定向进行

The whole brain of the subject was axially and coronally scanned in the normal stereotactic space with a slice 1mm thick by MR system. The caudate nucleus were identified, segmented, extracted, saved and three-dimensionally reconstructed on MRI by software system developed by Research Center of Biology, Harbin University of Technology.

在以AC-PC线为基准线的标准脑立体定向空间对志愿者做1mm层厚，间距0mm的MR轴位和冠状的脑扫描，利用哈尔滨工业大学生物研究中心研究开发的软件系统对经MRI扫描后转存的尾状核图像进行三维重建，软件提取分割的尾状核轮廓后执行重建命令。

Objective To study the method of inducing human marrow mesenchymal stem cells into osteoblasts directionally and to identify osteogenesis characteristics.

目的：探讨将成人骨髓间充质干细胞定向诱导分化为成骨细胞的方法，并对所诱导细胞的成骨特性进行鉴定。

Some problems that develop with the geological survey of directionally drilled river are summarized and analysed.

对管道定向钻穿越河流的工程地质勘察工作中出现的一些问题进行了总结和探讨，认为勘察钻孔工作量的布置应随场地条件而异，科学合理地安排，而不宜教条地执行规范、规程中的某些条款。

The linear plasmid and the designed fragment were directively ligated with T4 DNA ligase,and the plant expression vector of carrot afp was constructed.

将目的片段与线性质粒在T4 DNA连接酶的作用下进行定向连接，构建成胡萝卜afp的植物表达载体pBAF。

This longitudinal study is intended to examine relationships among dispositional goal orientation,social self-efficacy,and cultural adjustment.Data were collected from 176 freshmen in a university in China.

通过对176名大学新生3个月的纵向研究，采用探索性因素分析、信度分析和回归分析等方法对目标定向与适应的关系以及社会自我效能感在其中的中介作用进行了深入研究。

The different factors involved in the process of the folding and activation of the microbial lipase are reviewed in this article,including lipase-specific foldase,lipase activation factor,prosequence,calcium ion and disulphide bond.

对微生物脂肪酶活性构像的形成和激活机制进行深入研究，有助于我们更好地了解脂肪酶的结构和功能，为脂肪酶基因的大量表达和体外定向进化奠定基础。本文主要综述了作为脂肪酶结构成分的多种因子在脂肪酶活性构像形成、激活及稳定中的作用。具体催化反应体

It was found that a small amount of α-Fe dendrites with equiaxed morphology existed in specimens grown at 200 and 500 μm/s.

中文摘要：对Nd14Fe79B7合金进行了定向凝固试验与形核有关的凝固组织形成研究。

Secondly, kinematics of the frameless stereotactic surgical robot is analyzed with D-H notation.

其次对课题开发的无框架立体定向手术机器人进行了运动学分析。

In this dissertation, we purify,functionalize, and align the single-walled carbon nanotubes by Langmuir-Blodgett technique using compression-transfer method.

本文正是针对这一问题，对单壁碳纳米管进行了提纯、功能化，并利用LB技术的液面排布转移法实现了碳纳米管沿着轴向大面积定向排列。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。