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定量测定

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The useful and important tests of sensibility are: testing the vibratory sensation with tuning fork, vibrometer testing for vibratory thresholds, von Frey testing (determined by Semmes- Weinstein nylon monofilaments or Pressure- Specifying Discriminator) for cutaneous pressure thresholds, measurement of static and moving two- point discrimination with the Disk- Criminator, Moberg's ninhydrin test, O'Rain skin wrinkling test, Picking- up test, Tinel's sign and electrodiagnostic test.

此文对临床上重要且实用的皮肤感觉功能测定方法如音叉皮肤振动觉定性检查、振动觉阈的振动器定量测定、Semmes-Weinstein尼龙单丝或Pressure-SpecifyingDiscri minator行皮肤压觉阈的测定、用Disk-Criminator进行静止和运动的两点辨别觉测定、茚三酮试验、O'Rain指腹皮肤皱缩试验、捡物试验、Tinel's征电生理检查以及这些检查方法在神经断裂、神经卡压及神经损伤或修复后的恢复等情况中的应用和评价作一讨论。

According to the predacity of the nematode-trapping fungi and the growth habit of pine wood nematode Bursaphelenchus xylophilus, this paper tested the B. xylophilus that cultured by the fixed time and quantities. Trying to find out the quantificational test method of trapping rate, by the change of the quantities of B.

本文依据捕食线虫真菌的捕食特性以及松材线虫的生长习性,对松材线虫进行了定时、定量的测定试验,试图通过培养后线虫的种群数量变化,来摸索定量测定捕食率的方法。

For NIRS quantitative analysis, three quantitative models were built. They were three quantitative models of norflocaxin capsules from different manufacturers, the bi-component quantitative models of pulvis fellis ursi and the quantitative model of scutellaria.

对于近红外定量分析,建立了三个定量模型,分别是:三种多厂家诺氟沙星胶囊诺氟沙星胶囊定量测定模型、熊胆双组分定量测定模型和黄芩中黄芩苷定量测定模型,并对各模型的专属性和准确性进行了评价。

With the observation of the outward appearances, it can be known roughly that if the sample was added with adulterations. Since the beeswax dissolves in the benzene but the adulterations, such as gypsum, starch and so on do not, these can be determined. By the verification of acid value and alcohol dissolve ability, the acid or non acid added can be determined. By the change of saponification value, the ester or non ester added can be determined. By the carbonification degree in the concentrated sulphuric acid, the mineral wax, such as paraffin wax added can be determined.

通过样品外观的观察初步定性是否掺有杂质;利用纯蜂蜡在苯中溶解,而掺入的石膏、面粉和淀粉等杂质在苯中不溶而定量鉴定这类杂质;由酸值变化和乙醇溶解物的量,测定掺入的硬脂酸等酸性物质或其它的非酸性物质;由皂化值变化,定量测定掺入的动物脂等酯类物质或其它的非酯类物质;根据蜂蜡中的烃类在浓硫酸中可被炭化,加入的石蜡等矿物蜡不被炭化来测定此类掺杂物。

Cases healthy subjects served as control. In addition, density of glomerular matrix membrane and quantity of cell in glomeruli were measured with CMIAS image analysis system by computer. Global sclerosis, cellular crescents, fiber or fibrocellular crescents were measured with Memphis scope. Tubulointerstitial lesions were examined with semi-quantitative grades (including O-III grades).Results Compared to normal control, there was a significant increase of urinary TGF-betaK IL-6 and Col-lV levels in patients with IgAN (p. 01). There were also significantly positive correlation between the levels of urinary TGF-betaK Col-IV and density of glomerular matrix membrane ., interstitial fibrosisp.

采用酶联免疫吸附法测定尿TGF-β1(Transforming growth factor-β1)、尿IL-6(interleukin-6)和尿Col-Ⅳ(type Ⅳ Collagen);采用免疫组织化学法检测肾组织TGF-β1、Col-Ⅳ表达;应用CMIAS多功能真彩色病理图像分析系统对肾小球基质基底膜面密度和细胞个数进行半定量测定;对球性硬化、节段硬化、细胞新月体及纤维或纤维细胞新月体所占肾小球百分数和肾小球IgA沉积免疫荧光强弱的判断采用Memphis法进行评分;肾小管间质病变程度采用光镜下半定量分级法(包括0-Ⅲ级)。

Based on the analysis and comparison of various methods of mensuration of effective ingredients of indigowoad root,phenol-vitriolic colorimetry has been choosen to be the method of mensuration of amylose in indigowoad root, and standard amylose graph has been made,in which c=22.476A+0.5309, correlation rate r=0.9970; Direct colorimetry has been choosen to be the method of mensuration of indigotin and Indirubin,and based on all above,the maximal absorb wavelenghes of indigotin and Indirubin have been determined through experiment.

在分析比较各种板蓝根有效成分测定方法的基础上,选用苯酚-硫酸比色法作为板蓝根多糖的定量测定方法,作山了多糖的标准曲线:c=22.476A+0.5309,其相关系数r=0.9970;选择直接比色测定法作为靛蓝、靛玉红的测定方法,并在此基础上通过试验确定了靛蓝、靛玉红的最大吸收波长及显色稳定性。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成:一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下:参照 GB/T16886.5-1997-ISO 10993-5:1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求,采用规定的 L929 细胞(小鼠结缔组织成纤维细胞),分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试,采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化,并在显微镜下照相,从而对细胞与材料的生物相容性进行定性评价;同时采用细胞生长抑制法,以酶标仪定量测定评价各组 1,2,4,7 天 L929 细胞的相对增殖率,以定量测定并判别材料对细胞的毒性程度。

In this paper,the Graphite Furnace Atomic Absorption Spectrometry was applied to the determination of molybdenum in lixiviated solution discharged from plants of producing Fe and Mo.

用石墨炉原子吸收光谱法测定钼铁厂固体废物浸出液中的钼,当选择合适实验条件的情况下,钼的测定灵敏度较高,校准曲线的相关系数大于0.999,精密度较好,方法的检出下限为0.6μg/L,定量测定范围2.4~60μg/L,测定结果令人满意

We collected venous blood of 4 millilitre from all the children,and quantitative determination blood LPS, LBP, mCD14 by limulus reagent kinetic turbimetric assay, flow cytometry technique and ELISA method.

所有SIRS患儿及正常儿童各取静脉血4ml,分别利用鲎试剂动态比浊定量测定法、流式细胞分析技术、酶联免疫试验进行血液内毒素、mCD14、LBP的定量测定

Several methods were selected to determine the content of flavonoids in corn silk, while satisfying wave length wasdecided by means of UV-visible photometer curves of flavonoids and its monomer in corn silk, as well as rutin at the range of200~600nm after reacting with different regents , and comparison of experimental results were carried out.

本文选用多种黄酮类测定方法,通过对玉米须黄酮类提取液、单体及芦丁标样用不同的显色剂反应后,测定各反应液在200~600nm范围内的吸收曲线,确定了适合玉米须黄酮类定量测定的波长,并对不同测定方法的定量结果进行了比较。

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