定量测定

A gas chromatographic method to determine the conversion of asymmetric hydrosilylation of acetophenone has been established.

建立了由气相色谱法测定苯乙酮不对称硅氢化反应转化率的定量方法，操作简便、迅速，重复性和精密度较好，多个样品各重复6次的变异系数均小于1％，且结果与1HNMR法测定结果一致。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

The ci group, control groups and part of ci group with the same level of blood pressure were subgroup by the result of questionnaire, correlation analysis was made between the amounts of blood markers and ill performance characteristics of tabp. results 40% ci patients were tabp.

对100例ci患者和107例无血液病和肿瘤的对照者进行a型行为评定量表测定，并同时对两组人员的血管内皮细胞损伤的血浆标志物内皮素和血栓调节蛋白含量进行测定；再根据a型行为问卷调查结果，分别对ci组和对照组患者，以及在同一血压水平的ci患者按照个体的行为特征进行分组，然后对其血浆标志物浓度进行相关分析。

This study is based on the Stanford equation and aims at getting high yield and high quality rice. With the aid of stage and monomial method of quantitative determination, the three parameters of the precise and quantitative N application theory and technology were measured and validated.

以水稻高产优质为目标，以斯坦福方程为理论基础，配合现有阶段性的、单项的定量测试方法，对水稻精确定量施氮技术理论的3个参数进行测定及验证，初步找到了一些研究思路和方法。

The experimental conditions were optimized to produce following results: good linearity of sulfamethoxazole, norfloxacin, ciprofloxacin, ofloxacin and tetracycline in the range from 1.00 ng/L to 1 000 ng/L and the limits of quantification 1 ng/L, the good linearity of terramycin and aureomycin in the range from 10.0 ng/L to 2 000 ng/L and the LOQ 10 ng/L, the recoveries of deionized water and surface water from 72.1% to 112%, and the RSD from 0.6% to 9.2%.

摘要〗采用超高效液相色谱-电喷雾二级质谱联用技术同时测定水中7种抗生素，优化了试验条件。磺胺甲恶唑、诺氟沙星、环丙沙星、氧氟沙星、四环素在1.00 ng/L ～1 000 ng/L 范围内线性良好，定量下限为1 ng/L ，土霉素、金霉素在10.0 ng/L ～2 000 ng/L 范围内线性良好，定量下限为10 ng/L ，去离子水和地表水样加标回收率为72.1%～112%， RSD 为0.6%～9.2%。

In this paper,the response factors of thermal conductivity detector of monochloromethane and dimethyl ether are estimated and calculated by using group cross section area method,molecular diameter method and homologue linear rule method.

热导校正因子的估算以及在工业氯甲烷生产中的应用李洪宽（山东省化工研究院色谱中心济南250014）关键词气相色谱法，一氯甲烷，热导检测器，校正因子1前言热导检测器定量校正因子的测定和计算是气相色谱热导定量的关键。

The total nitrogen was determined by ultraviolet spectrophotometer, and the absorbency needed to be emendated by A=A(subscript 220nm)-2A(subscript 275nm) The total phosphorus with sulfuric acid-ammonium stibium molybdate-ascorbic acid as the developer was determined by spectrophotometer at 700-720nm wave length.

用紫外分光光度法测定总氮，并采用校正吸光度A=A(下标 220nm)-2A(下标 275nm)对其进行定量；用钼－锑－抗作显色剂，在波长700~720nm下对总磷进行比色定量。

Along with the development of the natural science people deepen the color knowledge gradually, for example, written language description is not contented to the needs in some field, and the need is quantitative analysis. Color index can show the characteristics of color exactly. For adapting the development, the authors use the metal-bearing ore's color index analysis for the first time through reflectance determining and the color-imetry theory, and analyses 4 turquoises' color index in various color. So the color of turquoise is quantitated successfully.

随着自然科学的发展，对颜色的认识不断加深，从定性到定量颜色指数可以准确地描述颜色特征，笔者利用色度学原理，通过反射率测定，首次把金属矿物颜色指数分析方法应用于绿松石，分析了4个不同颜色绿松石的颜色指数，成功地实现了绿松石颜色的定量表示。

In order to establish a quantitative method in detecting HIV 1 proviral burden and viral load, 8E5 cell line and a recombinant RNA constructs were used as the HIV 1 proviral DNA and viral RNA external references, respectively.

以定量的HIV 1DNA和RNA为标准外参照，利用SYBRGreen荧光染料和GeneAmp5 70 0SequenceDetectionSystem (5 70 0系统)，建立了测定HIV 1的前病毒载量和病毒载量的荧光实时定量PCR技术。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。