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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

Influence of dry or wet bonding on microleakage of Ti alloy inlay;2. Influence of dry or wet dentin bonding surface on wet bonding strength: a microtensile study;3. To study the microstructural influence of dry or wet dentin bondin g surface on the dentin bonding interface, five wet bonding systems were used to bond Chrisma B20 resin with dry or moist dentin surface with scanning electron m icroscope.

结果湿粘结时5种粘结系统在粘结界面获得良好的渗透图象;干燥粘结时,两种以丙酮为溶剂的粘结系统在干燥粘结时形成的粘结界面,与湿粘结时有明显的区别,混合层较薄,并有明显的不完全渗透的结构和混杂层的形成;以酒精和水为溶剂的粘结系统在两种不同粘结状态时粘结界面的超微结构没有明显的区别。

But when we mine commensuration groundwater, the falling velocity water level and range of the deep hydrous stratum are bigger than the shallow hydrous stratum. And the quantity of subsidence is not obvious different. 2. Analyzing the relation between the stratum of groundwater and land subsidence, with the depth of aquifer bed increased, the proportion that the released water because of compressibility of strata possessed become bigger, and it cause land subsidence more serious. 3. The physical and mechanical properties and the consolidation state of stratum do not become better with the depth increased.

依据: ①一般情况下,随深度增加,地层的压缩性会渐下;但是开采同量的地下水,其水位下降的速率及幅度深部含水组比浅部含水组要大的多,两者引起的沉降量不会有明显的差别;②从地下水开采资源组成与地面沉降关系分析,含水层深度越深,其中的压密释水量所占的比例也越大,造成的地面沉降也越严重;③地层的物理力学性质及固结状态,随深度的增加,也不完全是越来越好。

Total submergence largely decreased biomass production and growth in length,with less effect on shoot numbers.Increased elongation of the growing internodes of up to 140% is caused by submergence.Nutrient such as N,P,K do significantly affect on growth.No mater lack of any element will decrease the biomass,the result same as the oligotrophic conditions.But lack of phosphorus is the worst mater.

而淹没式淹水可能使生物量降低,但是植株的高度有所增加,因为淹水可以使芦苇的节间高度提高140%;对根茎的数量没有很明显的影响。N、P、K等营养元素对芦苇生长也具有重要的作用,无论缺少哪一种对芦苇生长都不利,跟完全贫营养状态没有明显的区别,但是缺P时芦苇生长是最差的。

The decreased threshold shear velocity and unsaturated sand flows at the stoss slope of the dunes resulted the erosion at the toe of the dune. Under the medium and low drifting-sand winds the sands being transported have divergence with that on the surface, the mean grain size of transported sands is lower than that on the surface and the sand transporting is a stochastic process.

起动剪切速度减小与不饱和风沙流是沙物质在横向沙丘迎风坡坡脚不能产生沉积的主要原因;再者,平均风速并不能完全反映野外实际状态下气流的性质,在低-中强度的风沙活动过程中,被输送的沙物质的粒度组成与下垫面物质有明显的差异。

The research indicated that, the structure inside the pure C8TES SAM was indeed loose and unassembled completely, the molecule density inside the SAM was very low, which would result in an ultra-low surface contact angle, only 20~21°, on the C8TES SAM surface.

我们的研究表明,由于C8TES分子头部三个乙氧基形成的空间(来源:b5e5ABe3C论文网www.abclunwen.com)位阻的影响,C8TESSAM的内部处于一种不完全组装的松散状态,所形成的自组装单分子膜内分子密度偏低,造成C8TES SAM的表面接触角偏低,只有20~21°。

A series of gyroscopes in Murata Girl's body help her to stay balanced, even when stationary.

通过内置的多个感应器配合智能调节装置保持机器人近乎完美的平衡状态,甚至在完全静止的时候都可以纹丝不动。前置的超声感应装置还可以让机器人在遇到无法逾越的障碍物时自动停止,避免事故的发生。

The thesis mainly concerns the following aspects: the relay protection and its trip-close circuit of two circuits transmission-lines are stand alone. The device has the ability of phase-trip that is owns transmission line single phase , 2 or 3phases fault only fault phase tripped.so as to making the power system contact as close as possible. The function of reclosing distribute into relay protection of line and breaker, so it is easy to get no-serious fault information of line protection and realize self-adapted multi pole reclosing. The breaker reclosing depend on the state of owns breaker andthe adjacent breaker. The criterion of serious fault and the sequence reclosing is simple and useful.

本项目具有如下特点:两回线的保护装置及跳、合闸回路完全独立;装置具有分相跳闸功能,即本线单相、两相、三相故障时均只跳故障相,使得故障时系统联系尽可能紧密;重合闸功能合理分配在线路保护和断路器保护中,充分利用了线路保护的信息实现无严重故障判别和分相顺序重合策略的自适应重合功能,由断路器保护根据断路器本身的工作状态和相邻断路器的配合关系实现重合出口;判断严重故障的判据及分相顺序重合闸原理简单合理,具有实用意义,降低了重合于永久故障对系统的冲击。

Turing provided an influential formalisation of the concept of the algorithm and computation with the Turing machine, formulating the now widely accepted "Turing" version of the Church Turing thesis, namely that any practical computing model has either the equivalent or a subset of the capabilities of a Turing machine.

他对计算机的重要贡献在于他提出的有限状态自动机也就是图灵机的概念,对于人工智能,它提出了重要的衡量标准&图灵测试&,如果有机器能够通过图灵测试,那他就是一个完全意义上的智能机,和人没有区别了。

The fully inseparable bright tripartite entangled state was experimentally generated using the NOPA at deamplification and the linear optical system consisting of polarization beam splitters and half wave plates. The variances of the sum of the amplitude quadratures of the three obtained optical modes and the relative phase quadratures among them is respectively 3. 28dBm and 3. 18dBm below the corresponding Shot Noise Limit.

利用运转于参量反放大状态的NOPA和偏振分束棱镜及半波片,从实验上产生了完全不可分的明亮三组份纠缠态光场,所产生的纠缠态具有三模正交振幅分量和与相对正交位相分量差的起伏方差低于散粒噪声极限的特性。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。