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All the synovia specimens were harvested for the RT-PCR method to detect the mRNA expression of MMP-1、MMP-3、IL-1β and TIMP-1, to see the effect of estrogen on the OA cartilage and the possible pathogenesis of estrogen.

对兔膝关节OA滑膜组织中MMP-1、MMP-3、IL-1β和TIMP-1 mRNA水平进行RT-PCR半定量测定,了解雌、孕激素对OA软骨中上述指标的表达情况,以了解雌激素对OA的确切作用,探讨其可能的作用机制。

The extracts of Torreya grandis epispem were obtained by both supercritical CO2 extraction and steam distillation, and their chemical compositions were analyzed by GC-MS qualitatively and quantitatively.

分别采用超临界CO2萃取法和水蒸气蒸馏法提取香榧外种皮提取物,并采用GC-MS分析手段对提取物的化学组成进行了定性和定量测定

CD was added into running buffer in above electrophoresis system, and the separation efficiency of polycarboxylic acids was improved further by the supramolecular interaction. Based on that, the method for separation and determination of several di- and tricarboxylic acids, including oxalic, malic, tartaric and citric acids, found in fruit juices was developed, which supplied a fast, convenient and sensitive method for the food analysis, the quality control of foods and various drinks.

果汁中多元有机酸的毛细管区带电泳/Cu电极安培检测在上述分析体系的电泳介质中添加β-CD,利用CPB与β-CD的协同超分子作用,进一步提高了多元有机酸的分离效率,成功实现了水果中四种常见多元有机酸草酸、柠檬酸、苹果酸、酒石酸的基线分离,建立了几种果汁中上述有机酸毛细管区带电泳/Cu电极安培检测定量测定方法,为食品分析、食品饮料质量控制以至辨别酒类商品真伪等提供了一种方便、快速、灵敏度高的分析方法。

Objective :To quantify bacterial endotoxin in the raw materials of tobramycin for injection by dynamic turbidimetric Limuius test.

目的:应用动态浊度法鲎试验定量测定妥布霉素原料药中细菌内毒素。

Objective:The kinetic turbidimetric Limulus test was used in the endotoxin assay of Ligustrazin Hydrochloride injection.

摘 要 目的:应用动态比浊法鲎试验定量测定盐酸川芎嗪注射液中的细菌内毒素含量。

A method is introduced for quantitative determination of 1,2-dichloride ethylane by gas chromatography with PEG-20M chromatographic column,FID and using undecane as internal standard.

介绍用气相色谱仪、以PEG-20M色谱柱和FID检测器对1,2-二氯乙烷进行的一种定量测定方法,其内标物选用正十一烷。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点,用免疫组织化学方法(抗NF-κB p65抗体、ABC法,石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率,采用单变量多因素方差分析和Q检验做统计学处理。

The DNA relative content of large unreduced pollen grains induced by colchicine was determined quantitatively with laser scanning confocal microscopy using the normal-sized pollen grains as control. The results suggest that the large unreduced pollen grains are diploid.

以正常花粉作对照,用激光扫描共聚焦显微镜对经秋水仙碱处理诱导出的大花粉进行了DNA含量的定量测定,初步确认该试验诱导的大花粉是一种二倍体花粉。

Methods The expressions of TNF-α and TGF-β1 proteins in uteroplacental tissues were detected with immunohistochemistry in 28 early spontaneous aborters and 28 normal early pregnant women.

采用免疫组织化学技术半定量测定28例早期自然流产患者和28例正常妊娠妇女绒毛和蜕膜组织内TNF-α及TGF-β1表达强度。

All the cases were phlebotomized venously 8ml under fasting condition in the morning.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测,血清IL-6 采用放免法检测,血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定

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