孵育物

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

Methods: Liver microsome was preparated from normal rat and human liver, which was to he treated with a series of concentrations of CsA or coadministration with Flu or MP. The activities of CYP3A in the microsomes were detected by using erythromycin as substrate.

製备大鼠和人肝微粒体，用不同浓度的CsA作用、加或不加MP、Flu，以红霉素为底物，经过NADPH系统孵育后，利用分光光度法测定人和大鼠肝微粒体的红霉素N-脱甲基酶活性。

Methods: The health adult human primary hepatocyte microsome was randomly divided into 12 groups for control and itraconazole with different concentrations in the range of clinical drug blood concentration, each group have 10 samples. Control groups were added culture fluid, itraconazole groups were added itraconazole with different concentration respectively, after cultured 30 minutes, substrates (phenacetin for CYP1A2, testosterone for CYP3A4) were added and cultured for another 40 minutes.

采用健康成人肝细胞微粒体，分为对照组和不同浓度的伊曲康唑组，共12组，每组10个样本，伊曲康唑组分别加入血药浓度范围内不同对应浓度的伊曲康唑，对照组仅加入培养液，孵育30 min后，再加入CYP450同工酶1A2和3A4的相应底物再孵育40 min。

After that, all samples were fixed with 10 g/L paraform for 45 minutes. Then 50 μL well-fixed blood was added into the polystyrene tube, meanwhile 20 μL monoclonal antibody, such as CD42a-b-c-d and PE-labeled CD42b, was respectively mixed gently with the blood sample and incubated at room temperature in dark for 30 minutes.

取50 μL固定后全血加至流式检测管，同时分别向不同管中加入20 μL糖蛋白Ib/IX/V 复合物单克隆抗体CD42a-b-c-d和PE标记的糖蛋白Ibα单克隆抗体CD42b，混匀后室温避光孵育30 min，然后CD42a-b-c-d标记的样品中加入20 μL FITC标记的羊抗鼠 IgG，混匀后室温避光孵育30 min。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

Methods: Liver microsome was preparated from normal rat and human liver, which was to he treated with a series of concentrations of CsA or coadministration with Flu or MP. The activities of CYP3A in the microsomes were detected by using erythromycin as substrate.

制备大鼠和人肝微粒体，用不同浓度的CsA作用、加或不加MP、Flu，以红霉素为底物，经过NADPH系统孵育后，利用分光光度法测定人和大鼠肝微粒体的红霉素N-脱甲基酶活性。

The CD59 gene and MCP gene were mixed with liposome, then used to produce transgenic mice by 3 methods. Firstly, the gene/ liposome complex was directly injected into 5 male mice's testis. The 5 transfected males copulated with 25 females each 7 days later.

将分别含CD59基因和MCP基因的表达载体与脂质体混合制成基因-脂质体复合物，分别采用睾丸注射、输精管注射和精子/基因-脂质体复合物共孵育三种方法生产转双基因小鼠。

To test whether ethylene oxide retained in the dialyzers might be neurotoxic, tissue culture medium was incubated in the blood compartment of dialyzers.

如果组织培养基在透析器内孵育，然后将鼠的背根神经节（DRG培养物暴露于此培养基中，则DRG的特异性变化会发生。

METHODS: Non-derivatised cuvette immobilised Lipid A was used to track the bonding between the Lipid A and aqueous extracts and alcoholic extracts from seventy-eight TCM, and then neutralized endotoxin in vitro by selecting hypso-bonding TCM that removed the tannins.

将革兰阴性细菌的脂质A包被于生物传感器的非衍生板建立靶点，跟踪测定赤芍、大黄、黄芩等78种中药的水提物和醇提物与Lipid A的结合活性，再将筛选出的中药去鞣质后与定量的内毒素（LPS，20、50ng·ml^-1）37℃孵育30min后，测定孵育后的样品与Lipid A的结合情况，以评价样品内的活性物质含量。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。