孢子体

In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中，一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落，异核体中两种核的数量比例应该是1：1的关系；但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明，异核体菌丝两种核的数量比例可能不是1：1的关系。

The antiparasitic effect of caprylic acid against several fish parasites, i.e., the ciliate Cryptocaryon irritans, monogenean Benedenia seriolae, copepod Pseudocaligus fugu and myxosporean Kudoa shiomitsui, was examined by in vitro trials.Caprylic acid at a concentration of 1 mM had a parasiticidal effect against C. irritans theronts, B. seriolae oncomiracidia and K. shiomitsui spores and a contractile effect against B. seriolae adults, but had no clear effect against P. fugu copepodids and adults.

在体外实验中研究了辛酸对多种鱼类寄生虫的抗虫效果，如纤毛虫刺激隐核虫、单殖吸虫鰤贝尼登虫、桡足类Pseudocaligus fugu和微孢子虫库道虫。1mM的辛酸对刺激隐核虫滋养体、鰤贝尼登虫纤毛幼虫和库道虫孢子具有杀虫效果，对鰤贝尼登虫成虫有收缩效果，但对Pseudocaligus fugu的桡足幼体和成虫无明显效果。

Thress homologous chromosomes of trisomics associated as a trivalent or a bivalent and a univalent or three univalent during diakinesis and metaphase 1 ;Laggard chromosome and univalent separated in advance at anaphase I ,leading to distribution of 10-1-10,9-12,11-11,10-2-10,10-12,10-10,9-1-11 besides 10-ll;And there was distribution of 11-11-10-10, 10-10-10-12, 10-10-10-11, 10-11-11-11, 11-11-10-9, 10-10-10-10, 9-12-10-10, 9-9-11-11, 11-10-10-9, 12-10-9-11, 11-9-9-13, 10-10-10-9 at anaphase II ;n l microspore frequency were different of four trisomics.

三体在终变期和中期Ⅰ，3个同源染色体联会成三价体，或2个联会成二价体和1个单价体，或3个都为单价体；后期Ⅰ出现落后染色体或单价体提前分离，导致除10-11式分离外，还有10-1-10，9-12，11-11，10-2-10，10-12，10-10，9-1-11等不均衡分离方式；后期Ⅱ有11-11-10-10，10-10-10-12，10-10-10-11，10-11-11-11，11-11-10-9，10-10-10-10，9-12-10-10，9-9-11-11，11-10-10-9，12-10-9-11，11-9-9-13，10-10-10-9等分离方式；4个三体中的n 1小孢子频率不同。

Sinense, the spores germinate inside their exospores and then divide into massive protonemata;the massive protonemata can form two kinds of protonemata of which one is clubbed protonema with papillae,and the other is caulonema composed of long and cylindrical cells ; the gametophyte archaeocytes only occur on the massive protonemata.

结果表明：中华缩叶藓的孢子在壁内萌发，随后分裂产生块状原丝体；块状原丝体上可产生两种丝状体，一种是具疣的棒状原丝体，另一种是由长圆柱状细胞组成的轴丝体；配子体原始细胞只产生于块状原丝体上。

The main results were as follows:①The genomic DNA extraction method from TCK/TCT teliospore was established, which include cell wall lysis of teliospore by alkali solution, destroying teliospore cell wall for releasing nucleic acid from cytoplasma membrain and elimination of the PCR inhibitors such as pigments or polyphenols component.

论文主要研究结果如下：①优化了碱裂解法破冬孢子外壁-CTAB破坏原生质体，释放核酸-微量DNA过滤柱去除色素、多酚等PCR抑制物质的TCK/TCT冬孢子提取方法，排除了冬孢子细胞壁难破DNA难以提取，三甲胺、色素等PCR抑制物质导致假阴性的难题，采用此方法提取的腥黑穗菌DNA完全适用于PCR扩增。

Monosomic 3, monosomic 5, monosomic 10, trisomic 4 and trisomic 5 as experiment materials, their meiosis behavior of pollen mother cell, pollen characteristics, physiological and biochemical characteristics of anther, section observation of pollen development and the structure of embryo sac were studied, and the effects on the seed set and transmission rate were analyzed.

本文以大白菜同源四倍体小孢子培养产生的且已鉴定为3号单体、5号单体、10号单体、4号初级三体、5号初级三体的非整倍体株系及二倍体株系等为试材，对各材料的结籽率、雄配子传递率进行了统计，并对花粉母细胞减数分裂行为、花粉特性、花粉发育和胚囊结构以及花药生理生化特性等方面进行了观察研究，进而分析了其对大白菜单体n-1雄配子和三体n+1雄配子传递率的影响。

In order to raise efficiency of planting seedling by artificial spore culture of Athyrium multidentatum, in this article, spore of Athyrium multidentatum was taken as the explants, and used different categories of hormone, NaH2PO4 and AC during the period of tissue culture.

为了提高猴腿蹄盖蕨孢子人工培养成苗效率，以猴腿蹄盖蕨孢子为外植体，通过在组织培养中加入不同种类激素和NaH2PO4及活性炭，探讨了猴腿蹄盖蕨孢子萌发和成苗影响因素。

Megaspore development and abortion of"Pingyi Tiancha"Compar-ing with regular developing megaspore of normal M.baccata,the megasporemother cellof"Pingyi Tiancha"could form mono-nucleus embryo sacby perhaps irregular meiosis,then most of sexual MES depauperatedand degenerated,finally aborted,or only a few further developed.Of asexualembryo sac,one or more large cells at the chalazal end of nucellus developed in-to one to more asexual embryo sac initials.After the competition between sexu-al embryo sacand asexual embryosac,most ovules only had one asexual embryo sac matured.

平邑甜茶大孢子的发育和败育与正常发育的山定子大孢子对比，平邑甜茶大孢子母细胞经减数分裂能形成单核胚囊，之后多数有性胚囊萎缩、退化而败育，只有极少数进一步发育；而无性胚囊则从胚珠合点端1个或多个较大的珠心细脆形成并首先发育为一个或多个无性胚囊原始体；经过有性胚囊和无性胚囊以及无性胚囊和无性胚囊之间的竞争，多数胚珠只有一个无性胚囊成熟。

Nutrient cells were obtained from these pigment mutants by enzymatic method and conchocelis were got by parthenogenesis. When the conchocelis were mature and conchospores were released, they were cultured singly and picked up as F1 gametophytic thallus.

通过酶法分离突变体的营养细胞，单性生殖获得丝状体；分别使丝状体成熟并放散壳孢子，然后单株培养和筛选获得褐黄、翠绿和褐绿色子代叶状体。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。