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Knamycin and Hygromycin were taken as screening reagents for gerbera resistive plantlets, and Cefotaxime and Carbenicillin as bacteriostats. Under different concentrations, their effects on differentiation of adventive buds were different.

以卡那霉素和潮霉素作为非洲菊抗性植株的选择剂,头孢霉素和羧苄青霉素为抑菌剂,分别采取不同浓度,分析其对非洲菊试管苗不定芽分化的影响。

Heilongjiang Forest Botanical Garden, Harbin 150040, Heilongjiang, China Abstract: Using growth rate and hanging drop methods, biological activity of four acetone extracts from Sophora flavescens Ait., Galinsoga parviflora Cav., Typhonium giganteum Engl., and Datura stramonium L.

黑龙江省森林植物园,黑龙江哈尔滨 150040 摘要:采用生长速率法和悬滴法测定了苦参、牛膝菊、独角莲、蔓陀萝4种植物的丙酮提取物对水稻恶苗病菌、稻瘟病菌、玉米小斑病菌、玉米弯孢菌叶斑病菌、番茄叶霉病菌和辣椒根腐病菌的生物活性。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系:选取生长20d左右的健壮的小黑杨组培苗叶片,通过主叶脉剪2~3下,预培养3~4d;将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3~0.4浓度,进行浸染;侵染时间为6~15min;共培养3d后,转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?