定量测定

These findings suggest that FCM is a rapid and useful technigue in the analysis of pleural effusion and can be a very useful adjunct to conventional cytopathology.

基於此研究结果，在胸水诊断价值上FCM DNA定量方法是一种快速，有用的分析定量胸水细胞DNA，尤其是和一般临床细胞诊断方法同时测定更为有效。

Or cytologic examination, the samples were prepared using standard techniques. Sample for FCM analysis were centrifuged and exposed to hypotonic solution cotaining detergent and propidium iodide. Thirty-eight patients had pleural effusion due to benign disease, whilst 33 patients had primary lung cancer. All 38 patients with benign pleural effusions showed FCM diploidy.

CM之DNA定量法是测定细胞是否为不正常DNA含量，其染色是用propidium iodide染色，临床细胞诊断法是用Dapanicolaou染色方法，其中38位病例之胸水原因是良性病变，33位胸水病例则有原发性肺癌，38位良性胸水病例之FCM DNA定量结果全部为正常含量。

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛，包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等，已广泛应用于医学临床，它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

Pd could be quantificationally separated from Sb, Zrand Ce by flotation when the concentrations of potassium bromide and tetrabutyl ammonium bromide in solution were 1.5×10^(-2) molL^(-1) and 2.0×10^(-3) molL^(-1), respectively. The method was used for the separation and determination of Pdin the sample of synthetic water with satisfactory results.

当溶液中嗅化钾和四丁基溴化铵的浓度分别为1.5×10^(-2)molL^(-1)和2.0×10^(-3)molL^(-1)时，钯被定量浮选，实现了钯与锑、锆、和铈离子的定量分离，对合成水样中的钯进行定量浮选分离测定，结果满意。

Take DMSO swollen treatment and DEA-SO2-DMSO decrystallization treatment for example, the X-ray diffraction and the Tobolsky's intermittent stress relaxation of treated woods were determined during soaking in water, analyzed the effects of water on crystal degree and inter-cohesion of treated woods. According to these continuous relaxation curves measured in water with different temperatures, various thermodynamic quantities were obtained by using Eyring absolute rate theory, and reviewed the chemical reactions in wood which occur in different relaxation process. For the first time quantify these crosslinkings formed in the process of tensional relaxation by using the SMCIR intermittent stress relaxation way, and defined the cross-linking reaction types. In order to find out the contribute of drying to the fixation of deformation of chemically treated wood, stress relaxation of oven-dry untreated and treated wood was measured during the process of temperature elevation and descend, then analyzed the effect of temperature change on relaxation mechanism of treated oven-dry wood. According to continuous relaxation curves of oven-dry treated wood under various constant temperature, calculated the thermodynamics of relaxation process and discussed the mechanism of molecule change in wood, at the same time, also quantified these cross-linkings produced in wood by intermittent method and on the basis of which the model of molecular change during relaxation process of chemically treated was constructed.

以DMSO膨胀处理及DEA- SO2-DMSO非晶化塑化处理为例，测定了两种处理木材在水浸渍过程中的X射线衍射及Tobolsky间歇应力松弛，分析了水对处理木材结晶度及内部凝聚力的影响；通过未处理和两种处理木材在不同温度水中的连续应力松弛测定，应用Eyring的绝对速度反应理论计算并获得了松弛过程中的各热力学量，分析了在水中松弛过程中不同阶段木材内部发生的化学反应；并首次采用SMCIR连续·不连续双曲线应力松弛法定量了轴向拉伸应力松弛过程中木材内部产生的架桥量，明确了交联反应的类型；为了了解干燥对处理木材塑性变形固定的影响，测定了未处理和两种处理绝干木材在温度下降过程和上升过程中的应力松弛，分析了温度变化对处理绝干木材应力松弛的影响；根据多个温度水平下的连续应力松弛测定曲线，计算松弛过程的热力学量，考察了绝干木材在松弛过程中内部发生的分子变化机理，同时也用间歇法定量了木材内部新形成的架桥量，并在此基础上构筑处理木材在松弛过程中内部分子构造的变化模型。

Analysis on main points for determination of chlorine dioxide by iodometric method ;2. The experiment results show: To measure sulfide in wastewater by iodometric method may have errors because the interferent can not be removed.

用碘量法测定废水中的硫化物，可能因无法消除干扰而使测定结果存在较大误差；对于同一废水样品，当碘量法和亚甲基蓝分光光度法的测定结果存在显著性差异时，可用Pb2半定量法进行判定；对于某些化纤企业的废水，用氢氧化钾-乙醇溶液洗涤沉淀，可消除碘量法测定的干扰。

Image analysis system was used for semi-quantitive assay of TGF-βRⅠ and R Ⅱexpression, and microphotography was used to record the morphologic changes of culturedchondrocytes. The results are:1. TGF-〓 showed sequentially inhibitory and promotive effects on the proliferation of human articular chondrocytes of two week monolayer culture from one passage to eight passage.

本研究采用CellTiter 96〓单溶液细胞增殖分析试剂盒测定体外培养中的软骨细胞的增殖活细胞数量；免疫组化法测定软骨细胞TGF-βR Ⅰ、R Ⅱ，Ⅱ型胶原和S-100蛋白的定性表达；酶免分析法测定TGF-〓作用后的Ⅱ型胶原定量表达；通过图象分析仪，对TGF-βRⅠ、R Ⅱ的免疫组化染色结果进行半定量分析；本研究的实验结果如下：1。

The activity of erythrocyte AR and 〓-ATPase, the level of the plasma NO and NOS, the content of sorbitol in sciatic and erythrocyte were detected by spectrophotography; The level of ET in plasma were detected by radioimmunassay .

荧光法测定红细胞AR活性；分光光度法测定红细胞膜〓-ATPase活性、血浆NO及NOS水平、坐骨神经及红细胞内山梨醇含量：放射免疫方法测定血浆ET水平：RT-PCR方法半定量坐骨神经内ARmRNA水平。

In severe patients, the change of CRP and cTnⅠ was higher than the mild patients.

以化学免疫荧光法定量测定血清CRP及cTnⅠ水平。

Apply Barro to return to equation to combine gravitational model to arrived 1995 to area of economy of sea of the Huaihe River the side effect between 2005 undertook ration determines, reach Sulu pleaseds Anhui 4 provinces side effect is existing unifinication border and alienation border 2 sort, unifinication border includes Su Lu border, Su Wan border, Yu Wan alienation of attrib border; border includes Lu Yu border, Lu Wan attrib border He Suyu is attrib border.

运用Barro回归方程结合重力模型对淮海经济区1995年到2005年间的边界效应进行了定量测定，得出苏鲁豫皖4省边界效应存在着一体化边界和疏远型边界2种类型，一体化边界包括苏鲁边界、苏皖边界、豫皖边界；疏远型边界包括鲁豫边界、鲁皖边界和苏豫边界。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。