孔盖

METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10^8/L were incubated in 12-well plate with a coverslip.

全骨髓法体外分离培养、纯化大鼠骨髓间充质干细胞，传至第4代时，按4×10^8 L^(-1)密度接种于置有盖玻片的12孔板内，制备细胞爬片。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

He had aimed at Hector's head near the top of his helmet, but bronze was turned by bronze, and Hector was untouched, for the spear was stayed by the visored helm made with three plates of metal, which Phoebus Apollo had given him.

但是，铜枪击中铜盔，被顶了回来，不曾擦着鲜亮的皮肤：盔盖抵住了枪矛――这顶头盔，三层，带着孔眼，福伊波斯·阿波罗的赠品。

When the gland flange bolts are tightened and the seal stem packing is compressed and valve cap hole.

当压盖法兰螺栓已拧紧，填料被压缩并密封阀杆和阀帽孔。

Demolition of the roof installation holes trough the rain cover - a fixed mounting brackets - Fixed luggage rack - tighten the screws - plastic lid cover.

拆卸车顶雨槽安装孔位盖子--固定安装支架--固定行李架--拧紧固定螺丝--盖好塑料盖子。

The edge of the door Inside the door Locked up in the first set, the installation of the first lock, the first fixed-locking screw, chip out the door switch on the surface for a long 7MM Outside the door The switch will lock the first film are locked out of the hole switch, bear in the head and tail of the screw will be fixed lock body, to install locks are locked before and after the place to build.

门边缘 门里面套上锁头，安装锁头，上紧锁头固定螺丝，开关片伸出门表面长为7MM 门外面将锁头的开关片伸出锁身之开关孔，上紧头尾之螺丝，将锁身固定，安装锁身前后锁盖去处。

The condensing tower comprises a gravity settling chamber where a set of loading trays is fixed, wherein the middle-lower part of the condensing tower mounts a round condenser tube, of which the upper and lower ends is connected with a sieve pore type baffle, in manner of closest arrangement based on the round section. The upper part of the condensing tower comprises a spherical cover without hem, a pre refrigerating chamber, a gas pipe type refrigerated trap, and the lower there of is a passive cold trap.

冷凝塔包括重力沉降室，沉降室内安有一组承料盘，冷凝塔中下部有按圆截面最紧密排列的方式设置圆形冷凝管，冷凝管上、下端联接有筛孔状隔板；冷冻塔上部分包括无折边球形塔盖，预冷冻室和气管式冷冻捕集器，下部分为无源冷阱捕集器。

Plunger valve is from the valve body, valve cover, stem, plunger, hole frame, sealing rings, hand wheels, of parts.

柱塞阀是由阀体、阀盖、阀杆、柱塞、孔架、密封环、手轮等零件组成。

4 Of the nominal size DN ≥ 200 of the valve, the valve cover can be processed in a screw, after trial, must be plugged with metal plug screw.

4.3.4对公称尺寸DN≥200的闸阀，可在阀盖上加工出一个螺孔，试验完毕后，必须用金属螺塞堵塞。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。