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Methods Beagle dog liver microsomes were prepared by using ultracentrifuge method. After incubating 0.4 μmolL^(-1) MN9202 with 1 gL^(-1) microsomes for 30 min at 37℃, the reaction was terminated by adding 0.5 mL alkalization. The RP-HPLC was used to determine the drug in the incubation mixture.

差速离心法制备Beagle犬肝微粒体酶，0.4μmolL^(-1)的MN9202与1.0gL^(-1)的肝微粒体酶在37℃水浴中孵育30min，加入0.5mL碱化液终止反应，然后采用RP-HPLC法测定孵育液中MN9202原形药物的浓度。

Young readers will enjoy the solid construction and humor of the story and the unstudied look and funny details in the illustrations.

在故事的高潮部分，这颗巨蛋孵出的小鸟成功地从狐狸手中拯救了那些鸡蛋孵出的小鸡们，在伟大的鸡妈妈的带领下，巨蛋找到了属于自己的新家。

By vermin, will produce at least two hundred and fifty chickens.

用这300个鸡蛋孵小鸡，这就算有坏的、生虫的，至少也能孵出250只小鸡。

Be destroyed by vermin,will produce at least two hundred and fifty

个。用这 300 个鸡蛋孵小鸡，这就算有坏的、生虫的，至少也能孵出 250

After that, all samples were fixed with 10 g/L paraform for 45 minutes. Then 50 μL well-fixed blood was added into the polystyrene tube, meanwhile 20 μL monoclonal antibody, such as CD42a-b-c-d and PE-labeled CD42b, was respectively mixed gently with the blood sample and incubated at room temperature in dark for 30 minutes.

取50 μL固定后全血加至流式检测管，同时分别向不同管中加入20 μL糖蛋白Ib/IX/V 复合物单克隆抗体CD42a-b-c-d和PE标记的糖蛋白Ibα单克隆抗体CD42b，混匀后室温避光孵育30 min，然后CD42a-b-c-d标记的样品中加入20 μL FITC标记的羊抗鼠 IgG，混匀后室温避光孵育30 min。

During the incubation of purified α-synuclein with fructose or glucose, it was observed that the protein intrinsic fluorescence at 308 nm decreased while the fluorescence of glycated derivant at 447 nm increased.

将纯化后的α-synuclein分别与果糖和葡萄糖孵育，通过内源荧光、非酶糖基化衍生物特征荧光、圆二色光谱以及电子显微镜等技术进行检测发现：α-synuclein 与还原糖共同孵育后，308 nm内源荧光强度明显降低，同时在447 nm产生一个非酶糖基化衍生物特征荧光。

Methods: By using JSB-1 with intramembranous antigen determinant as antibody, the positive rate as optimization index and MCF-7/A as model cells, cell pretreatment methods, such as the presence or Absence of FACS permeabilizing solution, the concentration of FACS permeabilizing solution, the time treating cells with FACS permeabilizing solution, the time incubating cells with JSB-1 working fluid, the time incubating cells with goat-anti-mouse IgG-FITC solution, and the conditions for keeping JSB-1 working fluid and cell numbers, were optimized.

采用抗原决定簇在细胞膜内的单抗JSB-1，以阳性百分比为优化指标，耐阿霉素人乳腺癌细胞系MCF-7/A为模型细胞，对FACS透化溶液的有无、FACS透化溶液的浓度、FACS透化溶液处理细胞的时间、鼠抗JSB-1抗体的孵育时间、羊抗鼠IgG-FITC抗体的孵育时间、JSB-1抗体工作液的保存条件、以及细胞数量进行优化。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

The nonliquefying semen from20 patients with infertility were divided into contrast group,centrifugal group and incubation group.

结果孵育组、离心组精子功能指标优于对照组（P＜0.01或0.001），孵育组精子回收率和某些指标优于离心组（P＜0.05或0.01）。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷