定量测定

Quantitatively: Reciprocal of the value of the angular separation of two neighboring objects which the eye can just perceive as being separate.

定量方面：任一空间分辨力的测定结果，例如观测者恰可察觉分离的两个相邻物体的用弧分表示的角度间隔的倒数。

The methods is simple, accurate, reproducible and can be used for the quality control of Flos Forsythiae.

定量方法简便、准确，重现性好，可用于连翘花中连翘苷的含量测定。

Laminas of vertebra were restrictedly excised.After excitability of posterior root of spinal nerve was excited,lumbar spinal nerve were cut off.Results:The convulsion of low limb was dispelled in the 194 patients.

手术采取限制性椎板切除，神经阈值测定仪测量腰脊神经后根的兴奋性后，行腰脊神经后根的高选择性定量切断。

Among these techniques, Northern blot, ribonuclease protection assay, real-time quantitative polymerase chain reaction, which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法，即Northern印迹、RNase保护试验和实时定量PCR技术，特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

Objective To establish the qualitative study methods for Sinapine thiocyanate in Raphanus sativus L.Methods The contents of Sinapine thiocyanate in Raphanus stativus L.was determined by HPLC.

目的对莱菔子中芥子碱硫氰酸盐进行定量研究方法采用高效液相色谱法对莱菔子中芥子碱硫氰酸盐进行含量测定。

E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods The propofol in blood was extracted with cyclohexane,and thymol was used as the internal standard,determining the concentration of propofol by HPLC fluorescence detector.

用环己烷提取全血中的丙泊酚，以百里酚为内标定量，用HPLC 荧光检测器测定丙泊酚含量。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Methods SYBR Green I real-time polymerase chain reaction was used to detect the expression of VEGF mRNA in articular cartilage from 20 OA patients and 10 controls with traumatism. VEGF protein was detected by enzyme-linked immunosorbent assay in articular cartilage.

应用SYBR Green Ⅰ实时定量聚合酶链反应检测20例OA和10例外伤患者关节软骨中VEGF mRNA表达，并用酶联免疫吸附试验测定关节软骨组织VEGF含量。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。