存活率

Methods Morphologic change of cells were observed with fluorescence microscope;MTT assay was used to evaluate survival rates Survival rates of GLC-82 cells;The influence of NA to cell migration was analyzed by scarification test; The percentage changes of apoptosis and the distributional percentage of cell cycle were analyzed with flowcytometry.

荧光显微镜观察细胞形态的变化；MTT 法检测不同浓度烟酰胺培养细胞后细胞的存活率；划痕试验检测不同浓度的烟酰胺对细胞愈合力的影响；流式细胞仪检测细胞周期和凋亡率。

Resultsshowed that 0.2%～0.5% sea snail enzyme powder,20℃～25℃,acidcondition(pH=6.5),4 hours hydrolysis can obtain large amount ofcells with highest viability.

结果表明：0.2％～0.5％的海螺酶粉，20℃～25℃，在弱酸性条件下，4h或2～3h，可以获得较高的细胞存活率。

Based on the established GIS of the Oncomcelania Snails' distribution in Dongting Lake, the author analyzes the environmental condition of the Oncomcelania Snails' habitates comprehensively by the spatial analyzing technology. The results are as following: firstly, the predominant vegetation of Oncomcelania Snails habitat is reed and sedgy. The habitats with reed has a higher livability than with sedgy as dominating vegetation. However, the diffusion of Oncomcelania Snails is much serious in the habitats with sedgy. Secondly ,the predominant soil type of the Oncomcelania Snails breeding area is temporary submergic paddy soils, which is in weak acidity environment, of that PH value is 5.5--7.5, and the organic content is in 2---3%. Thirdly, the 80 percent of the Oncomcelania Snails breeding area, the normalized difference vegetation index of that is in 0.100-0.375%, and the soil humidity of that is in 0.202—0.507, and the distance to the water of that is in 10-230 meters.

在已建立的洞庭湖区江滩钉螺分布地理信息系统的基础上，运用GIS空间分析技术，对钉螺孳生地的环境状况作了比较全面的分析，发现洞庭湖区江滩钉螺孳生地植被类型主要为芦苇和莎草类，钉螺在莎草地带中的存活率高于芦苇地带，而钉螺感染情况最严重的在芦苇地带中；孳生地的土壤类型主要为潴育性水稻土；孳生地土壤PH值处于5.5-7.5之间的弱酸性环境，有机质含量处于2-3%之间；80%孳生地的植被标化指数在0.100-0.375之间、土壤湿度在0.202-0.507之间、近水源距离为10-230之间。

To ensure the survival rate of seedlings, it is necessary to keep a certain distance among them.

为了保证幼苗的存活率，保持一定的苗距是必要的。

In the spring of 2002, 11 different shell colors lines including 4 orange ,3 purple and 4 white were successfully established by using the mate strategy of self-fertilization.

利用壳色可遗传的特性，采用自体受精的交配策略，于2002年成功地建立了4个橙色、3个紫色、4个白色等3类共11个海湾扇贝自交系，并对不同家系的受精卵大小、胚胎孵化率及幼虫、稚贝和养成阶段的生长速率与存活率等生物学参数进行了比较。

The cells were treated with taxol or/and radiation. Growth curves were drawn and sensitizer enhancement ratios were evaluated.

2细胞经紫杉醇和照射处理后，绘制细胞生长曲线，计算出10%存活率时的放射增敏比。

Theexperimental results were as follows: 1. The effects of prey density (0.1, 0.3, 1, 3 and 10 ind/mL) on growth and survivalin different body sizes (0.68, 1.50, 3.20 cm) of hybrid snakehead larval were investigated.

本实验针对杂交鳢苗种培育存活率普遍偏低的状况，对影响其生长、存活的饵料密度、放养密度和残食三个关键因素做了深入研究，试验结果如下： 1。

Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法］对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后，分别采用MTT试验、蛋白印迹技术和逆转录－聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

On the media containing 0.02 and 0.04 g/L potassium sorbate, the survival rates of explants were 13% and 3% resp. On the other media, all the explants died.

在含0.02和0.04 g/L山梨酸钾的培养基上外植体的存活率分别为13%和3%，其余培养基上的外植体全部死亡。

As a result, commercial banks in the current survival rate of space would be greatly reduced, resulting in difficulties in banking operations.

其结果是，商业银行在目前的存活率空间将大为减少，造成困难的银行业务。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。