孢子体

The spores of Neocheiropteris palmatopedate were cultured in improved Knop's medium, in-situ humus soil and red soil respectively. Spore germination and gametophyte development were observed under optical microscope and dissecting microscope, and the impact of four culture methods on the gametophyte development and sexual reproduction was compared. On these experiments, its cause of endangerment was analyzed.

采用改良Knop's固体培养基、原生境腐殖质土和红壤分别培养扇蕨孢子，光学显微镜及解剖镜下观察记录其于包子萌发及配子体发育过程，比较了3种培养方法对其配子体发育和有性繁殖的影响，并在此基础上探讨了扇蕨的濒危原因。

The fern spore, the original cell of the gametophyte, carries out asymmetrical mitosis to produce a large cell and a small cell. The small cell geminates to a rhizoid by tip growth, and the large cell further divides and develops to become a prothallus.

蕨类植物孢子作为配子体的原始细胞，通过不对称的有丝分裂产生一大一小两个细胞，小细胞萌发出极性生长的假根，大细胞继续分裂发育为原叶体。

This article reviews domestic and abroad studies on the types of spore germination,characteristics of protonema development and effects of environmental factors . It also presents the latest progress in this field from 1990s, such as the action mechanisms of Ca 2+·CAM signal system, plant hormones and light sign...

本文综述苔藓植物孢子萌发类型、原丝体发育特征以及环境影响因子等，还介绍了 2 0世纪90年代以来该领域研究的新进展，如人们以苔藓植物原丝体为实验材料进一步揭示Ca2 +·CAM信号系统、、植物激素、光信号系统等在植物体内的作用机制

The results were as follows: 1.Inoculated on the induction medium at the late uninucleate stage,the microspores could initiate embryogenesis by first divisions in both symmetric and asymmetric patterns,but the former was predominant.

通过观察玉米小孢子胚胎发生的细胞形态学特征，发现：离体培养条件下单核后期的花粉可以通过均等分裂和不均等分裂两条途径形成胚状体，与前人的研究结果一致。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好，在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用，抑制率在62.9%~85.1%；抑制孢子萌发试验表明：菌丝体甲醇提取物对烟草赤星病菌的毒力较好，其EC50仅为62.5328μg/mL；抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用，而对大肠杆菌无明显的抑制作用。

In culture, spores germinated two weeks after sowing. Gametophyte development is of the "Marattia" type. Gametophyte shape changed from circular, to cordate, to branched. Antheridia and archegonia appeared 10 and 12 months, respectively, after spores were sown.

培养时其孢子在洒播二周后开始发芽；配子体以「观音座莲舅」型发育，配子体生长过程由圆形转心脏形再形成分支。

Scanning electron microscopy showed that the spores were shrinking and collapsing, and the mycelium walls were ramified and nodulated under combined application of Na2SeO3 65.714mg/kg+LaCl3 53.002mg/kg. Furthermore the surface ornamentation of cystidia became fuzzy and disappearing, and some cystidia were disappearing and collapsing.

扫描电镜观察结果表明，与对照相比，中浓度硒镧配施（Na2SeO3 65.714mg/kg + LaCl3 53.002mg/kg）时，孢子由正常椭圆形变成全部萎缩，菌丝粗壮；囊状体在较低硒镧配施浓度（Na2SeO3 43.810mg/kg + LaCl3 35.335mg/kg）处理时表面纹饰模糊、消失，个别囊状体破裂，表面带有藤蔓状的组织。

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA，克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

Combining electron microscopy observation with DNA fluoresence detecting technique, it is systematically studied the ultrastructure of microspore, the distribution of plastid and mitochondria and the changes of organelle DNA at different developing stages in Phaseolus vulgaris, Pisum sativum, and Lilium Spec.(L. davidii and L. regale).

基于上述的情况，本研究应用电镜和DNA荧光技术系统研究了菜豆、豌豆及百合属中王百合和兰州百合这些植物种小孢子发育的超微结构，质体和线粒体的分布形式及其DNA的变化情况与遗传的关系，并应用RFLP分析了兰州百合的质体遗传方式，同时对另一种可遗传的细胞器——线粒体的遗传方式也进行了研究。

The disintegration of tapetal cell was relayed, the inner wall of tapetal cell secreted fewer Ubish bodies.

花粉败育过程中，小孢子液泡膜和细胞质膜断裂破碎，细胞质解体，线粒体、质体、内质网等细胞器解体或退化，绒毡层持续不解体，并缺少乌氏体的分泌。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。