完整

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

The similarity logic calculus systems based on incomplete information and incomplete case knowledge is constructed by using a first order modal logic and a simplified second order predicate calculus. The grammar、semantics and inference rule are described.

采用传统的一阶模态谓词逻辑和简化的二阶谓词逻辑分别构建了基于信息不完整和实例知识不完整的相似性逻辑演算系统IISL和IKSL，给出了系统的语法、语义和推理规则。

Based on it, detailed computational procedure package and curve storehouse of arbitrary kinds of Mathieu function has also been obtained.

并在此基础上给出了完整的计算程序包，建立了目前最为完整的Mathieu函数曲线库。

The embodiment of the invention discloses a call connection method used for an IP IMS. The method comprises the following steps: the portal element of a called network receives a session request of a calling network; user identification information is contained in the session request; when the portal element of the called network judges that the identification information of a called user is incomplete, the portal element of the called network sends the calling network a responding message which indicates that the identification information of the called user is incomplete; the calling network updates the identification information of the called user according to the responding information and transmits the updated identification information of the called user to the portal element of the called network.

本发明实施例公开了一种IP多媒体子系统中的呼叫接续方法，包括：被叫网络入口网元接收主叫网络的会话请求，在会话请求中包含被叫用户标识信息；当被叫网络入口网元判定所述被叫用户标识信息不完整时，向主叫网络发送指示所述被叫用户标识信息不完整的响应消息；主叫网络根据响应消息更新被叫用户标识信息，并向被叫网络入口网元发送所述更新后的被叫用户标识信息。

Verse metrically complete; especially having the full number of syllables in the final metrical foot.

韵律上完整的；特别是在最后一个韵脚有完整的音节数。

Having a metrically complete pattern , especially having the full number of syllables in the final foot .

押韵的，完全韵脚的：格律完整的形式，尤指最后的韵脚其音节数完整。

Having a metrically complete pattern,especially having the full number of syllable s in the final foot.

押韵的，完全韵脚的格律完整的形式，尤指最后的韵脚其音节数完整。

The innovation of this paper is in order to make a systemic and integrated research of 'hinese milch cow industry, based on the previous research.

本文的主要创新是在综合国内外对于奶牛业研究成果的基础上，力图比较全面、系统地对中国奶牛业粗放式发展过程中各个环节存在的问题进行较为详细的探讨、分析与论证，力图使国内对中国奶牛业的研究达到一个由割裂到完整的状态，为建立一个系统、完整的中国奶牛业研究体系而努力。

If you have a new sweetie , it 's easier to misstep on this special day .

柏拉图认为对爱的追求，重点不在于是追求"完整的爱"的欲望，而在于这个完整的全体必须是好的，必须是善的。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。