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CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

Esophageal neoplasms ; carcinoma ; squamous cell ; lymph node status ; prognosis ; survival analysis ; postoperative treatment

食管肿瘤;癌;鳞状细胞;淋巴结状态;预后;存活率分析;术后辅助治疗

In the event either party should desire to sell, encumber, convey or otherwise dispose of or realize upon his or her Separate Property or any part or parts thereof, the other party will, upon request of a request, join in such deeds, bills of sale, mortgages, renunciations of survivorship or other rights created by law or otherwise, or other instruments, as the party desiring to sell, encumber, convey or otherwise dispose or realize upon may request and as may be necessary and appropriate to consummate the sale, encumbrance, or conveyance provided, however, that the other party shall not be obligated under this agreement to sign any agreement making his or her Separate Property subject to any debt, mortgage or encumbrance.

如果任何一方要愿意卖、质押、运送或处置或实现对自己独立的财产或任何部分或零件,对方会要求的请求,参与这项行动,法案买卖、抵押、放弃的存活率或其他权利,造成法律与否,或其他工具作为党欲出售、质押、运送或处置或认识后可以要求,并在必要和适当的完善的销售、产权、运输工具,但条件是对方不得有根据这项协议签署任何协议作出自己独立的财产受任何债务,抵押权或抵押。

The results showed that chromium trichloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF and the number of oocytes of superovulation, inhibit the germinal vesicle breakdown of mouse oocytes in vitro culture, but little impact on GVBD of mouse oocytes in vivo .

结果表明,三氯化铬可以抑制卵母细胞第一极体的释放,降低小鼠超排卵数和卵母细胞的存活率和体外受精率。

Donor maize plants grown in the fields was used as experimental materials, study of technique optimization was made mainly for improving freq. of microspore survival and ELS formation.

以大田种植的玉米供体植株为实验材料,以提高游离小孢子存活率及成胚率为重点进行了技术优化研究。

Have shown the feasibility and safety of using cryosurgery in the cirrhotic patient, but do not reveal any meaningful survival information.

在澳大利亚和美国的少量病例显示了冷冻术应用在肝硬化患者的可行性和安全性,但没有关于存活率任何有意义的证明。

The survival rate for one-year patients and grafts was 77.8% and 55.6% in CsA treated group, and 87.5% and 75% in ALG treated group, respectively.

环孢素A组和抗淋巴细胞球蛋白组人/肾1年存活率分别为77.8%/55.6%和87.5%/75%。

The patient survival rates were assessed with Kaplan-Meier curves and the chi-square test was used when appropriate.

通过Kaplan-Meier生存曲线来评价患者的存活率,适当的时候还会用到卡方检验。

PCI2 cells were treated under glucose deprivation as model group. Method Morphological and cytoflowmetric methods (the mitochondrial transmembrane potential; MEF method) were applied to evaluate the protective effect of SAB. The RNA levels of grp75 were measured by RT-PCR and immunocytochemistry were performed to investigate the expression level of PC12 cells under glucose deprivation and treated by SAB.

以PCl2细胞(大鼠肾上腺嗜铬细胞瘤细胞)的无糖培养建立细胞缺糖损伤模型,MTT法检测细胞的存活率;应用流式细胞仪检测亚二倍体细胞的比率及线粒体跨膜电位的改变;应用免疫细胞化学、RT-PCR法研究细胞内应激蛋白grp75的表达变化,激光共聚焦显微镜观察SAB作用下Ca(上标 2+)的变化。

Prolonged CIT is an independent predictor of graft survival in KT from younger donors.

CIT的延长对于年轻供肾的肾移植的移植肾存活率是独立预测因素。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力