定量测定

We would like to make our students to know and master the basic way of the chemistry experiments, the use of the normal apparatus of inorganic and analytical chemistry, the character of the normal inorganic compounds and

课程的目标是使学生了解和掌握化学实验的基本方法和常见的无机与分析化学仪器的使用，了解和掌握一般无机化合物的性质以及分离提纯的方法；熟悉常见的无机化合物的制备方法，掌握测定有关重要化学常数的方法和原理；熟悉滴定分析的基本操作，掌握定量测定无机化合物的主要化学滴定的方法和原理，从而培养学生能用化学观点与方法来观察分析和解决实际问题的能力。

It is very significant and positive to search rapid methods of determination of nucleic acid.

核酸的定量测定是研究核酸的基础，目前核酸的测定方法有吸光光度法[2]、紫外光度法[3]、荧光分析法[4]和化学发光法[5]等。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

Chapter 4 gives a micellar electrokinetic chromatography method for the quantitative analysis of seven podophyllum lignans in podophyllum emodi wall.

第四章：建立了胶束电动毛细管色谱法分离测定抗癌药物桃儿七中七种主要有效成分的新方法；利用二阶导数电泳图定量测定低含量和在实际样品没有达到基线分离的组份。

The two components were measured by excitation-emission matrix fluospectroscopy both in simulated and real system. The results of measured data, which were resolved by alternating trilinear decomposition method . It was shown that it was feasible and simple method in the determination of two main components in the complex cortex fraxini without pretreatment and separation.

利用三维荧光进行测定，然后利用交替三线性分解算法对该数据进行分解，模拟样本和实际样本分辨和回归结果表明，在秦皮中其它荧光背景干扰共存下，可实现对秦皮萃取液中两种主要成分秦皮甲素和秦皮乙素的同时分辨和定量测定。

Locomotor activity was measured with amonitor and rCMRglc were measured with the quantitative autoradiographic[14C]2-deoxyglucose procedure in 62 brain regions of Fischer-344rats at 2, 30, 60, 120, and 180 min after IV administrationof saline or midazolam 5 mg/kg.

在静脉注射盐水或咪达唑仑（5 mg/kg ）后2，30，60，120和180min 后测定 T-344大鼠62个脑区的自主运动活性及 rCMRglc （用2 -去氧葡萄糖（14C ）放射性定量测定法）。

Was studied by Folin Ciocalteu colorimetry. The results showed that polyphenol content of C. album could be well calculated according to their colorime tric absorption at 765nm by applying Folin Ciocalteu reagent 1.5mL and 10% Na2CO3 6 mL,2 h at 30℃ for colorimetric reaction, and the linear range of standard curve was 7-70mg/L.The method established was very convenient, stable and accurate.Key words: Canarium album Rauesch.;polyphenol;colorimetry

结果表明：橄榄多酚提取液在加入Folin Ciocalteu试剂1.5 mL，10% Na2CO3 6 mL，反应时间2 h，温度30℃的条件下进行显色，并于765 nm处测定其吸光值，多酚质量浓度在7～70mg/L范围内与吸光值有良好的线性关系，根据此线性回归方程进行橄榄多酚的定量测定，该方法简单方便，稳定性好，准确度高。

A spectrophotometry method, which based on the reaction of diacetyl monoxime and urea in acidic solution, was established for determination urea in bovine milk.

根据二乙酰一肟与在酸性条件下尿素的反应，建立了一种分光光度法快速测定牛奶中尿素含量的方法，该方法可以定性和定量测定牛奶中尿素含量。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了