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A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物,自接种H5N1亚型病毒的鸡胚组织中提取RNA,反转录后采用高可信度DNA聚合酶经PCR扩增M1基因,采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达,纯化获得N末端携带多聚组氨酸标签的重组蛋白,分子量约29.8 kDa。

According to Andy Mazzara, the director of the Institute for Non-Lethal Defence Technologies at Pennsylvania State University, prototypes of such devices use a laser to create an ionise d channel of air through which an electromagnetic pulse is directed.

据宾夕法尼亚州立大学的非致命防御技术学院的主任 Andy Mazzara 说,这些设备的样本模型利用激光创造空气离子波道,电磁脉冲通过波道定向。

HMW-GS 5+10 was transferred to 14 wheat cultivars of Heilongjiang province through 5-6 consecutive backcrosses with biochemical marker assisted selection. HMW-GS 2+12 and 5+10 NILs of 9 wheat cultivars have were analysed.

利用连续的选择性回交(回交5到6代)结合&半粒法&和生化标记辅助选择,在国内首次将对烘烤品质有重要贡献的编码5+10等优质亚基的基因定向转移到黑龙江省14个小麦主栽品种中。

The following is a cached copy of the requested page, and may not be up to date.

讨论 以下的重定向页指向的是不存在的条目。

Talk The following is a cached copy of the requested page, and may not be up to date.

Talk 以下的重定向页指向的是不存在的条目。

This indicates that Grade 2 was not sensitive to cue validity,so they did not make use of predictability of cues to voluntarily adjust exogenous orienting triggered by cuing.

这表明,2年级被试对高线索、低线索并不敏感,并没有运用线索的有效预测信息自主的调整由线索化引发的外源性注意定向。

This paper claims that the latest development of international private law shows the following trends:the scope is broadening and new theories constantly emerge; legal connections separate and connective factors are elasticized; more attention is paid to substantive law and the application of policy direction and result selection;restrictions of lex voluntatis are strengthened, contractfulfilling place becomes specific and concrete; lex domicile replaces lex patriate, and lex fori has the priority; attitud...

本文认为,国际私法当今发展的动向是:1。内容范围不断扩大,新学说不断涌现;2。法律关系分割化,连结因素弹性化;3。重视实体法解决的方法,重视政策定向、结果选择的运用;4。&意思自治&限制加强,契约履行地特定具体;5。住所地法代替本国法,法院地法优先适用;6。反致态度发生改变,第三世界登上国际私法舞台;7。国内立法法典化,国际立法多样化

For better manifestation of colours in anisotropic transparent stones the orientation of faceting along optical axes is necessary.

要使非均质性透明宝石矿物的颜色更好地表现出来,沿光轴方向的加工定向是非常必要的。

The enzyme activity was determined according to the velocity of the formation of halos on the plates.

建立了双层平板筛选由定向进化方法产生的瑞氏木霉内切葡聚糖酶Ⅲ突变体文库的方法,根据水解圈在平板上形成的速度判断酶活高低。

In the way of prophase preparing, this paper emphasizes the importance of full collecting inhering under pipe using no-dig horizontal directional drilling paving in the busy street and emphasizes the exploration technology being the main mean of inhering under pipe. Thus it can provide the main pledge measure of under pipe security and operator security.

在前期准备方面,本文强调在闹市区采用非开挖定向钻进铺设地下管线时全面收集原有地下管线资料的重要性,强调物探技术是对原有地下管线探查的重要手段,即保证原有地下管线安全性及操作人员人身安全的重要保证措施。

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推荐网络例句

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特,那个是爱朗。

Articulate the aims, objectives and key aspects of a strategic business plan.

明确的宗旨,目标和重点战略业务计划。