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Methods Calcein-AM stained H9/HIV-1 ⅢB cells were treated with difierent concentrations of PF in the first,second and third trimesters respectively,which was then mixed with MT2 cells and put under fluorescent microscopy for detection of HIV-1-mediated syncytium formation.MT2 cells were treated with cell-free HIV-1 Ⅲ B,and then washed and cultured with different concentrations of PF in the first,second and third trimesters respectively.Furthcrly,the protecting effect of PFs against HIV-1-infected cells Was determined with MTT,viral replication Was evaluated by measurement of the levels of p24 antigen in culture supernatants with ELISA and the inhibition rate Was calculated.

采用荧光染料Calcien-AM标记的H9/HIV-1 ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后,与MT2细胞混合培养,荧光显微镜下观察合胞体的形成;用游离的HIV-1 ⅢB感染MT2细胞,并分别与早、中、晚孕期不同稀释浓度的PF作用后,用MTT法检测HW-1感染细胞的存活率,用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。

When Milk Thistle seeds are used within 48 hours, the survival rate is almost 100%.

水飞蓟的种子在48小时内,存活率仍是100%。

My method of the \\"triangle of life.\\"

的人能够达到100%的存活率

Of the "triangle of life."

够达到100%的存活率

People using my method of the 'triangle of life.

角的人能够达到100%的存活率

Methods PMSG-HCG were injected into 5 weeks SD rats. Embryos were collected by tearing intumescences of oviduct or clysising fimbriae tubae. The amount of embryos, rates of fertilization, and survival rate of embryos after injection were analyzed to compare the two methods.

选用最佳超排5周龄SD大鼠,腹腔注射PMSG-HCG,分别用撕开输卵管壶腹部和输卵管伞端冲洗两种方法采集胚胎,比较获得卵数,受精率,注射存活率等的差异。

Background: To assess long-term survival in unselected patients with coronary artery disease in who an invasive approach is considered.

背景:评估考虑行侵入治疗的未加选择冠心病患者的长期存活率

Background Vasopressin is an alternative to epinephrine for vasopressor therapy during cardiopulmonary resuscitation, but clinical experience with this treatment has been limited.

尚难确定。有研究发现,与室颤及无脉性电活动患者的研究结果不同,心脏停搏患者接受加压素治疗后入院存活率比接受肾上腺素治疗者高4JD%。

Nutrition of weanling pigs remains a key topic of interest in pig production because a good start in the post-weaning period is critical in the subsequent growth, development and survival of pigs through to market.

断奶仔猪的营养仍然是一项与养猪生产相关的关键问题,因为断奶后阶段良好的开端对仔猪此后直到上市前的生长速度、身体发育和存活率都非常关键。

It is significance to further study the tolerance mechanism ofHeilongjiang wood frog during hibernation. Its cold-tolerant mechanism has an importantapplied value and a great potential for exploitation and utilization.We choosed Heilongjiang wood frogs from Yichun, Heilongjiang province.

本试验选取黑龙江省伊春地区黑龙江林蛙为研究材料,观察黑龙江林蛙在环境温度为室温(14℃)、1℃和-2℃条件下的存活率,并采用2-D native-PAGE/SDS-PAGE电泳、酶活性比色法、同工酶电泳等生化技术检测不同温度环境下肝脏蛋白质及代谢酶的活性变化。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力