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The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物,且所有试材的扩增结果一致,均无目的条带以外的产物扩增,说明Ty1-copia类逆转座子在苹果属植物中广泛存在,且存在历史可能较为久远;从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066),结果表明,同一基因组来源的这些序列存在高度的异质性。

There were prerenal fascia posterolateral to the left mesocolon, preaortic fascia median to the left mesocolon, parietal layer of the pelvic fascia posterolateral to the mesorectum, which continued at different sites.

左半结肠系膜后外侧存在肾前筋膜;中线侧存在主动脉前筋膜;直肠系膜后外侧存在盆筋膜壁层。这三个部位的筋膜相互延续。

The misrecognition of the performative dimension runs here in the opposite direction: the classical Master is the Master only insofar as his subjects are treating him as a Master, but here, the People are the "real People" only insofar as they are embodied in its representative, the Party and its Leader.

在此,基本的欺骗存在于这样的事实中,即这个领袖的参照点,对于他正向其提到要合法化他的统治的情况是不存在的,或者更准确地说,这只有通过其物神崇拜的代表--党及其领袖--并在其物神崇拜的代表中才是存在的。

In this article the indirect and direct evidence for the presence of nanobubbles and the basic properties of nanobubbles, including its formation mechanism, morphography and distribution, are reviewed. And the influence of nanobubbles on the long-rang hydrophobic interaction, fluid slippage and some biological problems is discussed.

本文综述了从提出纳米气泡存在一直到实验证明的过程、纳米气泡的形成机制和形貌、分布特征等基本性质以及纳米气泡的存在对疏水长程作用和流体滑移的影响,并阐述了生物学中一些与纳米气泡存在有关的问题。

RESULT ①Two different ITS2 sequences (GenBank accession number: AF416783, AF416784) and D3 sequences (AF416782, AF415594), named as An. minimus A and An. minimus C, were detected in this study. They differed by 5. 8% in the ITS2 region with 22 fixed nucleotide substitutions and indels, and by 1. 5% in D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An.

结果 ①分别发现两种不同的ITS2序列(GeneBank登录号:AF416783,AF416784)和D3序列(GeneBank登录号:AF416782,AF425594),与微小按蚊A和C同源。A、C两者ITS2序列22个固定位点存在碱基置换和插入/缺失,序列差异为4.8%;D3基因存在5个固定点突变,突变率1.5%。mtDNA-COII基因存在个体差异,但远小于同系其它成员如乌头按蚊、杰普尔按蚊间的变异。

Moreover, we examine how the agency cost between managers and liquid shareholders is affected by nonliquid shares and stateowned shares, which we believed to exist only in Chinese Market. Through the same method of event study, we find a symmetric effect regardless of the proportion of nonliquid shares, and this shows that the proportion of nonliquid shares is not a factor of conflicts between managers and liquid shareholders. As for the sub-sample with stateowned shares, there is a larger positive price impact for firms with high proportion of stateowned shares, which tells us that the stateowned shares do raise the problem of "absence of the owner", and it is a part of agency cost in China.

我们进一步检验了国内特殊存在的非流通股以及国有股对于管理层与流通股东之间代理成本的影响,通过同样的事件研究方法对上述样本进行市场宣告反应检验,结果发现非流通股比例不同的公司其市场反应并无显著差异,说明非流通股的存在对管理层与流通股东之间的代理问题没有影响;而国有股比例不同的公司其市场宣告反应存在显著差异,这说明国有股产生的&所有者缺位&问题成为国内代理成本的一部分,并确实影响了现金股利的发放。

The first of the North American advocates of free trade, as understood by Adam Smith -- Thomas Cooper, President of Columbia College -- denies even the existence of nationality; he calls the nation 'a grammatical invention,' created only to save periphrases,a nonentity, which has no actual existence save in the heads of politicians.

就亚当·斯密所了解的,美国倡议自由贸易最早的是哥伦比亚学院院长托马斯·库柏,他甚至否认国家的存在,把国家说成是出于&字面上的创造&,只是为了避免表达时的多所周折而设的,除了存在于政治家的头脑以外,并没有实际的存在

This section firstly carries on a brand-new limits to the labor POBS,and determines the scope of should protecting benefit of enterprise,which helps to solve the most major problem of abusing.It also established several major principles of legislate of POBS,including equal rule,benefit balanced principle,reasonable limit principle, value compensation principle,social public interest first principle,the reason of leaving job not to affect the provision of POBS principle and so on.It carries on standard in principle to the system of POBS and refinement stipulations taking the principle as the direction.It also restructures the system of POBS,making the system Concrete,unitizing and operational from two major angles of on-job POBS and quit-job POBS.

第一小节在第一、二部分的基础上详细分析了我国劳动者竞业禁止制度所存在的几大问题,这几大问题包括:对竞业禁止的概念及适用条件规定不明,提供了滥用的可能性、立法之间存在冲突、立法过于原则、缺乏可操作性等;第二小节,针对我国劳动者竞业禁止制度存在的问题提出建议,首先对劳动者竞业禁止统一进行了全新的界定,确了企业应保护利益的范围,解决了竞业禁止制度被滥用的最大问题;确立了劳动者竞业禁止立法的几大原则,包括平等原则、利益平衡原则、合理限制原则、对价补偿原则、社会公共利益优先原则、离职原因不影响竞业禁止条款的原则等,对劳动者竞业禁止制度进行原则上的规范并以原则为指引对该制度将涉及的某些内容进行了细化的规定;对劳动者竞业禁止制度体系进行重构,从在职竞业禁止、离职竞业禁止两大角度将制度具体化、统一化、操作化,此部分旨在为完善我国立法提供参考。

The results show that: there were significant difference on the honesty of university students with different grade; there existedsignificant difference on the parental rearing styles of university students with different sex; there existed great correlation between the parental rearing styles and honesty; father's refusal, denial and favoritism had negative predictation on honesty at some degree.

结果表明:不同年级的大学生在诚信上存在显著差异;不同性别的大学生的父母教养方式存在显著差异;父母教养方式与诚信存在着较高的相关;父亲的拒绝否认、偏爱被试对诚信有一定的负向预测作用。

Effect of protein and lipid in rice on starch physicochemical characteristics, difference between the region of non-chalkiness and chalkiness of rice, dynamic variation of cell structure and CaM distribution on rice spike were expounded in this study. It was found that protein and lipid in rice had important effect on starch gelatinization, there were distinct differences between the region of non-chalkiness and chalkiness of rice, vascular bundle system in rice spike was changed during grain filling, difference quality of different position of rice spike was more distinct in dense spike rice for more powerful vascular bundle system, the development of rice seeds was corresponded to pericarp development, difference quality of different position of rice spike was also related to transport function of rachilla besides activity of "source", and calmodulin relative activity was closely related to grain filling velocity and enginery properties.

通过实验发现:稻米中蛋白质、脂肪对淀粉的糊化过程有着较大的影响;精米的非垩白与垩白部位以及心白与腹白部位之间在蛋白质、氨基酸含量及组成方面存在着明显差异:水稻穗部大维管束面积以及主穗轴维管束数目在灌浆过程中存在明显的动态变化趋势;密穗型水稻强势粒的维管组织比散穗型水稻发达,致使其强、弱势粒维管组织差异明显加大;果皮的发育与籽粒发育状况是相适应的:强势粒和弱势粒在灌浆生理方面的差异,不仅表现在籽粒本身的&库活性&上,而且也与其着生小穗轴的输导功能有关;CaM相对活性与水稻灌浆速率、机能特征间存在着密切联系。

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推荐网络例句

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。