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The changes of DNA content and mitochondrial transmembrane potential in LEC were detected by flow cytometry.

将培养的牛LEC分别与160 μg/mL Ele和20 μg/mL Cur在CO2培养箱中共同育8,16,24,48,72 h,通过透射电子显微镜观察LEC超微结构;采用流式细胞术测定LEC的DNA含量及线粒体跨膜电位。

Methods The tenocytes were isolated from tendons of the Leghorn chicken with Handerson's methods.

方法按Handerson分步酶消化法分离刚出的Leghorn鸡的趾屈肌腱腱细胞。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0,5.0,10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理,设空白对照组,分别育24h、48h、72h后,收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化;免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

Like clockwork, Tiny maneuvered the ship under them as they began descending, the main entry hatch opening up.

像钟表装置,极小的 maneuvered 在他们下面的船当做他们开始降,主要的进入有话直说。

Main function: To hatch of enterprise of science and technology implement poineering contact member or advisory division execute groom; To hatch of enterprise of science and technology implement administrative layer undertakes training grooming; To general character poineering wisdom and experience undertake arrange and travelling; Brood to be in business development undertakes diagnose and advisory; Poineering adviser with man-to-man or a pair of kind of 2 are right the person that do poineering work undertakes poineering training; Oriented to construction of poineering environment of science and technology and governmental policy to table a proposal and opinion.

主要职能:对科技企业化器创业联络员或咨询师等实行培训;对科技企业化器治理层进行辅导培训;对共性创业聪明和经验进行整理和传播;对在企业发展进行诊断和咨询;创业导师以一对一或一对二的方式对创业者进行创业辅导;对科技创业环境建设和政府政策导向提出建议和意见。

They tucked it up with Marmalade to keep it warm and waited for it to hatch.

她们把她塞到咪咪的肚子下面取暖,等待小鸭子被出来。

The association is stable, and the Wolbachia strain is maternally inherited at high frequency. It is capable of inducing complete cytoplasmic incompatibility, which should facilitate its invasion into natural field populations and its persistence over time.

由于登革热病毒需要在蚊虫体内育几周的时间才能变得具有感染性,因此研究人员提出,缩短蚊虫的寿命可以有效地减缓登革热及其它相似且致命性病毒的传播。

Standard patch clamp methods were used to measure whole cell capacitance,zero current potential,reversal potential and potassium ion currents of Deiters′ cells in normal external solution.Equations were used to compute the relative membrane permeability ratio of Deiters′ cell to K+ and Na+ and the potassium equilibrium potential.

采用酶育加机械分离法分离Deiters细胞;利用膜电钳技术在全细胞模式下检测全细胞电容,记录在正常外液中的钾电流,零电流电位,反转电位,并根据相应公式计算离子通道K+与Na+的相对通透性比率及K+平衡电位。

The incubation system of chicken hepatic microsome was successfully established. The activity of cytochrome P450 was determined by ultraviolet spectrophotometry.

在建立鸡肝微粒体体外育体系的基础上,采用紫外分光光度法测定细胞色素P450酶系的活性。

Methods: Liver microsome was preparated from normal rat and human liver, which was to he treated with a series of concentrations of CsA or coadministration with Flu or MP. The activities of CYP3A in the microsomes were detected by using erythromycin as substrate.

製备大鼠和人肝微粒体,用不同浓度的CsA作用、加或不加MP、Flu,以红霉素为底物,经过NADPH系统育后,利用分光光度法测定人和大鼠肝微粒体的红霉素N-脱甲基酶活性。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。