孵

S,allowing for what may prove addle,and what may be destroyed by vermin,will p

孵小鸡，这就算有坏的、生虫的，至少也能孵出250只小鸡。

Eggs to three hundred,These eggs, allowing for what may prove addle,and what may be destroyed by

用这300 个鸡蛋孵小鸡，这就算有坏的、生虫的，至少也能孵出250 只小鸡。

These eggs,allowing for what may prove addle,and what may be destroyed by vermin

用这300个鸡蛋孵小鸡，这就算有坏的、生虫的，至少也能孵出250只小鸡。

Three hundred,These eggs,allowing for what may prove addle,and what may be destroyed by vermin,will

用这300 个鸡蛋孵小鸡，这就算有坏的、生虫的，至少也能孵出250 只小鸡。

Three hundred, These eggs, allowing for what may prove addle, and what may be destroyed by vermin, will produce at least two hundred and fifty chickens.

用这300 个鸡蛋孵小鸡，这就算有坏的、生虫的，至少也能孵出250 只小鸡。

These eggs, allowing for what may prove addle, and what may be destroyed bu vermin, will produce at least two hundred and fifty chickens.

用这300个鸡蛋孵小鸡，就算有坏的、生虫的，至少也能孵出250只小鸡。

The holding potential was -70mV.(2) By whole-cell voltage-clamp technique, after slices were preincubated with glycine receptor competitive antagonist alone or with GABA〓 receptor competitive antagonist alone or with GABA〓 receptor incompetitive antagonist alone or with GABA〓 receptor and glycine receptor competitive antagonists collaterally or with GABA〓 receptor incompetitive antagonist and glycine receptor competitive antagonist collaterally, the effects of 100μM propofol on EPSCs of the CA1 neurons of the rat hippocampus were investigated. The holding potential was -70mV.

2应用全细胞膜片钳技术，观察100M丙泊酚对单纯用甘氨酸受体竞争性拮抗剂预孵、单纯用GABA〓受体竞争性拮抗剂预孵、单纯用GABA〓受体非竞争性拮抗剂预孵、合用GABA〓受体和甘氨酸受体竞争性拮抗剂预孵以及合用GABA〓受体非竞争性拮抗剂和甘氨酸受体竞争性拮抗剂预孵的大鼠海马脑片CA1区电刺激诱发的EPSC的影响。

Glycine receptor competitive antagonist partially reversed the effects of propofol. When slices were preincubated with GABA〓 receptor and glycine receptor competitive antagonists collaterally or preincubated with GABA〓 receptor incompetitive antagonist and glycine receptor competitive antagonist collaterally, 100μM propofol still decreased the amplitude of EPSCs.

2在膜钳制电压为-70mV条件下，100M丙泊酚明显降低合用GABA〓受体和甘氨酸受体竞争性拮抗剂预孵的大鼠海马脑片CA1区电刺激诱发的EPSC幅值，下降程度小于单纯甘氨酸受体竞争性拮抗剂预孵组，大于单纯GABA〓受体竞争性拮抗剂预孵组；而且，100M丙泊酚更是明显降低合用GABA〓受体非竞争性拮抗剂和甘氨酸受体竞争性拮抗剂预孵的大鼠海马脑片CA1区电刺激诱发的EPSC幅值，下降程度甚至大于单纯甘氨酸受体竞争性拮抗剂预孵组。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

The supernatant was removed. The wells were divided into five groups with 12 wells in each group. The control group was added with 1 g/L dimethyl sulphoxide and 3.3 mmol/L or 16.7 mmol/L glucose, 1 mL per well. The four experimental groups were added with 1 mL 0.2 μmol/L, 1 μmol/L, 5 μmol/L, or 10 μmol/L clozapine apart from the above DMSO and glucose.

每12孔为一组，共5组：对照组的孵育液含1 g/L二甲基亚砜、3.3 mmol/L或16.7 mmol/L的葡萄糖液1 mL；4个不同浓度氯氮平组的孵育液除含有上述成分外，还分别含浓度为0.2，1.0，5.0或10.0μmoL/L氯氮平；各组有6孔继续孵育1 h，另外6孔继续孵育4 h；吸取上清液，保存于-20℃冰箱中待测。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷