孢子体

It was found through observing configuration of mycelium and conidiophore undermicroscope that white, diaphragmatic mycelium extended like branch, and pycnidium was roundor nearly round, and organ wall was dark brown and membranous, and spore was linear,transparent, straight or curving with 1～3 diaphragm.

于显微镜下观察菌丝体结构和分生孢子形态发现：菌丝体呈树杈状伸展，白色，有隔膜；分生孢子器球形或近球形，器壁暗褐色，膜质；分生孢子线形，无色透明，正直或弯曲，顶端略尖，1～3个隔膜。

Quantitative RT-PCR results showed that HTF1 is involved in the conidiation pathway,Htf1 mutant is not de-repressed in conidiophore development under non-conducive conditions.The observed phenotype of enhanced conidiophore development in the htf1 mutant may result from its defect in conidium development.It is possible that some feedback signal from conidia may inhibit further conidiophore development in M.grisea.

定量RT-PCR的结果也表明HTF1参与产孢信号途径。htf1突变体在不利于产孢条件下并没有去抑制分生孢子梗的发育，因此htf1突变体产生比野生型菌株数量更多的分生孢子梗可能是由于其无法形成分生孢子的原因导致的，这也似乎预示了在稻瘟病菌中很有可能存在一些来自分生孢子的反馈信号以进一步抑制分生孢子梗的发育。

The results showed that conidia were thin -walled and smooth to thick walled and verrucose; fat bodies accumulated gradually in both conidium and conidiogenous cell, and finally laid over the inner wall of conidium and disappeared in conidiogenous cell.

分生孢子发育的超微结构研究表明，分生孢子壁的发育是有个由薄而光滑到厚而有疣的过程；期间脂肪体在分生孢子和产孢细胞中不断累积，最后脂肪体沿着内壁排列成一层。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

The author first reported the social phenomenon and synchronizing development incryptosporidia,the dynamic process of the sporozoites or merozoites aparted from theirparent substances and developed into trophozoites,the culture of C.baileyi in chickenembryo,and the excysting process without both the trypsin and the sodium taurocholatein 37℃ water.The high levels of excystation(60%)oceured at 37℃ water showedthat temprature was very important in cryptosporidia excysting process.

在国内首次观察和描述了薄壁型隐孢子虫卵囊；首次报道隐孢子虫具有群体寄生现象和同步发育特征；首次描述了隐孢子虫的裂殖子或子孢子离开母体和发育为滋养体的动态变化过程；首次应用粪便中纯化的C.baileyi卵囊感染鸡胚成功，并用37℃水脱囊，其脱囊率高达60％，表明温度对隐孢子虫的脱囊具有重要作用，无需胰酶和牛胆酸钠的参与。

Female gametogenesis in higher plants undergoes various developmental changes, including the de novo formation of germline cells, the differentiation of the megaspore mother cell, meiosis, megaspore selection, and the development of the embryosac.

高等植物雌配子体的形成涉及孢原细胞和大孢子母细胞的确立与分化、大孢子发生、功能大孢子以及胚囊的形成和发育等多种复杂调控过程。随着当代生物技术及功能基因组学的发展，近年对雌配子体发育的研究已从细胞学描述逐渐过渡到对基因和发育调控分子机理的探索。

Based on the tissue culture, the effect of N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea, 6- benzyla minoprine（6-BA） and 1-Naphthylacetic acid on spore germination, protonema develop- ment and bud differentiation in Entodon challenged was investigated in this paper. The whole process was observed and taken pictures. The results show that TDZ, 6-BA and NAA have no significant effect on the spore germination. During the process of protonema development, 1.0 mg/L NAA can promote the primary development of protonema significantly, and 0.4 mg/L TDZ promotes branches formation in inter- mediate stage of the protonema development.

：研究了3种植物生长调节剂苯基噻二唑基脲、6-苄基腺嘌呤（6-BA）、萘乙酸对密叶绢藓孢子萌发、原丝体发育及芽体发生的影响，并对整个发育过程进行了显微观察和照相，结果表明：（1）3种植物生长调节剂对密叶绢藓孢子萌发影响不显著；（2）在原丝体发育阶段，1.0mg／LNAA对原丝体初期的发育促进效果显著，0.4mg／LTDZ对原丝体发育中期分枝的形成促进效果显著，6。

In order to understand the biology of apple ring spot fungi further and solve the problem of less conidiophores generated during their inducing culture in vitro, it was studied that the effect of culture medium pH, incubation temperature, light sources, and light regiment on growth of mycelia and generation of conidiophores, and the removing of mycelia on generation of conidiophores.

为进一步了解苹果轮纹病菌的生物学特性，解决其在离体培养条件下产生分生孢子很少的问题，研究了培养基pH值、培养温度、光源和光照条件对菌丝体生长和分生孢子发生的影响以及刮除菌丝体对分生孢子发生的影响。

Biological characteristics of Curvularia lunataBoedijn had been preliminarily studied. Optimal mediums for pathogen growth were PSA,PDA and OMA.For mycelia growth,sporulation and conidial germination of pathogen,optimal temperature was 25～30℃,and pH 5～9.Among the tested carbon and nitrogen resources,sucrose,glucose,carbamide,sodium nitrate,etc were beneficial for mycelia growth and sporulation.The conidia germination is strict in RH requirement:Conidia can germinate at RH 81%～98%,or have 100% germin...

香蕉大灰斑病菌适合生长的培养基有PSA、PDA和OMA；菌落生长、产孢和孢子萌发最适宜的温度为25～30℃，pH 5～9；在碳源和氮源中，蔗糖、葡萄糖、尿素、磷酸氢二氨和硝酸钠有利于病菌生长和产孢；分生孢子萌发对湿度有严格的要求，RH≥80%以上分生孢子萌发，随湿度增加萌发率增高；光照对菌丝体无显著影响，光暗交替有利于孢子的形成；孢子致死温度为55℃(10min)。

In heterosporous plants, the microspores give rise to small male prothalli bearing male sex organs, and larger female sex prothalli bearing female sex organs.

在具异形孢子的植物中，小孢子形成具小的雄性器官的雄原叶体，而大孢子形成大的具雌性器官的雌性原叶体。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。