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With eggs and children artemia were detedcted using nested-PCR. WSSV positive products could be detected in artemia, and DNA sequence of the product accords with the designed specific amplified one according to sequence analysis. The positive rates of wild brine shrimp and filial generation were 58% and 43%, respectively. However, it seems to be no regularity between parents and filial generations positive results. It may not judge whether WSSV could vertically transmit in artemia or not.

检测结果显示:卤虫中可扩增出特异性的、与引物设计吻合的DNA片段;DNA测序分析表明,扩增片段的DNA序列与白斑综合症病毒序列一致;野生卤虫携带白斑综合症病毒的阳性率为58%,实验条件下野生卤虫的后代的阳性率为43%;同一卤虫家系中,亲代与子代携带病毒的情况不完全一致,不能确定白斑综合症病毒可否通过繁殖在卤虫世代间进行垂直传播。

ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析,40个随机引物中筛选出扩增效果较好的6个引物,每个引物能产生1~10条DNA条带,获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来,通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间,平均链锁聚类分析可将26种鹅膏菌分为二大类,且一些具菌环和苞状菌托的种类聚在一起,与形态分类基本相吻合。

Creating chorioretinal venous anastomosis potentially offers a means of permanently bypassing the site of obstruction to venous outflow, to recovery the retinal blood circulation and to reduce the retinal ischemia. It is one of the methods curing the reason of CRVO and is demonstrated effective in non-ischemic CRVO patients using laser-induced chorioretinal venous anastomosis. Patients with ischemic CRVO have more severe situations and high risk factors of later neovascular complications occuring, the surgery of via a pars plana vitrctomy combined chorioretinal venous anastomosis can be the newest attempting treatment.

其发病机理和病因目前仍未完全阐明,也没有有效的治疗措施;除常规的药物和针对新生血管并发症的视网膜光凝治疗以外,近年来开展的各种旨在重建或恢复视网膜血液循环的激光和手术治疗方法正日益成为眼科界关注的焦点;利用建立脉络膜视网膜静脉吻合途径,使被淤滞的静脉血流经由脉络膜血管流出眼外,促进或者恢复视网膜血液循环,减少视网膜缺血缺氧状态的发生,是多途径CRVO对因治疗的方法之一。

Over the past few days and watch Heling words: Luandan inside the long hair; chicken can be several feet out of 3; Yingdu on the existence of the world; dogs can be named as sheep, horse can be said to be the oviparous; can Xiama There are said to be a long tail; the fire itself does not sense heat; in the mountains echo the mountains also give birth to prove the mouth; wheels will never be a way; eyes to see as a lack of substantial capacity; to identify foreign objects can not always be the real thing; turtle May be longer than a snake; Square can not draw a square and compasses can not be used to Circle; own mortise and Suntou not completely fit; birds can be seen there had been no movement; the arrow flies off a Stay, there are not stop time; puppy dogs do not have to; Ma Huang, the Black Cattle can name three reproach; dog might as well call it the black dog; known as the lone horse that it should not have had the mother; foot Long sticks, the interception of 50 per day, 10,000 cut-off points are a never-ending, an endlessly Landiao.

而几天来观看鹤龄的言辞:卵蛋里面长着毛;鸡的脚可以数出三只;郢都内就存在着天下;狗也可命名为羊,马能够说是卵生的;虾蟆可以说是长有尾巴;火本身并没有热感;山中的回音证明大山也生出了口;车轮永远不会着地;眼睛缺乏看视实质的能力;指认外物永远达不到事物的实际;乌龟可能比蛇还长;角尺不能画出方形,圆规也不能用来画圆;自己的榫眼与榫头不会完全地吻合;飞鸟的身影也可说不曾有过移动;飞逝而去的箭头有停留、也有不曾停歇的时刻;小狗可以不是狗;黄马、黑牛的称谓可以数落出三个;白狗也可以叫它黑狗;称作孤驹应该说它不曾有过母亲;一尺长的棍棒,每天截取一半,一万年也分截不完,一篇滥调没完没了。

A shift model has been established for the first time on the basis of interlayer compensating effect of multilayer thin films, and the shift range of wavelength has been calculated using equal dividing method, the result was co incide with the measured result.

并对设计的滤光片采用对分法计算了它在低温条件下波长的漂移量,计算结果与研制出的滤光片实测结果吻合很好。并成功地将研究结果应用于滤光片的设计,对原设计结果进行了准确的修正,使得最终研制的滤光片在低温下完全满足使用要求。

The model is used to count several engineering example.Comparing the 2D numerical results with electrical simulations,it is proved that the seepage model can count the seepage surface and the location of search results in dam well and truly.Comparing 3D results with 2D results at close section,the results are tally well except the difference of meshes and the interceptive position of section.So the model is credible and has upper practicality value.In the projects,the spending of account is increasing because of the bigger account field.

将饱和—非饱和渗流模型用于几个工程实例,其二维模拟结果同渗流电模拟实验结果进行了对比,证明本文的渗流模型完全能够准确计算坝内的渗流浸润线以及逸出面位置;三维数值模拟的结果在相近剖面处与二维结果作了比较,考虑三维网格剖分及剖面截取位置的差异,三维结果与二维结果吻合的较好,证明本文的饱和—非饱和渗流模型在三维计算中是可靠的且具有较高的实用价值。

Although a great number of humoral factors have been identified, molecular mechanism of nerve regeneration after end-to-side neurorrhaphy has not been completely clarified yet.

虽然已经确认神经端侧吻合术后神经再生的大量体液因素,但是其分子机制还没有完全阐明。

The multiresolution time-domain algorithm based on the scaling function of the Daubechies wavelet is introduced, and the perfectly matched layer absorbing boundary condition for the algorithm is deduced. In order to overcome the difficulties due to the nonlocalized property of the MRTD basis functions, the pure scattered-field formulation is adopted. The method presented is applied to the scattering analysis of multiple conducting objects, and the numerical results are compared with those by the traditional finite-difference time-domain method. It is shown that the computational resource is reduced drastically without sacrificing much accuracy.

对基于Daubechies小波尺度函数的时域多分辨分析算法进行了详细论述,推导了MRTD算法的完全匹配层吸收边界条件,为克服传统MRTD方法中基函数存在着非局部性的缺点,在源的加入上采用了纯散射场方法;并应用该算法对多目标的电磁散射特性进行了分析,数值结果表明,MRTD算法与传统的时域有限差分法结果相吻合,大大节约了计算资源。

Nonetheless, I confess to being completely outmatched.

尽管如此我承认完全没有吻合的鞋样

The measurement results show that the MEMS bridge fully suspend over the signal line of CPW, do not adhere to dielectric layer and has very strong three-dimension effect under up-state. Moreover the simulation results agree with test results very well, this demonstrates that the method combining equivalent cicuit theory and simulation tool, which analyze the RF performance of RF MEMS devices, is feasible.

测试结果表明:器件射频性能的模拟值和测试结果吻合很好,证明了等效电路理论与仿真工具相结合的方法分析RF MEMS器件的可行性;器件的微结构已完全成型,立体感强,与介质层无粘连,桥本身也非常挺直。

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推荐网络例句

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。