孢子细胞的

By the support of the present project, 3 articles have been published, 4 articles have been submitted to Grana and Acta Botanica Yunnanica etc and some papers is in preparing.

结果表明：蕨类植物孢子外壁和周壁来源于孢子囊内层绒毡层细胞；孢子表面纹饰有的种类由外壁形成，有的种类是由周壁形成；孢子纹饰是在四分孢子形成后迅速形成的，形成的时间很短并比较稳定。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

In the late of April of the second year, male inflorescences unbound and pollinated. When female inflorescences accepted the pollen grains, ovary development was increased in early May. With the development of either of the two ovule, funiculus, integument and nucellus were differentiated. Embryo sac mother cell came into being and the meiosis begins. Then fuctional megaspore developed into matured embryo sac that contain eight nucleuses. In the end of May, double fertilization generated.

次年，4月下旬，雄花序解螺旋散粉，雌花序开花授粉；5月上旬，雌花序授粉后，迅速增长，子房膨大，两个胚珠分化出珠柄、珠被和珠心，珠心中产生孢原细胞；5月中下旬，大孢子母细胞形成并减数分裂，功能性大孢子形成成熟的八核胚囊；5月底，发生双受精。

Results indicated : The meiosis stages of megaspore of white poplars are different, while there was a association between MMCs and PMCs meiosis in the same hybrid.

研究表明，不同白杨的大孢子母细胞减数分裂进程存在差异性，而同一树种的大、小孢子母细胞减数分裂进程存在时序性相关。

To understand the degeneration of unfertilized aposporous embryo sac in an aposporous species Pennisetum squamulatum, the central cell in the unfertilized embryo sac was characterized ultrastructurally.

为理解植物无孢子生殖胚囊未受精条件下的退化，对无孢子生殖植物非洲狼尾草未受精成熟胚囊中央细胞退化做了细胞形态学研究。

Ovule development began in August, and rectangle megasporocyte began to occur in December, and linear tetrads of megaspores were formed in meiosis. In most occasions, only the chalazal megaspore was functional.

云南红豆杉胚珠从8月开始发生，大孢子母细胞12月开始发生，呈方形，减数分裂后形成4个&一&字形大孢子，在大多数情况下，只有靠合点端的大孢子发育为功能大孢子。

From a structural point of view, most of the spores of a single cell, but also some for a few cells, such as Magnaporthe grisea conidia of three cells and so on.

从结构上看，大多数孢子为单细胞，但也有的为几个细胞，如稻瘟病菌的分生孢子为3个细胞等。

Young thalluscells of low development are easy to dedifferentiate and grow intoseedings directly,while mature thallus cells have to releasemonospores to form seedings through a stage of multicellular groupfor dedifferentiation.Old thallus,mainly of productive cells,releasecarporspores to form conchocelis at last.

幼嫩的叶状体细胞，由于其分化程度低，易于脱分化而直接长成细胞苗；成熟的叶状体细胞，要经过多细胞团这样一个脱分化的阶段，然后放散单孢子成苗；而老叶状体以生殖细胞为主，只能放散果孢子，最终长成丝状体。

The results shows that the tapetal expanding abnormally occurs in dyadphase stage, microspore abortion occurs in the early of monokaryon stage, disaggregation in the end of monokaryon stage, only some spall of microspore is observed in monokaryon stage, the four cell walls also exist, the tapetal cells became completely vacuolated, almost filled the whole locule. vascular bundle of male sterile line anther vanish at mature pollen stage.

结果发现花药绒粘层在二分体时期发生异常，小孢子在单核前期开始败育，至单核后期完全解体，花药发育后期不育系药腔中只观察到少量的小孢子残体，花药壁的四层细胞依然存在，绒粘层细胞已经彻底液泡化，占据了药腔的整个空间，花粉成熟期不育系花药维管束消失。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。