孕酮

(1) to deliver the Levonorgestrel (0.ml/kg,5mg/m) and Mifepristone gelatum (0.1ml/kg,5mg/ml) per cutem to the rat respectively and measure the serum concentration of levonorgestrel、mifepristone、estradiol and progesterone at different time by the means of HPLC, radioimmunoassay,chemical separation and atomic absorption spectrophotome.

大鼠皮肤涂抹米非司酮凝胶(0.1ml/kg，5mg/ml)和左炔诺孕酮凝胶(0.1ml/kg，1mg/ml)后的不同时间，利用高效液相色谱法、放射免疫分析法、化学分离与原子吸收分光光度法，测量血清中米非司酮浓度、左炔诺孕酮凝胶以及血清雌二醇、孕酮浓度。

GnRH could markedly suppress progesterone secretion of cultured GCs with or without FSH and LH co-treatments. E〓 could invert the suppression of GnRH completely, also had the additive action on secreting progesterone with GnRH co-treatment. Mel partially released the suppression action of GnRH.

GnRH能强烈地抑制体外培养的颗粒细胞分泌孕酮，其抑制作用几乎不受FSH和LH影响；Mel仅表现部分地缓解了GnRH的抑制效应，但没有逆转。E〓不仅可以完全逆转GnRH的抑制作用，进而与GnRH叠加促进颗粒细胞分泌孕酮。

Objective To study effects of progesterone on the expression of bcl-2 gene in human uterine leiomyoma cells.

目的 观察孕酮、左炔诺孕酮和醋酸甲羟孕酮对体外培养子宫肌瘤细胞bcl-2基因表达的影响。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

168 cases, who were pregnant for 5 to 10 weeks, were divided into 3 groups and the serous progestin was tested by chemoluminescence method to judge whether the premonitory abortion was successful or not.

选取孕5周～10周共168例，将病人分为3组，用化学发光法测定血清孕酮值，根据血清孕酮值来判断先兆流产成功与否的依据。

METHODS: The pregnant mice were administered by oral gavage with MXC at different doses of 0, 20, 100 and 200 mg/kg during day 12-17 of pregnancy. Meanwhile, other pregnant mice as a control group were gavaged sesame oil solvent in the same manner. The changes in body mass and number of corpora lutea of the pregnant mice were observed; and the body mass and anogenital distance of their F1 foetuses were recorded, too. The concentrations of estradiol (E2 ) and progesterone in sera of the pregnant mice were determined using radioimmunoassay.

将交配成功的雌鼠于孕12～17 d每日给予不同剂量的MXC(0，20，100，200 mg/kg)灌胃，并设芝麻油溶剂为对照组，观察各组孕鼠体质量和黄体等计数测定，同时观察各组F1子代仔鼠的体质量和肛门生殖器距离等，采用放射免疫法检测孕鼠血清雌二醇(E2)和孕酮含量。

The results showed that the main pathogenic bacteria ledlocal cows to endometritis were streptococcus,the next,Escherichia coli,staphylococcus and pseudomonas aeruginosa.NFPL could quickly reduce thenumber of the bacteria in uterus,and strongerly inhibit these bacteria in vitro.NFPL could excit the uterus,which occurred in enlarging contractionamplitude.NFPL could lower abnormal high content of progesterone and raiseabnormal low oestradiol.

结果表明，引起本地奶牛子宫内膜炎的主要致病菌是链球菌，其次是大肠埃希氏杆菌，葡萄球菌，绿脓杆菌；新促孕液能能迅速减少病牛子宫内细菌的数量，在体外对这些细菌也有较强的抑制作用；新促孕液对兔子宫有明显的兴奋性作用，主要表现在收缩波幅的加大；新促孕液能降低病牛血清异常升高的孕酮含量，升高异常降低的雌二醇含量。

To observe the morphologic changes of deciduas and progesterone receptor expression in deciduas of early unexplained spontaneous abortion patient with immunohistochemistry staining.

同时，在体外培养细胞时，再加孕酮刺激的为P组，未加孕酮刺激的为NP组，用于观察孕酮在不明原因自然流产时对患者PBMC产生细胞因子的作用。

The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17 b -estradiol, testosterone and progesterone antibodies.

在卵巢发育早期，卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应；在大生长期和成熟期，卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱，而对雌二醇则显著增强。

A liquid chromatography-mass spectrometrymethod for determination of 15 anabolic steroids(17β-TS, PG, 17β-NT, MTS, PTS, PN, PNT, DIA, BOL, TRE, STA, TRA, MED, NOG, MPA) in animal urines was established. After the treatment of enzyme hydrolysis, the sample was cleaned up by C18 solid-phase extraction cartridges. The elution was evaporated under nitrogen and redissolved by acetontrile-water(1:1, by volume).

采用液相色谱－电喷雾串联质谱仪(LC-ESIMS/MS)，在多反应监测模式下建立了动物尿液中15种甾类同化激素（睾酮、孕酮、诺龙、甲基睾酮、丙酸睾酮、丙酸诺龙、苯丙酸诺龙、大力补、勃地龙、群勃龙、康力龙、醋酸群勃龙、甲炔诺酮、甲羟孕酮、醋酸甲羟孕酮）的快速确证测定方法。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。