孵

The blots were probed with anti-Cdx-2, anti-JNK (Santa Cruz Biotechnology, Santa Cruz, California), anti-ERK, anti-phospho -ERK, antip38MAPK,anti-p-p38MAPK, anti-p-JNK (Cell Signaling, Beverly, Massachusetts) and anti-actin (Amersham Life Sciences,Piscataway, New Jersey) antibodies, and then incubated with appropriate species specific secondary antibodies labeled with horseradish peroxidase.

Blots分别与抗CDX-2抗体，抗JNK抗体（圣克鲁斯生物技术，圣克鲁斯，加州），抗ERK抗体，抗phospho -ERK抗体，抗p38MAPK抗体，抗p-p38MAPK抗体，抗p-JNK抗体（Cell Signaling， Beverly，美国马萨诸塞州）和抗actin抗体（ Amersham生命科学，皮斯卡塔韦，新泽西州）作用，然后在与辣根过氧化物酶标记的第二抗体孵育。

It is concluded that ginkgolide B can significantly affect the functions of rat polymorphonuclear leukocytes.

我们采用中性白细胞体外温孵系统，观察银杏内酯 B 对 PAF 刺激下中性白细胞功能的影响。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

The emergence of an adult insect from a pupal case or an insect larva from an egg.

孵化成虫从蛹中孵出来，或幼虫从卵中孵化出来

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次，收集的细胞用含10%胎牛血清的DMEM培养基中和，用差速贴壁分离法分离，在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The PHA stimulated CBMC which was then incubated with rIL-2 began to express IL-4 mRNA after restimulation of PMA plus iono.

将PHA刺激后的CBMC与rIL-2一起孵育，再用PMA+IONO刺激，CBMC始表达IL-4 mRNA。

Control effects of the seedlings, whorl leaves and the leaves of transgenic Bacillus thuringiensis corn on the 1 st instar, 4th instar and 5th instar larvae of Mythimna separata were evaluated in laboratory.

在室内用生测法研究了表达Cry1Ab杀虫蛋白的Bt玉米MON810和Bt11小苗和心叶对粘虫Mythimna separata初孵幼虫以及玉米叶片对暴食期4龄和5龄幼虫的杀虫效果。

Control effects of the seedlings,whorl leaves and the leaves of transgenic Bacillus thuringien- sis corn on the 1st instar,4th instar and 5th instar larvae of Mythimna separata were evaluated in laboratory.

在室内用生测法研究了表达Cry1Ab杀虫蛋白的Bt玉米MON810和Bt11小苗和心叶对粘虫Mythimna separata初孵幼虫以及玉米叶片对暴食期4龄和5龄幼虫的杀虫效果。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。