定量测定

MTT colorimetric assay was used to measure the proliferation of VSMCs. The expression of intranuclear proto-oncogenes c-myc and proliferating cell nuclear antigen were observed by immunocytochemical stain and imageanalysis technique.

采用定磷法分别测定各组细胞CaM和CaN活性，MTT法检测细胞增殖活度的变化，免疫组化定量技术观察细胞内c-myc、PCNA的表达水平。

Methods The model of intravasation migration of cancer cell in vitro was used. The fibrosarcoma cell HT1080 was labelled with fluorescence, and the fluorescence guantities in 24-pore plate through collagen membrane and HUVEC single cells layer were detected in different time to eveluate the intravasation migration of cancer cell.

利用体外癌细胞向血管内游走模型，将纤维肉瘤细胞HT1080经过荧光标记，通过测定不同时间穿过胶原层及HUVEC单细胞层落到24孔板内的荧光量来定量评价瘤细胞血管内的游走情况。

Objective:Analysis of screening results of three neonatal diseases to further clarify the incidence of phenyl ketonuria,congenital hypothyroidism and glucose-6-phosphate dehydrogenase(G6PD) deficiency in Hainan Province.

目的：对海南省新生儿疾病筛查情况进行分析，了解海南省新生儿苯丙酮尿症、先天性甲状腺功能低下症、葡萄糖6-磷酸脱氢酶缺乏症(G-6-PDD)的发病率。方法：对出生72h后的7412名新生儿采集足跟血制作血滤纸干标本，PKU筛查采用荧光定量法检测滤纸干血斑中Phe含量；CH筛查采用时间分辨荧光免疫法检测滤干血斑中TSH含量；G6PD缺乏的筛查采用荧光斑点定性试验测定G6PD活性。

In addition, based on the 153 experimental A, B values and 56 predicted A, B values, the Koa values of the remaining 190 PCBs were predicted.

此外，根据实验测定的153个PCBs的GC保留参数以及定量构效关系研究中的56个预测值，对剩余190种PCBs的Koa值进行了预测。

It is by traditional Chinese medicine in beast it is serial in pharmaceutical for development and employing being of products in particle in in veterinarian theoretical foundation, Combine whom modern traditional Chinese medicine develop use mainly treatment , poultry of epidemic disease for , innovation its order , lie in series of products this belong to pure traditional Chinese medicine particle, not so incomplete as medicines, curative effect is precise ; Employ modern detection means to determine the effective composition, improve the production technology, there is no quantitative current situation to makeover traditional Chinese medicine, it is controllable to reach quality , and produce industrialization by animal remedy GMP management.

项目简介：兽用中药颗粒剂系列产品的开发及应用是在中兽医理论基础，结合现代中药开发的主要用于禽类疫病的治疗，其创新点在于该系列产品属纯中药颗粒，无药残，疗效确切；并应用现代检测手段测定有效成分，改进生产工艺，彻底改变中药无定量的现状，达到质量可控，并以兽药GMP管理进行产业化生产。

The microstructures and phases of the alloys were analyzed by OM, SEM and XRD; the erosion resistance of the alloys prepared by different processing were measured under the conditions of simulated-brine and salt atmosphere, besides the various corrosive rates were calculated by the method of weight lost; the lows of corrosion were analyzed by the electrochemical polarization curve, and the corrosive current was measured through the method of Tafel linear extrapolation.

用光镜、扫描电镜、X射线衍射分析了合金的组织与相的组成，测试了模拟海水及盐雾条件下三种工艺制备的材料的耐腐蚀性能，并用失重法定量计算各自的腐蚀速率；利用电化学极化曲线法分析了三种工艺制备的材料腐蚀规律，用塔菲尔直线外推法测定腐蚀电流。

The box-counting fractal dimension and information fractal dimension of beef marbling were estimated in order to find out a new parameter for the quantitative description of the distribution of marbling fat.

通过对牛肉大理石花纹图像分形特征的分析及分形维数的测定，试图以分形维对牛肉大理石花纹的分布特征进行定量描述。

Based on the method of BCA, we menstruate the molar extinction coefficient of R-PE. The molar extinction coefficient in the wavelength of 565nm and 280nm is 4.75X K^mor'cm"1 and 4.07 X104morIcm"1 respectively.

在BCA法进行蛋白质定量的基础上，测定了R-PE的565nm和280nm的摩尔消光系数，它们分别为4.75×10~5mol~(-1)cm~(-1)和4.07×10~4mol~(-1)cm~(-1)。

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

Kkay糖尿病鼠随机分3组：糖尿病组、高蛋氨酸组和叶酸、维生素B12组，C57BL/6鼠为对照(C57)，以相应饲料喂养4个月后取材，测定血糖、血同型半胱氨酸、叶酸和维生素B12，以PAS、六氨银染色法光镜下及超微电镜观察各组DN病变，比较各组肾小球硬化指数；以免疫组化和半定量图像分析肾小球MMP-9蛋白表达，并以RT-PCR检测肾脏MMP-9 mRNA水平。

Has established the Avey rhzomorph through the field test remains the analysis method in the sea cabbage and the soil, and remained to it in the sea cabbage and the soil has carried on the determination, the sample with the methyl cyanide extraction, after extracting the revolving concentration nearly did, with the few methyl cyanides dissolves, passed through PRS the solid phase extract column to purify again, eluent air-dried after the nitrogen with the nitrogen methyl imidazole and the trifluoroacetic acid bitter wine grows, the derivative with the highly effective liquid chromatography analysis, used the external standard legal quantity.

通过田间试验建立了阿维菌素在甘蓝及土壤中的残留分析方法，并对其在甘蓝和土壤中的残留进行了测定，样品用乙腈提取，提取液旋转浓缩近干后用少量乙腈溶解，再经PRS固相萃取柱净化，洗脱液经氮气吹干后用氮甲基咪唑和三氟乙酸酐衍生，衍生物用高效液相色谱分析，采用外标法定量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。