定量分析

Methods Thirty eight patients with loosening of hip prosthesis were divided into three group: total hip arthoplasty, double hip arthuplasty and integrated arthoplasty. The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph, and the content of TNF in samples of interface membranes was determined.

方法］根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分为3组：全髋置换组、双动头组和一体式单动头组，对各组术中松动假体周围界膜组织进行光镜下炎性细胞计数的半定量分析和肿瘤坏死因子的测定，分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

［Objective］To study the relativity during polyethelene debris cytokine and loosening of hip prosthesis.［Methods］Thirty eight patients with loosening of hip prosthesis were divided into three group:total hip arthoplasty,double hip arthoplasty and integrated arthoplasty.The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph,and the content of TNF in samples of interface membranes was determined.

［目的］研究聚乙烯微粒、细胞因子与人工髋关节松动的相关性［方法］根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分 3组：全髋置换组、双动头组和体式单动头组，对各组术中松动假体周围界膜组织进行光镜下炎性细胞计的半定量分析和肿瘤坏死因子的测定，分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

METHODS: Thirty six SD female rats were randomly separated into 3 groups (n=12 in each one) according to the infarction duration (1, 3, 28 d), which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d after AMI were detected by RT PCR and analyzed semi quantitatively by image analysis system.

将36只SD雌性大鼠按时间段随机分为3组（每组12只），每组再随机分为假手术组、手术组2个亚组（每组6只），应用RT PCR方法检测大鼠AMI后1，3，28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达，并以图像分析系统对其进行半定量分析。

Tissues of the infundibular septum from 22 patients with TOF were studied and 8 patients without cardiovascular disease and collagen system disease were used as control.The collagen concentration were determined by biochemical methods,the heart function were determined by pressure curve in right ventricle.

用生化方法定量分析22例法洛四联症患者和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ/Ⅲ型胶原比值，并用右室穿刺法测定反映法洛四联症患者右室收缩及舒张功能的dp/dtmax及-dp/dtmax。

The objectives of the study were to investigate the characteristics and influencing factors of myocardial interstitial collagen of right ventricle in tetralogy of Fallot.Tissues of the infundibular septum from 22 patients with TOF were studied and 8 patients without cardiovascular disease and collagen system disease were used as control.Collagen were determined by biochemical methods.

为了解法洛四联症肥大右室心肌间质Ⅰ、Ⅲ型胶原改建的特点及其影响因素，用生化等方法定量分析了22例法洛四联症病人和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ、Ⅲ型胶原比值，以及法洛四联症病人的血氧饱和度等影响因素。

Methods Samples of the infundibular septum from 22 patients with TOF and 8 patients without cardiovascular disease and collagen system disease as control were studied. Collagen was determined by biochemical methods, Pro α mRNA, Pro α mRNA levels were determined by dot blot hybridization.

用生化方法定量分析了22例法乐四联症患者和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ、Ⅲ型胶原比值，以及用斑点杂交法测定法乐四联症患者右室心肌间质Ⅰ、Ⅲ型前胶原基因mRNA的表达。

The quantitative determination of inosine and inosinic acid was conducted by High Performance Liquid Chromatography.

采用高效液相色谱对风味酱中的肌苷、肌苷酸进行定量分析。

The quantitative determination of inosinic acid and inosine was conducted by high performance liquid chromatography.

采用高效液相色谱法对藏系绵羊肉中的肌苷、肌苷酸进行定量分析。

Observation with transmission electron microscope showed that interalveolar septa were widened with increased amount of collagen in the MP infected rats while there were no obvious abnormalities in the other two groups.(2) Positive expression of bFGF mRNA were found in alveolar walls of MP infected rats. No expression of bFGF mRNA was found in control animals. In the rats infected with MP but treated with erythromycin positive expression of bFGF mRNA was found to be scarcely distributed in alveolar walls.

2感染组动物肺泡壁见明显的bFGF mRNA阳性表达颗粒，对照组基本未见bFGF mRNA阳性表达，感染加红霉素治疗组仅见散在分布的少许bFGF mRNA阳性表达；定量分析结果表明，感染组bFGF mRNA阳性表达的积分吸光度为41.32±10.44，与对照组(0.30±0.13)和感染加红霉素治疗组(6.03±2.41)比较，差异有显著性( P ＜0.01)。

Methods Five groups (n=24) of SD rats were randomly assigned to received intrapleural injection of dexamethasone, dipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR.

SD大鼠按实验设计分为5组(n=24)：地塞米松组、内毒素组、红霉素组、高渗盐水组及对照组，通过免疫组织化学染色测定AQP1在胸膜上的表达和定位；采用Real-time RT-PCR方法对不同刺激因子作用下不同时间胸膜组织AQP1的基因表达进行定量分析。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。