存活率

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

They wanted to find out whether polygynous males had lower survival rates and aged faster than those of monogamous species.

他们希望发现一夫多妻制的雄性是否比一夫一妻制的雄性的存活率更低而且老得更快。

They wanted to find out whether polygynous males had lower survival rates and aged faster than those of monogamous species.

他们想看看一夫多妻制物种的雄性是不是在存活率和衰老速度上都低于和快于一夫一妻制物种。

They wanted to find out whether polygynous males had lower survival rates and aged fast er than those of monogamous species.

他们希望发现一夫多妻制的雄性是否比一夫一妻制的雄性的存活率更低而且老得更快。

They wanted to find out whether polygynous males had lower survival rates and aged faster than those of monogamous species.

他们要查明一雄多雌物种与一雌一雄物种相比，是否存活率更低，而且老得更快。

They wanted to find out whether cabal alz polygynous males had lower survival rates and aged faster than those of monogamous species.

他们想看看一夫多妻制物种的雄性是不是在存活率和衰老速度上都低于和快于一夫一妻制物种。

This paper studied the effects of ammonia-N and sulfureted hydrogen on the livability, metamorphosis rate and daily growth of Portunus trituberculatus larvae.

研究了氨氮和硫化氢对三疣梭子蟹各期幼体存活率、变态率和日生长率的影响。

Survival of the preconditioned Sca-1+ cells was 5.5-fold higher in group 3 compared with group 2 at 7 days after transplantation.

移植7天后，第3组预处理过的Sca-1+细胞与第2组相比存活率高5.5倍。

The results show that the algal viabilities after freezing can rise remarkably through controlling preculture conditions, optimizing dehydration rates and dehydration degrees and improving recovery methods after thawing.

结果表明，通过控制预培养条件，优化脱水速率和脱水程度以及改进化冻后的恢复方法等措施，可以显著地提高藻类的冰冻保存存活率。

Cell-based therapy is a promiseful option.This review will focus on the present status of cell-based therapy.Myoblast transfer therapy is hindered by minimal distribution of cells after injection,immune rejection,and poor cell survival.The drawback of bone marrow...

成肌细胞移植面临的主要限制是注射后细胞分布差，免疫排斥和细胞存活率低；骨髓干细胞移植需要解决横向分化的低效率问题；而肌源性干细胞看来能更有效地再生表达dystrophin的肌纤维。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。