mechanism of QDTMT prevent and cure function on AS.

采用蔡胺重氮分光光度法测定血清中NO含量，采用半定量RT一PCR的方法分别检测各组动物动脉壁匀浆及单个核细胞中ICAM一l、VCAM一1 mRNA的表达，以及动脉壁匀浆中eNOSmRNA表达，并分析造模及各药物组上述指标mRNA表达的变化；进一步探讨QDTMT防治As作用的机制。

Methods For the mild solubility of chloroform and tetrachloromethane in drinking water, chloroform and tetrachloromethane were analysed by static headspace gas chromatography with DB-5, Rtx-1 and DB-1 capillary columns and electron capture detector, external standard method was used for quantification.

利用氯仿和四氯化碳在水中的弱溶解性，采用静止顶空气相色谱法进行分析，使用DB-5、Rtx-1和DB-1毛细管柱和电子捕获监测器测定，外标法定量。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了