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The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The result showed that Ti02 nanowires with a diameter of 20 nm and a length up to several micron only can be synthesized in NH4F/glycol solution with a small amount of water (glycol: H20=39:1), 60V anodic voltage and 2h anodic time. The nanowires originated from the vertical splitting of anodically grown nanotubes.

结果表明，当醇水体积比为39：1时，60V电压下氧化2h，TiO2纳米管顶部的氧化层在缺陷处发生二次击穿溶解，产生自组装的孔核，场助溶解驱动力使纳米管顶部在孔核处沿管轴向垂直劈裂，形成直径20nm、长度可达几微米的TiO2纳米线。

A free flow abrasive hole polishing system and method improves air flow and resultant component cooling efficiency for an apertured article.

自由流动研磨孔抛光系统和改善多孔物品（18）空气流量和带孔物品有效组件冷却效率的方法。

The results of The biological characteristics of Semanotus bifasciatus have showed: 1. The hole of Semanotus bifasciatus associated with a certain temperature.2.The main damages of Semanotus bifasciatus located oriental arborvitae tree trunks, while above 3m parts are not damaged. 3.The Mating numbers of Semanotus bifasciatus are not unique ,and the duration varied due to the different individuals, the average duration is 3.2 minutes . 4. when are flying , they are more sensitive to blue and green; while moved, they are more sensitive to black.

对双条杉天牛的生物学特性的研究结果表明：双条杉天牛出孔与温度有一定的相关性，温度过低，双条杉天牛停止出孔；对于树高7m以上的树，主要危害侧柏基部的1-3m的主干部分，3m以上基本不受害；双条杉天牛需要经过一段时间的抱合才能交尾，交配对象不专一，雌雄成虫均可以多次交尾，交尾时间长短不一，平均3.2min/次；飞行时对蓝色和绿色较为敏感，爬行时对黑色较为敏感。

By the way of porous board to cut down on noise energy along with increasing of porosity, it could increase the attrited loss with sound power and reduce noise volume effectively.

使用开孔板的方式来削减声音能量，随著开孔率增多可增加与声音能量摩擦耗损之作用，可有效降低噪音音量。

The heat preservation effect of the awning can be seen after one awning. After a cycle of cold and warm seasons, an awninged embankment temperature is 3~5℃ lower than an ordinary embankment, and the permafrost table ascends 1.0~1.7m, with an average of 1.1m, under the berm, the road shoulder and the road center.

遮阳板措施的保温效果在应用只有1a的时间里面就表现出来，在第1个冷暖季节过后，在遮阳板保护下的路基土体温度就较普通路基低，在路基左路肩部位低出3~5℃；在路基的左右护道、路肩孔和中心孔冻土上限抬升在1.0~1.7m，平均抬升1.1m。

The biofouling in this area reflected distinct characteristics of subtropical harbors. A total of 82 fouling organism species were recorded, among which Balanus reticulates, Dexiosprira foraminosus, Spirorbis papillatus, Bugula neritina, Membranipora grandicella, Styela canopus, Electra tenella, Perna viridis and Dendostrea crenulifera were dominant species.

调查记录污损生物共82种，其中优势种为网纹藤壶Balanus reticulatus、有孔右旋虫Dexiosprira foraminosus、左旋虫Spirorbis papillatus、多室草苔虫Bugula neritina、大室膜孔苔虫Membranipora grandicella、美丽琥珀苔虫Electra tenella、缘齿牡蛎Dendostrea crenulifera、冠瘤海鞘Styela canopus和翡翠贻贝Perna viridis。

Also design method of blasthole spacing in pre-splitting blasting is discussed, and the writers hold that primary fractures should be considered in process of hole-spacing design.

同时还讨论了预裂炮孔间距的设计方法，认为在炮孔间距设计时，应考虑原生裂缝的存在。

Blasthole deflection is an severe adverse factor when mining is carried out using large diameter long hole blasting .

在大直径深孔采矿中，炮孔偏斜是非常不利的因素。

Blasthole deflection is an severe adverse factor when mining is carried out using large diame- ter long hole blasting.

在大直径深孔采矿中，炮孔偏斜是非常不利的因素。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。