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Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6/ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片,分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化,即方法1先用含bFGF的培养基进行预诱导,之后再用含叔丁基对羟基茴香醚,和二甲亚砜的无血清培养基进行诱导;方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导,然后不同浓度的上述物质进行正式诱导。

In order to ascertain the causes and mechanisms of corrosion and abrasion failure,the demonstration analyses and experiments such as macro/microscopy and corrosion substance composition analyses were carried out on the broken section of the man-hole door of refeed valve and air-hood .

重点对返料阀入孔门盖及风帽吹气管断口的宏微观形貌、微区成分、腐蚀物组成等进行表征分析和试验,确定腐蚀磨损产生的原因及腐蚀机理;最后根据实际状况,提出相应的预防措施和建议。

One is that,by the shove of passive rift valley polycycle,quartz sandstone with high maturity of lithologic composition deposits on the background of sub-plain during sag period;together with the rock formation environment of paranormal saltless local water,the reservoir characterized b.

厘定该盆地这种突出商业价值的地质因素主要表现在以下两个方面:1被动裂谷的多旋回推移,在坳陷期的准平原背景下沉积了高岩石成分成熟度的石英砂岩,加之超淡地层水的成岩环境,最终形成盆地的高孔高渗储层;2&教科书&式的生储盖组合。

At the top of the quartz crucible, there was a baffle ofdiameter 2mm which make the vaporing rate of the evaporator source steady.

在石英坩埚顶口处盖上开有直径约为2mm蒸发孔的挡板,提高了蒸发源加热时的蒸发速度的稳定性。

Then cover its opening with the lotus lid cut off earlier and fix it with toothpicks.

将浸透之糯米灌入藕孔内,然后盖上,以牙签固定藕盖。

The gear mentions and the position of intermediate propeller shaft is decided by the means " computation, mapping and tries to collect many times". Calculates the coordinates of the spindle and drive shaft and checking the center distance, determined department spindle must use dislodges the gear.

本课题设计的组合机床采用&一面两销&定位、液动夹紧,一次装夹加工柴油机齿轮室盖三个面上的孔,保证了加工精度,提高了生产效率,减少了工人的劳动强度。

Calculates the coordinates of the spindle and drive shaft and checking the center distance, determined department spindle must use dislodges the gear.

根据柴油机齿轮室盖的结构特点、加工部位、尺寸精度、表面粗糙度及生产率等要求,确定该机床为单工位卧式组合机床;为确保加工精度,采用&一面两销&的定位方案;为实现无级调速,安全可靠,选择液压滑台;根据零件的大小及被加工孔的位置确定主轴箱的轮廓尺寸;由加工工艺选择滚珠轴承主轴,通过计算扭矩确定主轴和传动轴的直径;齿轮的模数是通过类比法确定;齿轮齿数和中间传动轴的位置是由&计算、作图和多次试凑&相结合的办法确定;计算主轴、传动轴的坐标并进行中心距验算,确定部分轴上需采用变位齿轮;轴上的齿轮套、键等零件按轴号选取相应的标准件。

The whole divides into four parts: The furnace stack is composed of the square edged orifice copper cash; Binaural by the shovel, the knife-shaped copper coin confronts; The tower covers by the silver piece superimposes; Legs by pearl skeleton.

整体分为四个部分:炉身由方孔铜币组成;双耳由铲、刀币对峙;塔盖由元宝叠加;鼎足由珍珠构架。

Trim and devein prawns. Wash and wipe. Divide into halves lenghwise along the back. Arrange on plate with the tails at the centre. sprinkle with pepper and 1/3 tsp of mung bean flour. Pour 1 tbsp of water. Cover with microwave-safe plastic wrap.

虾剪去须及脚﹐抽去肠﹐洗净抹乾水﹐从背部切开边成二片﹐排在碟上﹐虾头向碟边﹐洒下少许胡椒粉﹐把 1/3茶匙生粉平均洒在虾上﹐淋下一汤匙水﹐盖上微波炉用保鲜纸﹐留一孔让蒸气排出。

In this design introduced the transmission covers the punch holes the ramming craft analysis, the craft plan formulation, arranges the specimen map design, the always ramming strength computation and the center of pressure computation, the cutting edge size computation, the spring, the rubber computation and selects, the raised mold, the concave mold or the convex-concave mold structural design and all that die the components structural design, draws up the mold assembly drawing and the work detail drawing, the compilation design instruction booklet, fills in the ramming craft card and the work components machine-finishing technological process card, raised pays attention to, the concave mold coordinate processing.

本设计中介绍了传动盖冲孔的冲压工艺分析,工艺方案的分析和制定,排样图的设计,总的冲压力计算及压力中心的计算,刃口尺寸的计算,弹簧的计算和选用,凸模、凹模或凸凹模结构设计以及其他冲模零件的结构设计,绘制模具装配图和工作零件图,编写设计说明书,填写冲压工艺卡和工作零件机械加工工艺过程卡,注意凸、凹模的配合加工。

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推荐网络例句

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。