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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在囊胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

It is indeed a perfectly familiar fact that pleas of loss of consciousness or other abnormal mental states , or of the existence of some other excusing condition, are frequently and sometimes successfully advanced where there is no real basis for them , since the difficulties of disproof are often considerable .

这确实是一个完全相似的事实,即在不存在关于缺乏意识或其他的精神反常状态或者存在某种其他免责条件的真正的根据的场合,常常提出而且有时是成功地提出了这样的辩解理由,因为反证的困难常常相当大。

According to the complete geometric symmetry of the irons and windings, and the mirror symmetry during positive-negative operating-mode period, the expressions of current harmonics are derived, and the electromagnetic equitation are simplified. Based on this, the analytical calculation of flux density and the electromagnetic calculation of the reactor are performed.

根据两个铁心及绕组的结构完全对称以及它们工作状态在正负半周里呈镜像对称的特点,推导出电流谐波关系式,简化了本可控电抗器的电磁方程,并根据简化后的数学形式研究了其磁感应强度的解析计算,最终计算出了各项性能指标。

Our case underscores the importance of promptly evacuating an SEH.6,12,14,29 This patient presented with a severe neurological deficit with worsening paresis, and the prompt diagnosis and decompressive surgery performed within 8 hours after the onset of symptoms allowed complete recovery of her preoperative neurological deficit.

我们的病例强调了快速评估SEH的重要性。本例出现了严重的神经损伤和恶化瘫痪状态,但快速准确的诊断和出现症状8小时即施行的减压手术使得患者的术前神经损伤得到完全恢复。

All above laid a foundation for the research of polarimetric information processing and application. Then, target optimum polarization and polarimetric synthesis technique were studied. By constraining polarimetric states of transmitting and receiving antennas, co-polarized and cross-polarized signatures were obtained. Because the received wave was partially polarized, completely polarized, completely unpolarized, and total available power signatures were defined based on the power of the return wave.

接着,论文深入研究了目标最佳极化和极化合成技术,通过约束收发天线的极化状态可以得到共极化特征图和交叉极化特征图,由于雷达接收的电磁波通常是部分极化的,根据接收波的平均功率密度可以得到未极化特征图、完全极化特征图和匹配极化特征图,在极化特征图空间中搜索可以很容易地获得目标的最佳极化,这些目标最佳极化可以作为极化目标识别和分类的极化特征。

Construction of Saint-Pierre went forward fitfully under Oubrerie's supervision, but only the raw concrete of the first two administration levels and a jagged portion of the sanctuary walls had been finished by 1978, when money ran out and work ceased permanently.

圣皮埃尔教堂的建造完全在武布赫希的监督下进行,至1978年止,由于经费不够,在浇铸完两层管理用房的混泥土骨架以及圣堂的部分参差不齐的墙体后,这个项目就一直处于停工状态。

But, I will tell you, Paul: In your almost total immobilization, the visibility and tangibility of you is a bundle of infinite activity-zillions of atoms, you might say-of energy pattern being fully energized, and there is nothing sluggish about it whatsoever.

但我要告诉你,保罗,在你几乎完全不动的情况下,你有形可见的存在正以最饱合状态的能量形式(套用你们的话则是亿兆的原子)运作著,没有任何一个部分是停滞不动的。

When Ivanov concludes that"absolute equality"exists in the collective unconsciousness in"I"and"others"from Dionysiac Theory, Bakhtin's absorption and development on the basis of the dialogue, totally impels contradictions and co-existences between the carnival and the religion become inevitable after the dialogue.

当伊凡诺夫从酒神迷狂状态中总结出了&我&和&你&关系在超越个体经验的集体无意识中所具有的&绝对平等&性质时,巴赫金在对话基础上的兼收并蓄,完全使得狂欢-宗教之间的对立和共存成为对话后的必然结果。

In this market, real estate all the particularity of the product itself, together with certain existing institutional arrangements of the imperfect, which makes the market completely in monopolistic or oligopolistic structure of state.

在这个市场中,房地产的特殊性,所有的产品本身,以及一些现有的体制安排的不完善,这使市场完全垄断或寡头垄断的结构状态。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力