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Female gametogenesis in higher plants undergoes various developmental changes, including the de novo formation of germline cells, the differentiation of the megaspore mother cell, meiosis, megaspore selection, and the development of the embryosac.

高等植物雌配子体的形成涉及孢原细胞和大孢子母细胞的确立与分化、大孢子发生、功能大孢子以及胚囊的形成和发育等多种复杂调控过程。随着当代生物技术及功能基因组学的发展,近年对雌配子体发育的研究已从细胞学描述逐渐过渡到对基因和发育调控分子机理的探索。

Cytological observation was made,which indicated that microspore degeneration was a successive process.

比较研究了可育花及两种类型的不育花小孢子发育的细胞形态学特征,发现萝卜小孢子败育是个连续过程,在造孢细胞时期就已出现败育迹象。

Aneuspory The production of an unusual number of spores during meiosis of a spore mother cell.

异数孢子:在孢子母细胞减数分裂过程中产生不正常孢子数目的现象。

Botany of or relating to the cells in a sporangium that give rise to spores.

属于孢子细胞,或与在孢子囊里提供孢子生长的细胞有关。

Trophozoite,macrogamout,microgamout, typeⅠmeront,typeⅡmeront and sporozoite in the development of C.andersoni were observed by TEM.The proliferation of C.andersoni was significantly inhibited by 1.25μg/ml NTZ and 0.31μg/ml GAs.Treated with 20μg/ml NTZ and 5μg/ml GAs respectively,the numbers of C.andersoni were only 0.29%and 0.66%compared with the untreated.

透射电镜在HCT-8细胞中观察到安氏隐孢子虫的滋养体、雌配子体、雄配子体、Ⅰ型裂殖体、Ⅱ型裂殖体和子孢子阶段。1.25μg/ml NTZ和0.31μg/ml GAs即可显著抑制HCT-8细胞中安氏隐孢子虫的增殖。20μg/ml NTZ和5μg/ml GAs作用后,虫体数量仅分别为未加药的0.29%和0.66%。

Sporulation involves an inner cell maturing into a spore and an outer cell nurturing the developing spore.

孢子的形成包括孢子内部细胞的成熟和外部细胞对形成中孢子的营养供给。

The acute toxicity andmain organs of pathological section for 30 days were observed. Result 1. Ganoderma lucidum spores oil at 0.20g/kg, 0.40g/kg, 1.20g/kg dose couldsignificantly promote the lymphocyte transformation, raise clearance index numberand phagocytic index number, enhance the activity of NK cells and the level of TNF-α, IL-2 in spleen. Ganoderma lucidum spores oil at high dose and middle dose couldsignificantly enhance the serum erythrocytolysin level.

实验结果 1低、中、高灵芝孢子油组(0.20g/kg,0.40g/kg,1.20g/kg)均可提高小鼠单核-巨噬细胞的吞噬指数及碳廓清指数,促进ConA诱发脾淋巴细胞转化,增强小鼠NK细胞活性,提高脾细胞产生TNF-α和IL-2的能力;高、中剂量灵芝孢子油可提高小鼠的血清溶血素水平。

The first split of microspore mother cell is observed after one week after beginning of temperature arised under high temperature but the split of secondary sporogenous cell doesn't be found. The split of secondary sporogenous cell is still found after one week but the first split of microspore mother cell is observed after 12d under low temperature. Formating speed of male gametophyte is affected by temperature.

在高温条件下,开始加温1周后即看到小孢子母细胞进行第一次分裂,未观察到次生造孢细胞的分裂;在低温条件下,1周后仍能看到次生造孢细胞的分裂,12d后才看到小孢子母细胞进行第一次分裂。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明,在花粉母细胞减数分裂后的四分体解离时期,不育花药的绒粘层细胞较可育花药提前开始裂解;在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象,证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

Paraffin slides observation found that the microspore of the RNMS line failed to vacuolate after its morphogenesis, disassembled and disappeared at last. After microspore morphogenesis, the breaking down of tapetum cells was delayed until microspore breading down.

石蜡切片细胞学观察,发现该隐性核不育能形成正常的四分体孢子,四分体释放出小孢子后,在单核花粉进入液泡化时小孢子的发育异常,不进行正常的液泡化,逐渐开始解体和消失;相应的绒毡层在小孢子形成后的降解延迟,绒毡层细胞的体积略有增大,在小孢子降解快结束时也很快解体。

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