孕牛

In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿，死后2h内无菌条件下摘取眼球，剥取视网膜组织，混合酶消化法培养人视网膜Müller细胞；取健康新西兰大白兔，死后以最快的速度无菌条件下摘取眼球，组织块贴壁法培养兔视网膜Müller细胞；利用含胎牛血清的高糖DMEM作为培养液，不添加生长因子。

Methods: The mesencephalon of rat with conceptus age of 13 days was taken out and dissociated into single cell suspension mechanically, and then cultured in serum-free medium. Fetal bovine serum was used to induce cell differentiation. The isolated cells and their progeny were observed by using immunofluorescence technique.

取孕14 d胎鼠大脑皮层，吸管吹打机械分离制成单细胞悬液，采用无血清培养和胎牛血清诱导分化，应用免疫荧光技术鉴定神经干细胞及其分化的子代细胞。

Using progesterone and bovine serum albumin as model compounds respectively, fluorescent labeled small molecule hapten and large molecule antigen were prepared.

1以孕酮和牛血清白蛋白为模型化合物，制备了小分子半抗原和大分子抗原的荧光标记物，研究了标记物的抗原活性和光谱性质，结果表明标记物是有效的毛细管电泳免疫分析试剂；制备了抗体F〓片段，研究了其抗体活性，结果表明F〓片段比整个抗体更适合作为毛细管电泳的免疫分析试剂。

The testing methods and targets were shown as below:Recorded milk weights from the 7th day postpartum to 15th day postpartum,and calculate the average day-milk yield;Milk samples were draw in 7th day and 14th day postpartum,and testing the milk fat,milk lactose,milk protein,milk Zn and the milk somatic cell counts;Blood samples were adopted at the 7th day postpartum,testing the levels of serum PRL; Blood samples adopted at the 1st day and the 12th day in oestrous cycle were used to test the levels of E_2 and P_4 in serum;In the experimental stage, registering the condition of parturition, the mean birth weight of calves,the first oestrus,the oestrus scycle postpartum and the conception rate of the first service after artificial insemination.

试验方法及所测指标如下：于产后第七天到第十四天记录日产奶量，最后计算每组平均日产奶量；每组随机抽取分娩后第七天、第十四天的奶样，测定乳糖、乳蛋白、乳脂、乳锌含量及乳体细胞数；于产后第七天，随机采取奶牛血样，测定血清中PRL含量；于发情周期的当天和第12天，随机采取血样，测定血清中雌二醇(Estradiol，E2)和孕酮(Progesterone，P)的浓度；记录奶牛的分娩情况、犊牛初生重、产后第一次发情日期、产后发情周期，并采用人工授精技术进行受精，记录第一情期受胎率，最后取平均值，作为衡量奶牛繁殖性能的指标。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

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车载式挤奶机电机柴油动力铡草机 TMR全日混粮饲料搅拌车挤奶机真空泵供应商玉牛活塞式挤奶机，真空泵式挤奶机，提桶式挤奶机，管道式挤奶机，并列式挤奶机，鱼骨式挤奶设备，机械半自动脱落式，电子计量全自动脱落式，计量计式，计量瓶式成套挤奶厅设备，风力扬水机，大棚卷帘机，青贮铡草机，单盘，双盘割草机，揉搓粉碎机，液压打捆机，进口干式，水环，油环真空泵，并提供挤奶厅设备专用清洗液，乳头消毒润肤液，乳房炎测定仪，卡玛早孕试纸，牛奶过滤纸，犊牛喂奶器，药浴杯，真空泵油，修踢套装，牧场管理软件等，代理销售国际知名品牌成套挤奶设备配件：1、Interpuls全部产品：L02脉动器、L80脉动器、LE20电子脉动器、真空调节器、自动脱落ACR、自动清洗、脉动器测试仪、奶衬、奶衬、双脉动管等等。

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车载式挤奶机柴油动力铡草机 TMR全日混粮饲料搅拌车挤奶机真空泵供应商玉牛活塞式挤奶机，真空泵式挤奶机，提桶式挤奶机，管道式挤奶机，并列式挤奶机，鱼骨式挤奶设备，机械半自动脱落式，电子计量全自动脱落式，计量计式，计量瓶式成套挤奶厅设备，风力扬水机，大棚卷帘机，青贮铡草机，单盘，双盘割草机，揉搓粉碎机，液压打捆机，进口干式，水环，油环真空泵，并提供挤奶厅设备专用清洗液，乳头消毒润肤液，乳房炎测定仪，卡玛早孕试纸，牛奶过滤纸，犊牛喂奶器，药浴杯，真空泵油，修踢套装，牧场管理软件等，代理销售国际知名品牌成套挤奶设备配件：1、Interpuls全部产品：L02脉动器、L80脉动器、LE20电子脉动器、真空调节器、自动脱落ACR、自动清洗、脉动器测试仪、奶衬、奶衬、双脉动管等等。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。