定量测定

Real-time quantitative pcr, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of icam-1, the change of mrna at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用marmarou方法获得大鼠弥漫性脑损伤模型，实时定量rt-pcr、s-p免疫组化法分别测定icam-1蛋白和mrna在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

Methods The rat models of diffuse brain injury were established by Marmarou's method. Real-time quantitative PCR, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of ICAM-1, the change of mRNA at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用Marmarou方法获得大鼠弥漫性脑损伤模型，实时定量RT-PCR、S-P免疫组化法分别测定ICAM-1蛋白和mRNA在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取，经碳酸钠溶液液-液分配净化，再用硅胶柱固相萃取净化，洗脱液均分成两份后经氮气吹干，分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生，采用选择离子监测模式进行测定，外标法定量。

After stenting, BP and number of antihypertensive medications were significantly improved in patients with a hyperemic systolic gradient HSG .21 mm Hg vs.

压力导丝测定的HSG在选择患者方面优于 IVUS和定量冠状动脉造影。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

By using white copper alloy tablet to determine Ni - Cu- Zn X - ray fluorescence instensity qualitively, and doing intensitive normalization may semi - quantitatively analyse major components percent, the results have shown that there is obvious absorption and enhancement effect.

直接用白铜合金片样定性扫描测定Ni、Cu和Zn的X-射线荧光强度值，作强度归一化可以半定量确定主量元素质量百分数，结果发现元素之间有明显的吸收—增强效应。4 Th normalization

In this study, we evaluated the effect of Isatis indigotica radix extract in experimental murine model against pulmonary fibrosis.

经由肺脏检体的组织切片染色后，可以用半定量型态学分析来判断肺脏的发炎情形；经过判读后，结果和测定出来的生化值有相同的趋势，在投予板蓝根甲醇粗抽物后，减轻了肺脏的发炎程度。

It is introduced briefly the modification way and result of lignosulphonate as coal-water slurry additive and determination of the adsorption film thicknesses of the LS and modified lignosulphonate on the smooth surface of coal and microscopic slide by the method of elliptical polarization .

简要介绍了木质素磺酸钠用作水煤浆添加剂的改性方法及结果，并采用椭圆偏振法测定了木钠及改性木钠在光滑的煤表面和载波片表面的吸附膜厚度，定量地对比了添加剂在固体表面吸附的空间位阻作用的大小。

Appraised the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis, according to the dynamic observation on integrative scores of lumbodorsal pain and lumbodynia,"VAS section grading method" on lumbodynia,"Barthel index rating method" on ADL, detected BMD on anterior-posterior position lumbar2~4 vertebra body and proximate of femur including neck/ward/troch, and determined the index of bone transition including BGP/PINP/ICTP through the radioimmunity method before and after treated half a year, then contrast intragroup and selfgroup. Depending on the standard of curative effect, contrast the difference of curative effect between two groups.

采用随机对照的方法，分为治疗组36例，对照组34例，依据对腰背痛及腰痛综合评分进行动态观察；依据&疼痛VAS线段分级法&对腰背痛（静止痛、翻身痛、久坐痛、起身痛、弯腰痛、步行痛、睡眠痛、叩击痛、压痛）进行量化动态观察；依据&Barthel指数评定法&对老年性骨质疏松症日常生活活动能力进行观察；采用双能X线骨密度仪对腰椎(L2-L4)、股骨近端各部位：股骨颈、股骨三角区、股骨粗隆进行骨密度定量检测；应用放射免疫法对骨转换指标进行测定，骨形成指标：骨钙素、Ⅰ型前胶原氨基端前肽；骨吸收指标：Ⅰ型胶原交联羧基末端肽等，评价骨碎补总黄酮治疗老年性骨质疏松症的临床效果。

Determine you medicine effective composition in the Chinese ephedra with HPLC (regard content that the hydrochloric acid of Mahuang are counted ) as finished product index that ration control.

以HPLC法测定君药麻黄中有效成分的含量作为成品定量控制的指标。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。