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The aim of this study is to reveal the disinfectants and disinfection methods, medium components, and embryoid culture method on dissociative microspore culture.

[目的]研究消毒处理方式、培养基成分和胚状体培养方法对油菜游离小孢子培养的影响。

Objective]The aim of this study is to reveal the disinfectants and disinfection methods, medium components, and embryoid culture method on dissociative microspore culture.

[目的]研究消毒处理方式、培养基成分和胚状体培养方法对油菜游离小孢子培养的影响。

Rape have established regeneration system of cotyledons, hypocotyls, protoplasts and microspore culture.

油菜已经建立了子叶、胚轴、原生质体及小孢子培养的再生体系。

The fern spore, the original cell of the gametophyte, carries out asymmetrical mitosis to produce a large cell and a small cell. The small cell geminates to a rhizoid by tip growth, and the large cell further divides and develops to become a prothallus.

蕨类植物孢子作为配子体的原始细胞,通过不对称的有丝分裂产生一大一小两个细胞,小细胞萌发出极性生长的假根,大细胞继续分裂发育为原叶体。

Lower vascular plants having dichotomously branched sporophyte divided into aerial shoot and rhizome and lacking true roots.

低等脉管植物,有二岐的孢子体,分裂为气芽和根茎。

The results showed that the techniques could increase gametophyte adhesion rate by over 80%, made the seedlings stronger and the germination quality reach state standard of Class Ⅰ. The growth speed of clone adhesion (8~16 rows) was about 20 days faster than that of normal sporophyte seedling adhesion.

结果显示,这样的措施可使配子体附着率达到80%以上、幼苗健壮、出苗质量达到国家一类帘标准,使克隆采苗自8~16列c期间的生长发育速度较常规孢子体采苗后的生长发育速度快20 d。

Combining electron microscopy observation with DNA fluoresence detecting technique, it is systematically studied the ultrastructure of microspore, the distribution of plastid and mitochondria and the changes of organelle DNA at different developing stages in Phaseolus vulgaris, Pisum sativum, and Lilium Spec.(L. davidii and L. regale).

基于上述的情况,本研究应用电镜和DNA荧光技术系统研究了菜豆、豌豆及百合属中王百合和兰州百合这些植物种小孢子发育的超微结构,质体和线粒体的分布形式及其DNA的变化情况与遗传的关系,并应用RFLP分析了兰州百合的质体遗传方式,同时对另一种可遗传的细胞器——线粒体的遗传方式也进行了研究。

In conclusion, high genetic identities were found in sporophytic offspring derived from mono-crossing of gametophyte clones of U. pinnatifida by means of both AFLP and microsatellite markers, however, subtle genetic variations were also detected.

总之,AFLP与微卫星的分析结果一致,即配子体克隆单一交配组合的孢子体后代呈现高度遗传相似性,但也存在细微的差异。

In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors.

在交配试验中,源于不同菌株单孢分离物的菌丝原生质体的配对形成具锁状联合的菌落,而源于同一单孢分离物的菌丝原生质体的配对则形成无锁状联合的菌落,暗示担孢子中的两个核具有相同的交配型。

Hubei Shengfeng Medical Company has spent a lot of time and energy on the study of the Cordyceps sinensis and has produced a kind of similar herb. At first researchers collect funguses separated from Cordyceps sinensis that have the similar effective elements as Cordyceps sinensis. Then the funguses are inoculated on the larvae in the base. Much to the researchers' surprise, this kind of planted herb has even more nutrition and drug properties than the natural Cordyceps sinensis.

湖北圣峰药业有限公司从虫草菌天然型培养着手进行研究,从自然界采集冬虫夏草分离出来的真菌,药理功效与冬虫夏草的有效成份基本相似,接种在替代幼虫的富硒培养基上,进行固体静置培养菌丝体和分生孢子体作为有型冬虫夏草,经检测人工虫草的药性及营养成份不仅含量高於野生虫草,而且含有机硒。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。