定量测定

To explore the characters of the emotional changes of religious experience and the relationship between religious experience and physiological indicators, we used self note with different pictures to induce emotion, and used the emendatory Plutchik cinque mood scales assess the subjective experiences, and used the polygraph record the physiologic measures and EEG data while the subjects were watching the pictures, and took our experiment on 18 Christians and non Christians, in which there are 9 Christians usually experience the religious experience and 9 have no faith, and all of them are college students.

为了探讨宗教体验的情绪变化特征及其与生理活动的相关，研究采用自编的图片诱发情绪，用修订的Plutchik五分制情绪心境评定量表测定主观情绪体验，用多导生理仪记录被试在观看图片时的各项生理指标及脑电指标，对18名基督徒和非没有任何信仰的被试进行了实验。其中有基督教信仰且都经常会有宗教体验和没有任何信仰的被试各9名，所有被试均为大学生。

Determination of ethyne in liquid oxygen or liquid air by GC was introduced in this paper,where GDX-501 was used as fixed phase.

采用GDX-501为固定相，用保留值和加入已知物增加峰高法进行定性，用外标法进行定量，建立了气相色谱法测定液氧、液空中微量乙炔含量的方法。

Methods The levels of MIF protein and mRNA in PBMC from 64 AD patients and 50 healthy subjects were measured using ELISA and Fluorogenic quantitative PCR, respectively.

方法应用ELISA法和荧光定量PCR技术，测定64例AD患者和50名健康对照者外周血MIF的表达水平。

MethodsThe frequency of CD4+CD25+T cells in the peripheral blood mononuclear cells from 30 cases of severe hepatitis B, 20 cases of chronic hepatitis B, 20 cases of chronic asymptomatic HBV carriers and 10 healthy controls were determined by flow cytometry and their serum HBV DNA levels were detected by Quantitative Fluorogenic PCR.

方法采用流式细胞仪检测30例重型乙型肝炎患者、20例慢性乙型肝炎患者、20例无症状乙肝病毒携带者和10例健康的外周血CD4+CD25+调节性T细胞（CD4+CD25+Treg）的水平，并应用荧光定量PCR方法测定上述研究对象血中HBV DNA滴度。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Quantitative germicidal test method and chemical measuring method were used to carry out laboratory observation.

方法采用定量杀菌试验方法和化学测定方法在实验室进行了观察。

Gibel carp were inoculated with formalin killed Aeromonas hydrophilaand fed with diets supplemented with appropriate amount of yeast β-glucan. 28 days after the inoculation, the infulence of dietary yeast β-glucan on the resistance of gibel carp against Aeromonas hydrophila were determined by examing their gained weight, agglutinating antibody titer, lysozyme activity, phagocytic activity and relative percent survival by challenging with live A.

在饲料中添加定量的酵母β-葡聚糖，投喂经注射接种福尔马林灭活的嗜水气单胞菌(Aeromonas hy-drophila)菌苗的异育银鲫28 d后，通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率，探讨了酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Glyoxal in cosmetic sample was extracted by the solution of acetonitrile and water.

样品用乙腈－水溶液溶解，与2，4-二硝基苯肼在弱酸条件下衍生化反应，衍生物直接用HPLC测定，用外标法定量。

Methods Peripheral blood neutrophils were islolated from health men, and then cultured in DMEM medium containing 10% fetal calf serum for 3 h. Blank medium or gonococcal inocula was added into group A or group B. NO concentration and iNOS mRNA expression at 0, 3, 8 and 12 h were determined by cadmium reduction method and realtime quantitative fluorescent PCR, respectively.

分离正常人外周血中性粒细胞，在10%胎牛血清DMEM培养液上培养3 h，A组加入空白培养基，B组加入淋球菌菌液，随后在0、3、8、12 h分别以实时定量荧光PCR测定iNOS mRNA表达，用镉还原法检测NO浓度。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。