孵化

The HE activity was almost completely inhibited by SBTI, p-APMSF, bestatin and NEM, greatly inhibited by ovomucoid, TLCK, IAM, chymostatin and PMSF, and slightly inhibited by pepstatin A, TPCK, LBTI and leupeptin. And these results imply that HE is most probably a trypsin-type serine protease.

抑制剂对其酶活性的影响结果显示，该孵化酶的活性几乎可被SBTI、p-APMSF、Bestatin和NEM所完全抑制，被ovomucoid、TLCK、IAM、chymostatin和PMSF所强烈抑制，而对pepstatin A、TPCK、LBTI和leupeptin不敏感，表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。

When the embryo reached the prehatching stage, the hatching enzyme granules were secreted into the perivitelline space from the top of HGCs.

孵化酶进入卵周液，对卵膜内层进行消化和降解，使胚胎破膜而出。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

Cooperative enterprise counterpoises except the operation that will acquire this terrace besides, still will share platform of Chinese net library and the database resource that its belong to industry net, China to take inn network permissibly, net library will plan to provide tool of package network sale for its through this hatch, it is business of the 3rd acting new-style network in order to make its grow quickly.

合作企业除将获得该平台的运营权之外，还将获准共享中国网库平台及其所属行业网、中国进店网的数据库资源，网库并将通过该孵化计划为其提供一揽子网络营销工具，以使其迅速成长为第三代新型网商。

By using red blood cell broken-up solution,proteinase K and RNase,total DNA with high quality was extracted from the egg membranes in the incubated eggshells of two pheasant species,Reeves's Pheasant Syrmaticus reevesii and Ring-necked Pheasant Phasianus colchicus from Dongzhai Nature Reserve in Henan Province.

本实验以河南董寨自然保护区白冠长尾雉和环颈雉已孵化的卵壳为材料，利用红细胞破碎液、蛋白酶K及RNA酶等试剂，对卵壳膜内的总DNA进行了提取，建立了一种提取高质量DNA的新方法。

Other nature ways to fight the fire ants are also possible. One is the South American phorid fly. It lays its eggs on fire ants. When the eggs break open, the young flies eat the brains of ants. But researchers do not know how well the flies would do in North America.

其他的自然途径来消灭火蚁也是可能的，其中之一是南美的蚤蝇，它把卵产在火蚁的卵上，当它的卵孵化时，小苍蝇就吃蚁脑，但是研究都不知道蚤蝇会对北美有什么影响。

Other natural ways to fight the fire ants are also possible. One is the South American phorid fly. It lays its eggs on fire ants. When the eggs break open, the young flies eat the brains of the ants.

另外的通过自然方式抑制火蚁的途径也可行，其中之一是南美蚤蝇，这种蚤蝇可以在火蚁身上产卵，当卵孵化后，幼蚤蝇就会吃掉火蚁的脑。

The field tests indicated that: The lowest effective dosage of the gra-nulosis virus from Pieris brassicae in the control of the small white butterfly, Artogeia rapae was in a range of 2.3 × 1010- 3.4 × 1010 GIB/mu.

田同试验结果表明：大菜粉蝶颗粒体病毒防治甘蓝地上菜粉蝶幼虫的最低有效剂量在2.3×10~(10)～3.4×10~(10)颗粒体/亩之间；病毒的喷布应在寄主卵与低龄幼虫的高峰期进行，春夏季以在田间寄主卵孵化率近20％时使用为宜。

Strong and broad expression was also observed in retina,ear,branchial arches,hatching gland,heart, pronephric duct,gut,proctodeum,pectoral fin and swim bladder.

同时Foxp1在视网膜、耳、鳃弓、孵化腺、心脏、原肾管、肠道、原肛、胸鳍和鳔等部位都有广泛的高表达。

At salinities of 20-35 ppt, eggs required 28 h, 45 h and nearly 4-5 days to develop into protozoea I at 30°C, 25°C and 20°C, respectively.

在盐度2035ppt之间，温度30、 25及20℃孵化所需时间分别为28、45小时及45天，才能变态至蚤状幼体第一期。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。